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0.023
(3,4-dihydroxyphenyl)(hydroxy)acetaldehyde
-
membrane-bound isoenzyme ALDH
0.001
(R)-trans-4-hydroxy-2-nonenal
ALDH2, in 40 mM sodium phosphate buffer (pH 7.4), at 37°C
0.0007
(S)-trans-4-hydroxy-2-nonenal
ALDH2, in 40 mM sodium phosphate buffer (pH 7.4), at 37°C
0.0358
1,N6-ethenoadenine dinucleotide
-
isoenzyme E3
0.00015 - 0.0016
1-formyl-6-methylpyrene
0.000027 - 0.004
1-formyl-8-methylpyrene
0.0009 - 0.004
1-formylpyrene
0.0000032
2,4-dinitrobenzaldehyde
-
enzyme ALDH-2
0.00038 - 0.011
2-formylpyrene
0.000008 - 0.00046
2-naphthaldehyde
0.00024
2-naphthalene carboxylate
-
apparent Km value, diphosphate buffer, pH 8.1, cofactor NADH
0.0053
2-nonenal
-
pH and temperature not specified in the publication
0.0005 - 0.017
3,4-dihydroxyphenyl acetaldehyde
0.0004 - 0.0042
3,4-dihydroxyphenylacetaldehyde
0.00033
3,4-dimethoxybenzaldehyde
-
enzyme ALDH-2
0.022
3-acetylpyridine-NAD+
-
-
0.29 - 0.49
3-hydroxypropionaldehyde
0.12
3-hydroxypropionic acid
-
with NADH as cofactor, in 50 mM potassium phosphate buffer (pH 8.0), at 37°C
3.3
3-oxopropyl phosphonate
wild type enzyme, at pH 7.5 and 30°C
0.00196
3-Pyridinecarboxaldehyde
-
enzyme ALDH-2
0.0001 - 0.0003
4-(dimethylamino)cinnamaldehyde
0.005 - 0.014
4-aminobutyrate
0.014
4-cyanobenzaldehyde
-
-
0.0042
4-Dimethylaminobenzaldehyde
-
recombinant ALDH3A1
0.00004 - 0.00078
4-formylpyrene
3.383
4-hydroxy-2-nonenal
-
at 25°C, pH not specified in the publication
0.155
4-hydroxy-3-methoxybenzaldehyde
-
recombinant ALDH3A1
0.0021 - 0.0403
4-hydroxynonenal
0.000065
4-methoxy-1-naphthaldehyde
-
enzyme ALDH-2
0.019
4-methoxybenzaldehyde
-
recombinant ALDH3A1
0.0002
4-methyoxy-1-naphthaldehyde
-
enzyme ALDH-1
0.0043
4-nitrophenyl acetate
-
-
0.0000063
5-(dimethylamino)-2-naphthaldehyde
-
enzyme ALDH-1
0.0000004 - 0.0000025
5-bromo-1-naphthaldehyde
0.026
5-hydroxyindol acetaldehyde
-
membrane-bound isoenzyme ALDH
0.0189
5-methoxyindole-3-carboxaldehyde
-
enzyme ALDH-2
0.0000004 - 0.000011
5-nitro-1-naphthaldehyde
0.00069
6,7-dimethoxycoumarin-3-carboxaldehyde
-
enzyme ALDH-2
0.0000023
6-(dimethylamino)-2-naphthaldehyde
-
enzyme ALDH-2
0.0017 - 0.02
6-dimethylamino-2-naphthaldehyde
0.00016 - 0.0002
6-methoxy-2-naphthaldehyde
0.0054
6-methoxy-2-quinolinone-4-carboxaldehyde
-
enzyme ALDH-2
0.00213
7-(dimethylamino)-2-quinolinone-4-carboxaldehyde
-
enzyme ALDH-2
0.000062
7-(dimethylamino)-coumarin-3-carboxaldehyde
-
enzyme ALDH-2
0.00006
7-acetoxycoumarin-3-carboxaldehyde
-
enzyme ALDH-2
0.15
7-hydroxycoumarin-3-carboxaldehyde
-
enzyme ALDH-2
0.05
7-methoxy-2-quinolinone-4-carboxaldehyde
-
enzyme ALDH-2
0.00028
7-methoxycoumarin-3-carboxaldehyde
-
enzyme ALDH-2
0.00005 - 80
acetaldehyde
0.017
acetylaldehyde
-
at substrate concentrations 0-0.07 mM
0.009
acrolein
-
wild type isoform ALDH1A1, in 100 mM sodium diphosphate (pH 9.5), at 25°C
0.0093
all-trans-retinal
-
at pH 8.0 and 25°C
0.064 - 0.63
alpha-aminoadipate semialdehyde
0.00093
alpha-phenylpropanal
-
enzyme ALDH-2
0.000018 - 50
benzaldehyde
0.0411 - 0.26
Betaine aldehyde
0.0234
betaNAD+
-
isoenzyme E3
0.26 - 1.74
Butyraldehyde
0.00055
cinnamaldehyde
-
-
0.006
cinnamic aldehyde
-
recombinant ALDH3A1
1.136
D-glyceraldehyde
-
-
8.51
DL-glyceraldehyde
-
in 50 mM Tris/HCl, at pH 8.0 and 80°C
6.12
DL-glyceraldehyde 3-phosphate
-
in 50 mM Tris/HCl, at pH 8.0 and 80°C
0.000054
fluorene-2-carboxaldehyde
-
enzyme ALDH-1
0.0009 - 0.56
Glutaraldehyde
0.183
glutathione-4-oxonon-2-enal
-
-
0.17 - 7.374
glyceraldehyde
0.097 - 0.372
glyceraldehyde 3-phosphate
0.046
glycoaldehyde
-
isoenzyme 2, pH 7.4
0.005 - 0.69
glycolaldehyde
0.000018 - 0.035
Heptanal
2.9
