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1.2.7.1: pyruvate synthase

This is an abbreviated version!
For detailed information about pyruvate synthase, go to the full flat file.

Word Map on EC 1.2.7.1

Reaction

pyruvate
+
CoA
 +
oxidized ferredoxin
=
acetyl-CoA
 +
CO2
 +
reduced ferredoxin
 + 2 H+

Synonyms

2-ketobutyrate synthase, 2-oxobutyrate (methylviologen), 2-oxobutyrate synthase (benzylviologen), 2-oxobutyrate-ferredoxin oxidoreductase, alpha-ketobutyrate synthase, alpha-ketobutyrate-ferredoxin oxidoreductase, AP120, Ape2126/2128, bifunctional pyruvate decarboxylase/pyruvate ferredoxin oxidoreductase, EC 1.2.7.2, Moth_0064, NifJ1, OFOR, PFO, PFO A, PFOR, Pfor1, PforA, PFR, PFR1, POR, PorEDABG, pyruvate ferredoxin oxidoreductase, pyruvate-ferredoxin oxidoreductase, pyruvate:Fd oxidoreductase, pyruvate:ferredoxin oxidoreductase, pyruvate:ferredoxin oxidoreductase A, synthase, 2-oxobutyrate, TKV_c04340, Tsac_0046, TTE0445

ECTree

     1 Oxidoreductases
         1.2 Acting on the aldehyde or oxo group of donors
             1.2.7 With an iron-sulfur protein as acceptor
                1.2.7.1 pyruvate synthase

Purification

Purification on EC 1.2.7.1 - pyruvate synthase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
DE52 column chromatography, DEAE-cellulose column chromatography, and Sephadex G25 gel filtration
-
enzyme Ape2126/2128
Ni-NTA column chromatography
nickel affinity column chromatography
-
purifiaction using Q-sepharose, hydroxyapatite, blue sepharose and sephacryl
-
purification from cells grown on glucose
purification under strictly unaerobic conditions
-
purified enzyme shos a brownish colour characteristic of iron-sulfur containing proteins
Q56317; 05650; Q56316; O05651
purified enzyme shows a brownish colour characteristic of iron-sulfur containing proteins
Q-Sepharose column chromatography, phenyl Sepharose column chromatography, and Superdex 200 gel filtration
-
recombinantly expressed delta subunit is purified after reconstitution with iron and sulfide. The reconstituted delta-subunit is monomeric with a mass of 11879 Da as determined by mass spectrometry. The purified and reconstituted delta subunit contains 8 Fe mol/mol and remains intact when incubated at 85 °C for 2 h
-