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1.2.1.44: cinnamoyl-CoA reductase

This is an abbreviated version!
For detailed information about cinnamoyl-CoA reductase, go to the full flat file.

Word Map on EC 1.2.1.44

Reaction

cinnamaldehyde
+
CoA
 +
NADP+
=
cinnamoyl-CoA
 +
NADPH
 +
H+

Synonyms

AtCCR1, BpCCR1, CCR, CCR1, CCR12, CCR14 isoform, CCR17, CCR19, CCR2, CCR2-1, CCR20, CCR21, CCR3, CCR7, CCRH1, cinnamoyl CoA reductase, cinnamoyl CoA reductase 1, cinnamoyl coenzyme A reductase, cinnamoyl-Co-enzyme A reductase, cinnamoyl-CoA reductase, cinnamoyl-CoA reductase 1, cinnamoyl-CoA reductase1, cinnamoyl-CoA reductase2, cinnamoyl-CoA:NADPH reductase, cinnamoyl-coenzyme A reductase, cinnamoyl-coenzyme A reductase 1, FaCCR, feruloyl coenzyme A reductase, feruloyl-CoA reductase, ferulyl-CoA reductase, HcCCR1, HcCCR2, Ll-CCRH1, OsCCR1, p-hydroxycinnamoyl coenzyme A reductase, Ph-CCR1, PtCCR, PtoCCR1, PtoCCR7, PvCCR1, PvCCR2, reductase, cinnamoyl coenzyme A, SbCCR1, SbCCR2-1, SbCCR2-2, Ta-CCR2, VcCCR

ECTree

     1 Oxidoreductases
         1.2 Acting on the aldehyde or oxo group of donors
             1.2.1 With NAD+ or NADP+ as acceptor
                1.2.1.44 cinnamoyl-CoA reductase

Purification

Purification on EC 1.2.1.44 - cinnamoyl-CoA reductase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
as His-tagged enzyme by means of Ni-NTA beads
by gel filtration, to homogeneity
CCR protein is purified using a Bug-Buster GSTclosed circleBind purification kit, and the fused tag is cleaved and removed using the Enterokinase Cleavage capture kit
Ni-NTA agarose column chromatography
partial, two forms
-
recombinant fusion proteins GST-AtCCR1 and GST-AtCCR2, expressed in Escherichia coli
-
recombinant His-tagged enzyme CCR from Escherichia coli by nickel affinity chromatography and gel filtration
recombinant His-tagged enzyme CCR1 from Escherichia coli strain M15 by nickel affinity chromatography
recombinant His-tagged enzyme CCR1 from Escherichia coli strain Rosetta (DE3) by nickel affinity chromatography, anion exchange chromatography, and dialysis
recombinant His-tagged wild-type and mutant enzymes CCR7 from Escherichia coli strain M15 by nickel affinity chromatography
recombinant His6-tagged enzyme from Escherichia coli by nickel affinity chromatography and gel filtration
recombinant proteins are purified on a glutathione resin
using a glutathione-sepharose 4 Fast Flow column, purified GST-AtCCR1 is cleaved by thrombin to remove the GST tag
-
using Ni-NTA His-Bind resin