hexanaldehyde
at pH 8.0 and 21°C
0.0005
hydrocinnamaldehyde
-
enzyme ALDH-2
0.0007 - 0.001
indole 3-acetaldehyde
0.00015 - 0.002
Indole-3-acetaldehyde
0.01 - 0.02
Indole-3-aldehyde
-
enzyme ALDH-2
0.21 - 0.68
Isovaleraldehyde
0.00009
m-methoxybenzaldehyde
-
enzyme ALDH-2
0.000018
m-methylbenzaldehyde
-
enzyme ALDH-2
0.0043 - 0.466
malondialdehyde
0.0086 - 1.876
methylglyoxal
0.043
Monochloroacetaldehyde
-
-
0.11
N-acetyl-4-aminobutyrate
-
enzyme from liver
0.944
N-guanine dinucleotide
-
isoenzyme E3
0.0008 - 0.0285
n-nonanal
0.088
NADH
-
at pH 7.0 and 55°C
0.0008
o-methoxybenzaldehyde
-
enzyme ALDH-2
1.3
o-methylbenzaldehyde
-
enzyme ALDH-2
0.0000063
o-nitrobenzaldehyde
-
enzyme ALDH-2
0.172
oxidized nicotinamide hypoxanthine dinucleotide
-
isoenzyme E3
0.00002
p-(dimethylamino)-benzaldehyde
-
enzyme ALDH-2
0.00006
p-(dimethylamino)benzaldehyde
-
enzyme ALDH-1
0.000005 - 0.00142
p-(dimethylamino)cinnamaldehyde
0.024
p-Carboxybenzaldehyde
-
-
0.000018 - 0.0004
p-Methoxybenzaldehyde
0.000017
p-methylbenzaldehyde
-
enzyme ALDH-2
0.000007 - 2
p-nitrobenzaldehyde
0.0000007
p-nitrocinnamaldehyde
-
enzyme ALDH-2
0.000034 - 0.006
pentanal
2.2
pentanaldehyde
at pH 8.0 and 21°C
0.000004
phenanthrene-9-carboxaldehyde
-
enzyme ALDH-2
0.000029 - 0.027
phenylacetaldehyde
0.0032 - 0.5
phosphonoacetaldehyde
0.0028 - 12
propionaldehyde
0.013
Pyruvic aldehyde
-
-
0.00033
quinoline-3-carboxaldehyde
-
enzyme ALDH-2
0.0028
quinoline-4-carboxaldehyde
-
enzyme ALDH-2
0.69 - 3.54
Succinic semialdehyde
0.18 - 0.199
trans-2-hexenal
0.003 - 0.0322
trans-2-nonenal
0.000035 - 0.0004
trans-cinnamaldehyde
0.14 - 0.24
Valeraldehyde
additional information
additional information
-
0.00015
1-formyl-6-methylpyrene
ALDH2, at pH 7.5 and 37°C
0.0016
1-formyl-6-methylpyrene
in 0.1 M sodium diphosphate buffer (pH 8.5), at ambient temperature
0.000027
1-formyl-8-methylpyrene
ALDH2, at pH 7.5 and 37°C
0.004
1-formyl-8-methylpyrene
in 0.1 M sodium diphosphate buffer (pH 8.5), at ambient temperature
0.0009
1-formylpyrene
ALDH2, at pH 7.5 and 37°C
0.004
1-formylpyrene
in 0.1 M sodium diphosphate buffer (pH 8.5), at ambient temperature
0.00038
2-formylpyrene
ALDH2, at pH 7.5 and 37°C
0.011
2-formylpyrene
in 0.1 M sodium diphosphate buffer (pH 8.5), at ambient temperature
0.000008
2-naphthaldehyde
-
enzyme ALDH-2
0.0004
2-naphthaldehyde
-
recombinant ALDH3A1
0.00046
2-naphthaldehyde
-
salivary ALDH
0.0005
3,4-dihydroxyphenyl acetaldehyde
-
isoenzyme II
0.001
3,4-dihydroxyphenyl acetaldehyde
-
pH 7.1, isoenzyme 2
0.003
3,4-dihydroxyphenyl acetaldehyde
-
isoenzyme III
0.017
3,4-dihydroxyphenyl acetaldehyde
-
membrane-bound isoenzyme ALDH
0.0004
3,4-dihydroxyphenylacetaldehyde
isozyme ALDH1A1 from liver
0.001
3,4-dihydroxyphenylacetaldehyde
isozyme ALDH2 from liver
0.0042
3,4-dihydroxyphenylacetaldehyde
isozyme ALDH1A1 from brain
0.29
3-hydroxypropionaldehyde
-
with NADP+ as cofactor, in 50 mM potassium phosphate buffer (pH 8.0), at 37°C
0.48
3-hydroxypropionaldehyde
-
recombinant enzyme, in 50 mM potassium phosphate buffer, at pH 8.0 and 45°C
0.49
3-hydroxypropionaldehyde
-
with NAD+ as cofactor, in 50 mM potassium phosphate buffer (pH 8.0), at 37°C
0.0001
4-(dimethylamino)cinnamaldehyde
-
native enzyme
0.0003
4-(dimethylamino)cinnamaldehyde
-
mutant enzyme S74A
0.005
4-aminobutyrate
-
enzyme from liver
0.007
4-aminobutyrate
-
enzyme from brain
0.008
4-aminobutyrate
-
minor component of enzyme E3, pH 7.4
0.014
4-aminobutyrate
-
major component of enzyme E3, pH 7.4
0.00004
4-formylpyrene
ALDH2, at pH 7.5 and 37°C
0.00078
4-formylpyrene
in 0.1 M sodium diphosphate buffer (pH 8.5), at ambient temperature
0.0021
4-hydroxynonenal
-
