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1.2.3.4: oxalate oxidase

This is an abbreviated version!
For detailed information about oxalate oxidase, go to the full flat file.

Word Map on EC 1.2.3.4

Reaction

oxalate
+
O2
= 2 CO2 +
H2O2

Synonyms

aero-oxalo dehydrogenase, Germin, Germin GF-2.8, Germin GF-3.8, germin-like oxidase, germin-like protein, gl-OXO, glp1, GLP2, HvOxo1, oxalate oxidase, oxalate oxidase GF-2.8, oxalate: O2 oxidoreductase, oxalate:oxygen oxidoreductase, oxalic acid oxidase, OXO, OXO-G, OXO1, OXO2, OXO3, OXO4, OxOx, TaOxo1, TaOxo2

ECTree

     1 Oxidoreductases
         1.2 Acting on the aldehyde or oxo group of donors
             1.2.3 With oxygen as acceptor
                1.2.3.4 oxalate oxidase

General Stability

General Stability on EC 1.2.3.4 - oxalate oxidase

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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
after 90 min treatment with trypsin 10% activity retained by free protein, 35% activity retained by immobilized protein on PEG1900 and 30% activity retained by immobilized protein on PEG5000
-
based on the absoption changes at 325 nm, it is possible to estimate the half-life of the Mn5+ species at room temperature: t1/2 = 42 h (pH 4) or 95 h (pH 7)
-
enzyme activity of the C isoform is lost in the presence of sodium dodecyl sulfate (SDS)
-
immobilized on alkylamine glass
-
immobilized on PEG1900 and PEG5000
-
immobilized on zirkonia coated alkylamine glass, retains 97% of enzyme activity
-
isoforms OxO1-4 are resistant to a treatment with pepsin at 37°C for 30 min at pH 2.0, while the activity of isoform OxO4 is actually increased on pepsin treatment
-
the immobilized enzyme on modified mica chip retains 65.35% of the specific activity of free enzyme
-
the polyvinyl chloride-immobilized enzyme retains 65% of specific activity of free enzyme
-