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1.21.1.1: iodotyrosine deiodinase

This is an abbreviated version!
For detailed information about iodotyrosine deiodinase, go to the full flat file.

Word Map on EC 1.21.1.1

Reaction

3-iodo-L-tyrosine
+
NADP+
+
Iodide
=
3,5-diiodo-L-tyrosine
+
NADPH
+
H+

Synonyms

DEHAL1, Dehal1 protein, DEHAL1B, EC 1.22.1.1, iodotyrosine dehalogenase 1, iodotyrosine deiodinase, IYD, lyd, NADH oxidase/flavin reductase, SUP-18, SUP-18 IYD, TDH

ECTree

     1 Oxidoreductases
         1.21 Catalysing the reaction X-H + Y-H = X-Y
             1.21.1 With NAD+ or NADP+ as acceptor
                1.21.1.1 iodotyrosine deiodinase

Purification

Purification on EC 1.21.1.1 - iodotyrosine deiodinase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
HiTrap column chromatography
Ni-NTA column chromatography and 200 gel filtration
Ni2+ affinity column chromatography
purification of stable aporptoein
-
recombinant SUMO-tagged enzyme from Escherichia coli strain Rosetta 2(DE3) by nickel affinity chromatography, tag removal, and gel filtration
solubilization by 0.1% CHAPS