pH 8.0, 25°C
0.0179
4-hydroxynonenal
-
pH 8.0, 25°C
0.0403
4-hydroxynonenal
-
in 100 mM sodium diphosphate, at pH 8.0 and 22°C
0.0000004
5-bromo-1-naphthaldehyde
-
enzyme ALDH-2
0.0000025
5-bromo-1-naphthaldehyde
-
enzyme ALDH-1
0.0000004
5-nitro-1-naphthaldehyde
-
enzyme ALDH-2
0.000011
5-nitro-1-naphthaldehyde
-
enzyme ALDH-1
0.0017
6-dimethylamino-2-naphthaldehyde
-
apparent Km value, pyrophosphate buffer, pH 8.1, cofactor NAD+
0.0072
6-dimethylamino-2-naphthaldehyde
-
salivary ALDH
0.0079
6-dimethylamino-2-naphthaldehyde
-
apparent Km value, phosphate buffer, pH 7.3, cofactor NAD+
0.0086
6-dimethylamino-2-naphthaldehyde
-
apparent Km value, phosphate buffer, pH 6.2, cofactor NAD+
0.0114
6-dimethylamino-2-naphthaldehyde
-
apparent Km value, phosphate buffer, pH 8.0, cofactor NAD+
0.02
6-dimethylamino-2-naphthaldehyde
-
recombinant ALDH3A1
0.00016
6-methoxy-2-naphthaldehyde
-
recombinant ALDH3A1
0.0002
6-methoxy-2-naphthaldehyde
-
salivary ALDH
0.00005
acetaldehyde
-
major component of enzyme E3
0.0001
acetaldehyde
-
isoenzyme 1
0.0002
acetaldehyde
-
isoenzyme F2
0.00031
acetaldehyde
-
pH 9.5
0.00036
acetaldehyde
-
pH 7.5
0.0004
acetaldehyde
-
native enzyme
0.0004
acetaldehyde
ALDH2, in 40 mM sodium phosphate buffer (pH 7.4), at 37°C
0.00048
acetaldehyde
ALDH2, at pH 7.5 and 37°C
0.00065
acetaldehyde
-
pH 8.0, 25°C, wild-type enzyme
0.002
acetaldehyde
-
pH 9.0, isoenzyme E2
0.002 - 0.003
acetaldehyde
-
isoenzyme 2
0.0024
acetaldehyde
-
isoenzyme 2, pH 9.5
0.0027
acetaldehyde
-
mutant enzyme S74A
0.003
acetaldehyde
-
isoenzyme 2, pH 7.0
0.003
acetaldehyde
-
isoenzyme E2
0.003
acetaldehyde
-
pH 7.4, isoenzyme E2
0.0032
acetaldehyde
0.1 M sodium diphosphate pH 8.0, 1 mM NAD+, 50 mM 2-mercaptoethanol, 1 mM pyrazole, 30 min, 25°C
0.0035
acetaldehyde
-
isoenzyme ALDH2 from normal liver
0.005
acetaldehyde
-
isoenzyme ALDH-II
0.0066
acetaldehyde
-
in 50 mM KH2PO4-K2HPO4 buffer (pH 8.0), at 60°C
0.00879
acetaldehyde
-
pH 8.0, 25°C, heteromeric enzyme obtained by coexpression of ALDH2 and ALDH2(2) in Escherichia coli
0.009
acetaldehyde
-
pH 9.5
0.01
acetaldehyde
-
100 mM Na2HPO4, pH 7.4, 100 mM NaCl, 1 mM NAD+, 25°C
0.0115
acetaldehyde
-
0.1 M sodium diphosphate pH 8.0, 1 mM NAD+, 50 mM 2-mercaptoethanol, 1 mM pyrazole, 30 min, 25°C
0.022
acetaldehyde
-
isoenzyme ALDH1 from normal liver
0.02573
acetaldehyde
-
pH 8.0, 25°C, mutant enzyme ALDH2(2)
0.03
acetaldehyde
-
isoenzyme E1
0.04
acetaldehyde
-
enzyme from brain
0.048
acetaldehyde
-
isoenzyme III
0.05
acetaldehyde
-
enzyme from liver
0.055
acetaldehyde
-
at 25°C, pH not specified in the publication
0.07
acetaldehyde
-
isoenzyme F1
0.12
acetaldehyde
-
isoenzyme 1, pH 9.5
0.18
acetaldehyde
-
enzyme form ALDH-1, pH 9.5
0.18
acetaldehyde
-
enzyme ALDH-1, pH 7.5
0.2
acetaldehyde
-
enzyme ALDH-2, pH 9.5
0.22
acetaldehyde
-
isoenzyme ALDH-I
0.83
acetaldehyde
-
at substrate concentrations 0.8-20 mM
1
acetaldehyde
-
with NAD+ as cofactor, in 50 mM potassium phosphate buffer (pH 8.0), at 37°C
1.6
acetaldehyde
-
at pH 7.0 and 55°C
2.4
acetaldehyde
-
enzyme I from microsome
2.4
acetaldehyde
-
at pH 8.0 and 25°C
2.5
acetaldehyde
-
isoenzyme 1, pH 7.0
5.16
acetaldehyde
at pH 8.0 and 21°C
5.8
acetaldehyde
ALDH5A, in 40 mM sodium phosphate buffer (pH 7.4), at 37°C
6.38
acetaldehyde
-
in 50 mM Tris/HCl, at pH 8.0 and 80°C
10
acetaldehyde
-
membrane-bound isoenzyme ALDH
80
acetaldehyde
in 0.1 M sodium diphosphate buffer (pH 8.5), at ambient temperature
0.064
alpha-aminoadipate semialdehyde
-
pH 8.0, 26°C, recombinant wild-type enzyme
0.169
alpha-aminoadipate semialdehyde
-
pH and temperature not specified in the publication
0.63
alpha-aminoadipate semialdehyde
