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1.2.1.61: 4-hydroxymuconic-semialdehyde dehydrogenase

This is an abbreviated version!
For detailed information about 4-hydroxymuconic-semialdehyde dehydrogenase, go to the full flat file.

Reaction

4-hydroxymuconic semialdehyde
+
NAD+
+
H2O
=
maleylacetate
+
NADH
+
H+

Synonyms

4-HS dehydrogenase, 4-hydroxymuconic semialdehyde dehydrogenase, gamma-hydroxymuconic semialdehyde dehydrogenase, hAPE, HqdC, NAD dependent gamma-hydroxymuconic semialdehyde dehydrogenase, PdcG, pnpE

ECTree

     1 Oxidoreductases
         1.2 Acting on the aldehyde or oxo group of donors
             1.2.1 With NAD+ or NADP+ as acceptor
                1.2.1.61 4-hydroxymuconic-semialdehyde dehydrogenase

Purification

Purification on EC 1.2.1.61 - 4-hydroxymuconic-semialdehyde dehydrogenase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
His6-PdcG by Ni2+-NTA affinity chromatography
-
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity and anion exchange chromatography, followed by gel filtration