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1.2.1.67: vanillin dehydrogenase

This is an abbreviated version!
For detailed information about vanillin dehydrogenase, go to the full flat file.

Word Map on EC 1.2.1.67

Reaction

Vanillin
+
NAD+
+
H2O
=
vanillate
+
NADH
+
H+

Synonyms

ALDH, aromatic aldehyde dehydrogenase, CD36-03230, Cd36-03230p, DesV, DesV-like ALDH, LigV, ro02986, SLG_07060, SLG_28320, SN dehydrogenase, syringaldehyde dehydrogenase, vanillin dehydrogenase, VDH, VDH1, VN dehydrogenase, YfmT

ECTree

     1 Oxidoreductases
         1.2 Acting on the aldehyde or oxo group of donors
             1.2.1 With NAD+ or NADP+ as acceptor
                1.2.1.67 vanillin dehydrogenase

Cloned

Cloned on EC 1.2.1.67 - vanillin dehydrogenase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloned into the pET-28a expression vector and transformed into Escherichia coli
cosmid library of SYK-6 strain transferred to Sphingomonas paucimobilis IAM12578. LigV expressed in Escherichia coli BL21(DE3)
expressed in Escherichia coli BL21(DE3) cells
expression in Escherichia coli
gene CD36-03230, recombinant expression in Escherichia coli
gene desV or SLG_28320, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3), recombinant expression of desV disruption mutants
gene ligV or SLG_07060, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli BL21(DE3)
gene vdh, phylogenetic tree
gene yfmT, DNA and amino acid sequence determination and analysis, recombinant overexpression of C-terminally HIs6-tagged enzyme in Escherchia coli strain JM109/pNG533.1
-
vdh gene cloned into pET23a(+)vector heterologously expressed with C-terminal His6-tag in Escherichia coli BL21(DE3) cells