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1.2.1.36: retinal dehydrogenase

This is an abbreviated version!
For detailed information about retinal dehydrogenase, go to the full flat file.

Word Map on EC 1.2.1.36

Reaction

retinal
+
NAD+
+
H2O
=
retinoate
+
NADH
+
H+

Synonyms

aldehyde dehydrogenase 1, aldehyde dehydrogenase 1A1, aldehyde dehydrogenase 1A2, aldehyde dehydrogenase 1A3, ALDH 1A1, ALDH 1A2, ALDH class 1, ALDH-1A1, ALDH-3A1, ALDH1, ALDH1A1, Aldh1a2, Aldh1a3, BcALDH, cytosolic retinal dehydrogenase, dehydrogenase, retinal, dehydrogenase/reductase member 3, DHRS3, ETA-crystallin, More, NAD+-dependent aldehyde dehydrogenase, NAD-dependent retinal dehydrogenase, NAD-dependent retinal dehydrogenases, PB-RALDH, phenobarbital-induced aldehyde dehydrogenase, RALDH, RALDH-1, RALDH-3, RalDH1, RALDH1A1, RALDH2, RALDH3, Raldh4, RDH12, RDH13, RDH5, retialdehyde dehydrogenase, Retinal dehydrogenase, retinal dehydrogenase 1, retinal dehydrogenase 2, retinal dehydrogenase 3, retinal dehydrogenase 4, retinal dehydrogenase 5, retinal dehydrogenase type-2, retinal dehydrogenase-2, retinal dehydrogenase/reductase, retinal short-chain dehydrogenase/reductase, retinalaldehyde dehydrogenase 2, retinaldehyde dehydrogenase, retinaldehyde dehydrogenase 1, retinaldehyde dehydrogenase 2, retinaldehyde dehydrogenase 3, retinaldehyde dehydrogenase type II, retinaldehyde dehydrogenase type-1, retinaldehyde dehydrogenase-2, retinaldehyde dehydrogenases, retinol dehydrogenase 13, retSDR1, testis-specific aldehyde dehydrogenase, thyroid-hormone-binding protein, type 2 retinaldehyde dehydrogenase, xCTBP, xCTBP/xALDH1, zRalDH

ECTree

     1 Oxidoreductases
         1.2 Acting on the aldehyde or oxo group of donors
             1.2.1 With NAD+ or NADP+ as acceptor
                1.2.1.36 retinal dehydrogenase

Purification

Purification on EC 1.2.1.36 - retinal dehydrogenase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
after recombinant production in Escherichia coli BL-21DE3
by gel filtration, more than 400fold with a yield of about 1%, to near homogeneity
-
Ni-affinity chromatography
-
Ni-NTA column chromatography
Ni2+-NTA chelating Sepharose column chromatography
nickel chelating resin column chromatography and Superdex 200 gel filtration
nickel-Sepharose column chromatography and Q-Sepharose column chromatography
on a Ni-NTA column
partially by isoelectric focusing
-
purification of the enzyme(s) responsible for the GSH/DHLA-dependent NAD+ reduction activity from large-scale cell cultures, native enzyme from EKVX lung cancer cells by ultracentrifugation of crude cell extract and anion echange chromatography, hydrophobic interaction and hydroxyapatite chromatography, followed by gel filtration and another different step of anion exchange chromatography
RDH13-His6 purified by Ni2+ affinity chromatography, to homogeneity
-
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3)pLys by nickel affinity chromatography
recombinant isozyme type-1 as fusion protein from Escherichia coli by GST-affinity chromatography, cleavage by thrombin to eliminate the fusion tag
-
recombinant isozyme type-2 as fusion protein from Escherichia coli by GST-affinity chromatography, cleavage by thrombin to eliminate the fusion tag
-
recombinant N-terminally His-tagged enzyme from Escherichia coli strain C41(DE3) by nickel affinity chromatography
recombinant N-terminally His-tagged wild-type and mutant enzymes from Escherichia coli strain C41(DE3) by nickel affinityy chromatography
recombinant tagged enzyme from Escherichia coli strain ER2566 by metal chelating affinity chromatography, desalting gel filtration, and dialysis
recombinant wild-type and mutant isozyme type-2 from Escherichia coli strain Bl21(DE3), no usage of His-tag since it may influence the enzyme activity
-
using a nickel-chelating Sepharose and a S300 HR gel-filtration column