-
pH 8.0, 26°C, recombinant mutant A505P
0.000018
benzaldehyde
-
enzyme ALDH-2
0.00006
benzaldehyde
-
pH 9.5
0.0001
benzaldehyde
-
native enzyme
0.0002
benzaldehyde
-
mutant enzyme S74A
0.0399
benzaldehyde
-
at 25°C and pH 8.5, with NADP+ as the electron acceptor
0.05
benzaldehyde
-
at 25°C, pH not specified in the publication
0.0634
benzaldehyde
-
at 25°C and pH 8.5, with NAD+ as the electron acceptor
0.148
benzaldehyde
-
recombinant ALDH3A1
0.15
benzaldehyde
-
100 mM Na2HPO4, pH 7.4, 100 mM NaCl, 1 mM NAD+, 25°C
0.16
benzaldehyde
-
salivary ALDH
0.23
benzaldehyde
in 0.1 M sodium diphosphate buffer (pH 8.5), at ambient temperature
0.5302
benzaldehyde
-
pH and temperature not specified in the publication
1.3
benzaldehyde
-
phenobarbital-inducible enzyme
1.6
benzaldehyde
-
enzyme I from mitochondria
3
benzaldehyde
-
enzyme II from microsomes
5.37
benzaldehyde
-
with NADP+ as cofactor, in 50 mM potassium phosphate buffer (pH 8.0), at 37°C
5.9
benzaldehyde
-
enzyme I from microsomes
50
benzaldehyde
-
enzyme II from mitochondria
0.0411
Betaine aldehyde
-
pH and temperature not specified in the publication
0.09
Betaine aldehyde
-
minor component of enzyme E3, pH 7.4
0.14
Betaine aldehyde
-
enzyme from brain
0.26
Betaine aldehyde
-
enzyme from liver
0.26
Betaine aldehyde
-
major component of enzyme form E3, pH 7.4
0.00019
Butanal
-
-
0.0007
Butanal
-
native enzyme
0.002
Butanal
-
mutant enzyme S74A
0.0038
Butanal
1 mM NAD+, 0,05 mM substrate, 250 mM sodium bicarbonate-NaOH buffer pH 10.0, 37°C
0.26
Butyraldehyde
-
recombinant enzyme, in 50 mM potassium phosphate buffer, at pH 8.0 and 45°C
0.97
Butyraldehyde
-
with NAD+ as cofactor, in 50 mM potassium phosphate buffer (pH 8.0), at 37°C
1.74
Butyraldehyde
at pH 8.0 and 21°C
0.0000029
decanal
-
enzyme form ALDH-1, pH 9.5
0.000022
decanal
-
enzyme form ALDH-2, pH 9.5
0.005
dodecanal
-
in 100 mM sodium diphosphate, at pH 8.0 and 22°C
0.02
dodecanal
-
microsomal enzyme
0.031
formaldehyde
-
-
0.178
formaldehyde
-
native enzyme
0.32
formaldehyde
-
enzyme ALDH-2
0.333
formaldehyde
1 mM NAD+, 0,05 mM substrate, 250 mM sodium bicarbonate-NaOH buffer pH 10.0, 37°C
0.42
formaldehyde
ALDH2, at pH 7.5 and 37°C
0.7
formaldehyde
-
mutant enzyme S74A
0.0009
Glutaraldehyde
-
native enzyme
0.003
Glutaraldehyde
-
mutant enzyme S74A
0.0062
Glutaraldehyde
-
isoenzyme ALDH-II
0.05
Glutaraldehyde
-
isoenzyme ALDH-I
0.56
Glutaraldehyde
-
membrane-bound isoenzyme ALDH
0.17
glyceraldehyde
-
D-glyceraldehyde, L-glyceraldehyde, DL-glyceraldehyde
0.595
glyceraldehyde
-
wild type isoform ALDH1A1, in 100 mM sodium diphosphate (pH 9.5), at 25°C
7.374
glyceraldehyde
-
pH and temperature not specified in the publication
0.097
glyceraldehyde 3-phosphate
wild type enzyme, at pH 7.5 and 30°C
0.372
glyceraldehyde 3-phosphate
-
-
0.005
glycolaldehyde
-
isoenzyme I
0.021
glycolaldehyde
-
isoenzyme II
0.038
glycolaldehyde
-
native enzyme
0.077
glycolaldehyde
-
mutant enzyme S74A
0.11
glycolaldehyde
-
isoenzyme III
0.2
glycolaldehyde
-
enzyme from brain
0.24
glycolaldehyde
-
enzyme from liver
0.000018
Heptanal
-
enzyme form ALDH-1, pH 9.5
0.000027
Heptanal
-
enzyme form ALDH-2, pH 9.5
0.035
Heptanal
-
microsomal enzyme
0.00003
hexanal
-
enzyme form ALDH-2, pH 9.5
0.000041
hexanal
-
enzyme form ALDH-1, pH 9.5
0.0004
hexanal
-
at 25°C, pH not specified in the publication
0.0008
hexanal
-
native enzyme
0.0018
hexanal
1 mM NAD+, 0,05 mM substrate, 250 mM sodium bicarbonate-NaOH buffer pH 10.0, 37°C
0.0024
hexanal
-
mutant enzyme S74A
0.0035
hexanal
-
pH 8.0, 25°C
0.0134
hexanal
-
pH 8.0, 25°C
0.0213
hexanal
-
pH 8.0, 22°C, recombinant wild-type enzyme
0.0391
hexanal
-
