1.2.1.75: malonyl-CoA reductase (malonate semialdehyde-forming)
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For detailed information about malonyl-CoA reductase (malonate semialdehyde-forming), go to the full flat file.
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Synonyms
bi-functional malonyl-CoA reductase, malonate semialdehyde reductase, malonate-semialdehyde dehydrogenase, malonyl CoA reductase (malonate semialdehyde-forming), malonyl-CoA reductase, MCR, More, MSAR
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Cloned
Cloned on EC 1.2.1.75 - malonyl-CoA reductase (malonate semialdehyde-forming)
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Escherichia coli Rosetta 2 cells transformed with pTrc99A-Mcr plasmid harbouring mcr gene from Sulfolobus tokodaii
expressed in Escherichia coli BL21(DE3) cells
gene mcr, recombinant expression in Escherichia coli strain BL21(DE3), coexpression of acetyl-CoA synthetase
gene mcr, recombinant expression of MCR enzyme mutant in Escherichia coli strain CE43(DE3), coexpression with malonyl-CoA synthetase (MatB) from Rhodopseudomonas palustris, native transhydrogenase (PntAB), and NAD kinase (YfjB)
gene mcr, recombinant expression of the Chloroflexus aurantiacus enzyme in Methylosinus trichosporium strain OB3b, subcloning in Escherichia coli strain DH5alpha
gene mcr, separate recombinant expression of the N- and C-terminal subdomains of the enzyme, MCR-C and MCR-N, in Schizosaccharomyces pombe strain FY12804/NBRP, coexpression of acetyl-CoA synthase, gene acsSE, and aldehyde dehydrogenase, gene atd1, under the control of the cam1 promoter