1.2.7.4: anaerobic carbon-monoxide dehydrogenase

This is an abbreviated version!
For detailed information about anaerobic carbon-monoxide dehydrogenase, go to the full flat file.

Reaction

Synonyms

acetyl coenzyme A synthase/carbon monoxide dehydrogenase, acetyl-CoA synthase/carbon monoxide dehydrogenase, ACS/CODH, Carbon dioxide/carbon monoxide oxidoreductase, Carbon monoxide dehydrogenase, carbon monoxide dehydrogenase-corrinoid enzyme complex, carbon monoxide dehydrogenase/acetyl-CoA synthase, Carbon monoxide oxidoreductase, Cdh, CO dehydrogenase, CO dehydrogenase complex, CO dehydrogenase complex II, CO dehydrogenase II, CO dehydrogenase/acetyl-CoA synthase, Co-DG, CO-DH, CODH, CODH II, CODH-II, CODH/ACS, CODHACS, CODHase, CODHII, coos, Dehydrogenase, carbon monoxide, EC 1.2.99.2, monoxide dehydrogenase/acetyl-CoA synthase, Ni,Fe-CODH, Ni,Fe-dependent carbon monoxide dehydrogenase, Ni-CODH, Ni-containing carbon monoxide dehydrogenase, Ni-Fe carbon monoxide dehydrogenase II

ECTree

     1 Oxidoreductases

         1.2 Acting on the aldehyde or oxo group of donors

             1.2.7 With an iron-sulfur protein as acceptor

                1.2.7.4 anaerobic carbon-monoxide dehydrogenase

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Results	in table
11	Activating Compound
8614	AA Sequence
1	Application
1	CAS Registry Number
12	Cloned(Commentary)
18	Cofactor
8	Crystallization (Commentary)
2	Expression
10	General Information
5	General Stability
63	Inhibitors
3	kcat/KM [mM/s]
18	Ki Value [mM]
32	KM Value [mM]
3	Localization
103	Metals/Ions
82	Molecular Weight [Da]
38	Natural Substrates/ Products (Substrates)
170	Organism
11	Oxidation Stability
11	Pathway
75	PDB ID
19	pH Optimum
5	pH Range
1	pH Stability
38	Protein Variants
28	Purification (Commentary)
13	Reaction
3	Reaction Type
135	Reference
12	Source Tissue
46	Specific Activity [micromol/min/mg]
6	Storage Stability
182	Substrates and Products (Substrate)
36	Subunits
138	Synonyms
1	Systematic Name
8	Temperature Optimum [°C]
1	Temperature Range [°C]
10	Temperature Stability [°C]
18	Turnover Number [1/s]

Temperature Stability

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Temperature Stability on EC 1.2.7.4 - anaerobic carbon-monoxide dehydrogenase

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TEMPERATURE STABILITY

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

104

Methanosarcina barkeri

-

10 min, complete loss of activity in presence or in absence of CO

390449

24

Methanosarcina barkeri

-

10 min, about 30% loss of activity in presence of CO, stable in absence of CO

390449

25 - 70

Moorella thermoacetica

-

purification can be done at 25°C without loss of activity, heating for 30 min at 67°C leads to apparent loss, heating for only a few min at 70°C activates the enzyme

390452

39

Methanosarcina barkeri

-

10 min, 45% loss of activity in presence of CO, stable in absence of CO

390449

60

Methanothrix soehngenii

-

10 min, about 10% loss of activity

390473

62

Methanosarcina barkeri

-

10 min, about 65% loss of activity in presence of CO, about 5% loss of activity in absence of CO

390449

72

Methanothrix soehngenii

-

10 min, complete inactivation

390473

80

Methanosarcina barkeri

-

10 min, about 85% loss of activity in presence of CO, about 20% loss of activity in absence of CO

390449

80 - 104

Methanosarcina barkeri

-

most of the activity remains after heating at 80°C, nearly complete inactivation after heating at 104°C, greater loss of activity at all temperatures when heated in presence of CO

390449

additional information

Methanosarcina barkeri

-

CO renders the enzyme more susceptible to temperature inactivation

390449