pH and temperature not specified in the publication
0.071
hexanal
wild type enzyme, at pH 8.0 and 25°C
0.071
hexanal
-
in 100 mM sodium diphosphate, at pH 8.0 and 22°C
0.072
hexanal
mutant enzyme E149T/V178R/I200V, at pH 8.0 and 25°C
0.0007
indole 3-acetaldehyde
-
isoenzyme I
0.001
indole 3-acetaldehyde
-
isoenzyme III
0.00015
Indole-3-acetaldehyde
-
enzyme ALDH-2
0.00031
Indole-3-acetaldehyde
-
enzyme ALDH-1
0.002
Indole-3-acetaldehyde
-
-
0.03
Isobutanal
-
-
0.21
Isovaleraldehyde
-
recombinant enzyme, in 50 mM potassium phosphate buffer, at pH 8.0 and 45°C
0.68
Isovaleraldehyde
-
with NAD+ as cofactor, in 50 mM potassium phosphate buffer (pH 8.0), at 37°C
0.0043
malondialdehyde
-
wild type isoform ALDH1A1, in 100 mM sodium diphosphate (pH 9.5), at 25°C
0.014
malondialdehyde
-
pH 8.0, 25°C
0.1144
malondialdehyde
-
pH 8.0, 25°C
0.466
malondialdehyde
-
at 25°C, pH not specified in the publication
0.0086
methylglyoxal
-
pH 7.4, isoenzyme E2
0.021
methylglyoxal
-
pH 9.0, isoenzyme E2
0.552
methylglyoxal
-
minor component of enzyme form E3, pH 7.4
0.586
methylglyoxal
-
major component of enzyme form E3, pH 7.4
0.958
methylglyoxal
-
minor component of enzyme form E3, pH 9.0
1.876
methylglyoxal
-
major component of enzyme form E3, pH 9.0
0.0008
n-nonanal
-
at 25°C, pH not specified in the publication
0.0285
n-nonanal
-
pH and temperature not specified in the publication
0.0003
NAD+
50 mM sodium phosphate buffer, pH 7.4, 0.2 mM Mg2+,0.14 mM propanal, 25°C
0.0012
NAD+
-
apparent Km value
0.0017
NAD+
-
pH 7.5, 25°C, mutant enzyme S82A
0.0019
NAD+
-
reaction with propanal
0.0023
NAD+
-
enzyme from promotion phase of hepatocarcinogenesis
0.0026
NAD+
-
reaction with butanal
0.003
NAD+
-
reaction with propanal
0.003
NAD+
-
isoenzyme F1
0.0035
NAD+
-
pH 7.5, 25°C, mutant enzyme D80G
0.0036
NAD+
50 mM sodium phosphate buffer, pH 7.4, 0.25 mM Mg2+,0.14 mM propanal, 25°C
0.0036
NAD+
-
at 25°C, pH not specified in the publication
0.004
NAD+
-
brain enzyme
0.004
NAD+
-
native enzyme, pH 7.4
0.004
NAD+
-
pH 7.5, 25°C, mutant enzyme R84Q
0.005
NAD+
-
isoenzyme ALDH1
0.0062
NAD+
50 mM sodium phosphate buffer, pH 7.4, 0.14 mM propanal, 25°C
0.0077
NAD+
-
phenobarbital-inducible enzyme
0.008
NAD+
-
isoenzyme 1, pH 9.5
0.008
NAD+
-
pH 7.5, 25°C, mutant enzyme ALDH1-5AA
0.01
NAD+
-
native enzyme, reaction with propanal
0.011
NAD+
-
pH 7.5, 25°C, wild-type enzyme
0.011
NAD+
-
wild type isoform ALDH1A1, in 100 mM sodium diphosphate (pH 9.5), at 25°C
0.0125
NAD+
-
cytoplasmic isoenzyme
0.014
NAD+
-
enzyme from liver
0.014 - 0.015
NAD+
-
reaction with 4-aminobutyraldehyde
0.0145
NAD+
50 mM sodium phosphate buffer, pH 7.4, 0.14 mM propanal, 25°C
0.016
NAD+
-
isoenzyme ALDH2
0.018
NAD+
mutant enzyme R108A, with NAD+ in 50 mM HEPES, pH 7.5 supplemented with 10 mM MgSO4 for 5 min at 30°C
0.018
NAD+
mutant enzyme R108A, with phosphonoacetaldehyde as cosubstrate, at pH 7.5 and 30°C
0.022
NAD+
-
reaction with propanal, enzyme II from mitochondria
0.022
NAD+
-
pH 7.5, 25°C, mutant enzyme ALDH1-H3tail
0.03
NAD+
-
isoenzyme ALDH-II
0.03
NAD+
-
in the presence of 0.01 mM Alda-1, at pH 7.4 and 25°C
0.036
NAD+
-
reaction with acetaldehyde
0.036
NAD+
-
reaction with propanal, enzyme I from mitochondria
0.0367
NAD+
-
reaction with acetaldehyde, at pH 9.5
0.037
NAD+
-
native enzyme
0.038
NAD+
-
reaction with butanal
0.038
NAD+
-
isoenzyme ALDH-I
0.0383
NAD+
-
reaction with acetaldehyde, at pH 7.5
0.04
NAD+
-
isoenzyme 1, pH 7.0
0.04
NAD+
mutant enzyme N158A, with NAD+ in 50 mM HEPES, pH 7.5 supplemented with 10 mM MgSO4 for 5 min at 30°C
0.04
NAD+
mutant enzyme N158A, with phosphonoacetaldehyde as cosubstrate, at pH 7.5 and 30°C
0.041
NAD+
-
enzyme from phenotype Aldh2(1)/Aldh2(3)
0.041
NAD+
-
enzyme from phenotype Aldh2(3)/Aldh2(3)
0.041
NAD+
50 mM sodium phosphate buffer, pH 7.4, 0.014 mM propanal, 25°C
0.043
NAD+
-
enzyme from phenotype Aldh2(1)/Aldh2(1)
0.044
NAD+
-
reaction with butanal
0.047
NAD+
-
100 mM Na2HPO4, pH 7.4, 100 mM NaCl, 1 mM NAD+, 25°C
0.054
NAD+
mutant enzyme R447A, with NAD+ in 50 mM HEPES, pH 7.5 supplemented with 10 mM MgSO4 for 5 min at 30°C
0.054
NAD+
mutant enzyme R447A, with phosphonoacetaldehyde as cosubstrate, at pH 7.5 and 30°C
0.058
NAD+
wild type enzyme, with NAD+ in 50 mM HEPES, pH 7.5 supplemented with 10 mM MgSO4 for 5 min at 30°C
0.058
NAD+
wild type enzyme, with phosphonoacetaldehyde as cosubstrate, at pH 7.5 and 30°C
0.06
NAD+
-
mutant enzyme R264Q
0.07
NAD+
-
native enzyme, pH 7.4
0.07
NAD+
-
isoenzyme 2, pH 7.0 and pH 9.5
0.073
NAD+
-
in the absence of Alda-1, at pH 7.4 and 25°C
0.074
NAD+
-
native enzyme, pH 9.0
0.075
NAD+
mutant enzyme R290A, with NAD+ in 50 mM HEPES, pH 7.5 supplemented with 10 mM MgSO4 for 5 min at 30°C
0.075
NAD+
mutant enzyme R290A, with phosphonoacetaldehyde as cosubstrate, at pH 7.5 and 30°C
0.077
NAD+
-
mitochondrial isoenzyme
0.09
NAD+
-
mutant enzyme E487Q
0.09
NAD+
0,05 mM propanal, 250 mM sodium bicarbonate-NaOH buffer pH 10.0, 37°C
0.09
NAD+
-
recombinant enzyme, in 50 mM potassium phosphate buffer, at pH 8.0 and 45°C
0.095
NAD+
-
reaction with propanal, enzyme II from microsomes
0.097
NAD+
-
reaction with propanal, enzyme I from microsomes
0.119
NAD+
-
wild type enzyme, with nonanal as cosubstrate, in 100 mM sodium diphosphate, at pH 8.0 and 22°C
0.12
NAD+
50 mM sodium phosphate buffer, pH 7.4, 0.2 mM Mg2+,0.014 mM propanal, 25°C
0.132
NAD+
-
enzyme from phenotype Aldh2(2)/Aldh2(2)
0.136
NAD+
-
mutant S82A/D80G of isoform ALDH1A1, in 100 mM sodium diphosphate (pH 9.5), at 25°C
0.2
NAD+
-
reaction with decanal
0.37
NAD+
mutant enzyme E385A, with NAD+ in 50 mM HEPES, pH 7.5 supplemented with 10 mM MgSO4 for 5 min at 30°C
0.37
NAD+
mutant enzyme E385A, with phosphonoacetaldehyde as cosubstrate, at pH 7.5 and 30°C
0.389
NAD+
-
in 50 mM KH2PO4-K2HPO4 buffer (pH 8.0), at 60°C
0.4
NAD+
-
pH 9.5, reaction with acetaldehyde
0.421
NAD+
-
wild type enzyme, with hexanal as cosubstrate, in 100 mM sodium diphosphate, at pH 8.0 and 22°C
0.421
NAD+
wild type enzyme, with hexanal as cosubstrate, at pH 8.0 and 25°C
0.441
NAD+
mutant enzyme E149D, with hexanal as cosubstrate, at pH 8.0 and 25°C
0.496
NAD+
-
mutant enzyme I200V, with hexanal as cosubstrate, in 100 mM sodium diphosphate, at pH 8.0 and 22°C
0.496
NAD+
mutant enzyme I200V, with hexanal as cosubstrate, at pH 8.0 and 25°C
0.5
NAD+
-
at pH 7.7 and 25°C
0.53
NAD+
wild type enzyme, with glyceraldehyde-3-phosphate as cosubstrate, at pH 7.5 and 30°C
0.564
NAD+
mutant enzyme E149T/I200V, with trans-2-nonenal as cosubstrate, at pH 8.0 and 25°C
0.62
NAD+
-
reaction with formaldehyde
0.63
NAD+
mutant enzyme E149N, with hexanal as cosubstrate, at pH 8.0 and 25°C
0.7
NAD+
-
mutant enzyme E487K, pH 7.4
0.74
NAD+
-
mutant enzyme R274E
0.82
NAD+
-
with Dl-glyceraldehyde as cosubstrate, in 50 mM Tris/HCl, at pH 8.0 and 80°C
0.84
NAD+
-
with acetaldehyde as cosubstrate, in 50 mM Tris/HCl, at pH 8.0 and 80°C
0.85
NAD+
-
mutant enzyme R475Q
0.886
NAD+
mutant enzyme E149Q, with hexanal as cosubstrate, at pH 8.0 and 25°C
0.991
NAD+
mutant enzyme E149D/I200V, with hexanal as cosubstrate, at pH 8.0 and 25°C
0.992
NAD+
mutant enzyme E149N/I200V, with hexanal as cosubstrate, at pH 8.0 and 25°C
1
NAD+
-
at pH 7.5 and 30°C
1.068
NAD+
mutant enzyme E149T/V178R/I200V, with trans-2-nonenal as cosubstrate, at pH 8.0 and 25°C
1.092
NAD+
mutant enzyme E149T/I200V, with trans-2-hexenal as cosubstrate, at pH 8.0 and 25°C
1.1
NAD+
-
mutant enzyme E487K, pH 9.0
1.262
NAD+
mutant enzyme E149T, with hexanal as cosubstrate, at pH 8.0 and 25°C
1.3
NAD+
-
mutant enzyme R475Q/R264Q and R475E
1.622
NAD+
mutant enzyme E149T/V178R/I200V, with trans-2-hexenal as cosubstrate, at pH 8.0 and 25°C
1.648
NAD+
mutant enzyme E149T/I200V, with hexanal as cosubstrate, at pH 8.0 and 25°C
1.649
NAD+
mutant enzyme E149T/I200V, with hexanal as cosubstrate, at pH 8.0 and 25°C
1.829
NAD+
mutant enzyme E149T/I200V, with nonanal as cosubstrate, at pH 8.0 and 25°C
1.856
NAD+
mutant enzyme E149Q/I200V, with hexanal as cosubstrate, at pH 8.0 and 25°C
1.9
NAD+
-
mutant enzyme S74A, reaction with propanal
2.115
NAD+
mutant enzyme E149T/V178R/I200V, with nonanal as cosubstrate, at pH 8.0 and 25°C
2.524
NAD+
mutant enzyme E149T/V178R/I200V, with hexanal as cosubstrate, at pH 8.0 and 25°C
3.218
NAD+
-
mutant enzyme I200G, with hexanal as cosubstrate, in 100 mM sodium diphosphate, at pH 8.0 and 22°C
3.218
NAD+
mutant enzyme I200G, with hexanal as cosubstrate, at pH 8.0 and 25°C
3.418
NAD+
mutant enzyme E149T/I200G, with hexanal as cosubstrate, at pH 8.0 and 25°C
3.651
NAD+
mutant enzyme E149D/I200G, with hexanal as cosubstrate, at pH 8.0 and 25°C
4.059
NAD+
mutant enzyme E149Q/I200G, with hexanal as cosubstrate, at pH 8.0 and 25°C
4.136
NAD+
mutant enzyme E149N/I200G, with hexanal as cosubstrate, at pH 8.0 and 25°C
7.4
NAD+
-
mutant enzyme E487K
16
NAD+
-
mutant enzyme R475E/R264E
0.27
NADP+
-
reaction with benzaldehyde, enzyme II from microsomes
0.44
NADP+
-
reaction with benzaldehyde, enzyme II from mitochondria
0.44
NADP+
-
reaction with benzaldehyde, enzyme I from microsomes
0.91
NADP+
-
reaction with benzaldehyde, enzyme I from mitochondria
3.81
NADP+
-
with acetaldehyde as cosubstrate, in 50 mM Tris/HCl, at pH 8.0 and 80°C
8.47
NADP+
-
with Dl-glyceraldehyde as cosubstrate, in 50 mM Tris/HCl, at pH 8.0 and 80°C
0.008
Nonanal
wild type enzyme, at pH 8.0 and 25°C
0.008
Nonanal
-
in 100 mM sodium diphosphate, at pH 8.0 and 22°C
0.065
Nonanal
mutant enzyme E149T/V178R/I200V, at pH 8.0 and 25°C
0.000012
octanal
-
enzyme form ALDH-1, pH 9.5
0.000028
octanal
-
enzyme form ALDH-2, pH 9.5
0.000085
octanal
1 mM NAD+, 0,05 mM substrate, 250 mM sodium bicarbonate-NaOH buffer pH 10.0, 37°C
0.0175
octanal
-
pH and temperature not specified in the publication
0.029
octanal
-
in 100 mM sodium diphosphate, at pH 8.0 and 22°C
0.000005
p-(dimethylamino)cinnamaldehyde
-
enzyme ALDH-2
0.0009
p-(dimethylamino)cinnamaldehyde
-
enzyme ALDH-1
0.00142
p-(dimethylamino)cinnamaldehyde
-
enzyme ALDH-1
0.000018
p-Methoxybenzaldehyde
-
enzyme ALDH-2
0.0001
p-Methoxybenzaldehyde
-
native enzyme
0.0004
p-Methoxybenzaldehyde
-
mutant enzyme S74A
0.000007
p-nitrobenzaldehyde
-
enzyme ALDH-2
0.0001
p-nitrobenzaldehyde
-
native enzyme
0.0004
p-nitrobenzaldehyde
-
mutant enzyme S74A
0.0034
p-nitrobenzaldehyde
-
-
0.018
p-nitrobenzaldehyde
-
isoenzyme ALDH-II
0.056
p-nitrobenzaldehyde
-
membrane-bound isoenzyme ALDH
2
p-nitrobenzaldehyde
-
isoenzyme ALDH-I
0.000034
pentanal
-
enzyme form ALDH-2, pH 9.5
0.00016
pentanal
-
enzyme form ALDH-1, pH 9.5
0.000029
phenylacetaldehyde
-
enzyme ALDH-2
0.00352
phenylacetaldehyde
-
-
0.0055
phenylacetaldehyde
-
enzyme ALDH-1
0.02
phenylacetaldehyde
-
at pH 7.7 and 25°C
0.027
phenylacetaldehyde
-
-
0.0032
phosphonoacetaldehyde
wild type enzyme, at pH 7.5 and 30°C
0.0032
phosphonoacetaldehyde
wild type enzyme, with NAD+ in 50 mM HEPES, pH 7.5 supplemented with 10 mM MgSO4 for 5 min at 30°C
0.005
phosphonoacetaldehyde
mutant enzyme R290A, with NAD+ in 50 mM HEPES, pH 7.5 supplemented with 10 mM MgSO4 for 5 min at 30°C
0.0051
phosphonoacetaldehyde
mutant enzyme R290A, at pH 7.5 and 30°C
0.009
phosphonoacetaldehyde
mutant enzyme R108A, with NAD+ in 50 mM HEPES, pH 7.5 supplemented with 10 mM MgSO4 for 5 min at 30°C
0.0097
phosphonoacetaldehyde
mutant enzyme R108A, at pH 7.5 and 30°C
0.019
phosphonoacetaldehyde
mutant enzyme E385A, with NAD+ in 50 mM HEPES, pH 7.5 supplemented with 10 mM MgSO4 for 5 min at 30°C
0.019
phosphonoacetaldehyde
mutant enzyme E385A, at pH 7.5 and 30°C
0.029
phosphonoacetaldehyde
mutant enzyme N158A, with NAD+ in 50 mM HEPES, pH 7.5 supplemented with 10 mM MgSO4 for 5 min at 30°C
0.029
phosphonoacetaldehyde
mutant enzyme N158A, at pH 7.5 and 30°C
0.15
phosphonoacetaldehyde
mutant enzyme R447A, with NAD+ in 50 mM HEPES, pH 7.5 supplemented with 10 mM MgSO4 for 5 min at 30°C
0.15
phosphonoacetaldehyde
mutant enzyme R447A, at pH 7.5 and 30°C
0.5
phosphonoacetaldehyde
-
at pH 7.5 and 30°C
0.000008
propanal
-
major component of enzyme E3
0.00001
propanal
-
minor component of enzyme E3
0.00004
propanal
-
minor component of enzyme E3
0.00008
propanal
-
native enzyme, pH 7.4
0.000095
propanal
-
enzyme form ALDH-2, pH 9.5
0.0001
propanal
-
native enzyme, pH 9.0
0.00011
propanal
-
pH 7.5
0.0002
propanal
-
mutant enzyme E487Q, pH 7.4
0.0002
propanal
50 mM sodium phosphate buffer, pH 7.4, 0.2 mM Mg2+,1 mM NAD+, 25°C
0.0002
propanal
50 mM sodium phosphate buffer, pH 7.4, 1 mM NAD+, 25°C
0.00035
propanal
-
pH 9.5
0.0006
propanal
-
mutant enzyme E487K, pH 7.4
0.0007
propanal
-
native enzyme
0.0007
propanal
-
enzyme form ALDH-1, pH 9.5
0.0007
propanal
-
native enzyme, pH 7.4
0.0007
propanal
-
isoenzyme 2, pH 7.0
0.0007
propanal
-
in the absence of Alda-1, at pH 7.4 and 25°C
0.0008
propanal
-
native enzyme and mutant enzyme S74A
0.0009
propanal
-
mutant enzyme S74A
0.0012
propanal
-
isoenzyme 2, pH 9.5
0.0012
propanal
50 mM sodium phosphate buffer, pH 7.4, 0.2 mM Mg2+,1 mM NAD+, 25°C
0.0014
propanal
-
mutant enzyme E487K, pH 7.4
0.0017
propanal
-
mutant enzyme E487K, pH 9.0
0.0024
propanal
0.1 M sodium diphosphate pH 8.0, 1 mM NAD+, 50 mM 2-mercaptoethanol, 1 mM pyrazole, 30 min, 25°C
0.0033
propanal
-
isoenzyme ALDH-II
0.0034
propanal
50 mM sodium phosphate buffer, pH 7.4, 0.25 mM Mg2+,1 mM NAD+, 25°C
0.004
propanal
-
isoenzyme III
0.0045
propanal
-
enzyme form ALDH-1, pH 9.5
0.0052
propanal
50 mM sodium phosphate buffer, pH 7.4, 1 mM NAD+, 25°C
0.0053
propanal
-
100 mM Na2HPO4, pH 7.4, 100 mM NaCl, 1 mM NAD+, 25°C
0.0058
propanal
50 mM sodium phosphate buffer, pH 7.4, 1 mM NAD+, 25°C
0.006
propanal
-
in the presence of 0.01 mM Alda-1, at pH 7.4 and 25°C
0.0061
propanal
-
0.1 M sodium diphosphate pH 8.0, 1 mM NAD+, 50 mM 2-mercaptoethanol, 1 mM pyrazole, 30 min, 25°C
0.0091
propanal
1 mM NAD+, 0,05 mM substrate, 250 mM sodium bicarbonate-NaOH buffer pH 10.0, 37°C
0.011
propanal
-
isoenzyme 2, pH 9.5
0.15
propanal
-
enzyme I and II from mitochondria
0.294
propanal
-
microsomal enzyme
0.5
propanal
-
isoenzyme ALDH-I
0.6474
propanal
-
pH and temperature not specified in the publication
0.765
propanal
-
at substrate concentrations 0.8-20 mM
1
propanal
-
isoenzyme 1, pH 7.0
1.3
propanal
-
enzyme from promotion phase of hepatocarcinogenesis
1.4
propanal
-
phenobarbital-inducible enzyme
1.7
propanal
-
enzyme II from microsome
2
propanal
-
membrane-bound isoenzyme ALDH
2.76
propanal
pH 8.0, 50°C, recombinant wild-type enzyme
0.0028
propionaldehyde
-
pH 7.5, 25°C, mutant enzyme D80G
0.0044
propionaldehyde
-
pH 7.5, 25°C, mutant enzyme S82A
0.008
propionaldehyde
-
pH 7.5, 25°C, mutant enzyme ALDH1-5AA
0.012
propionaldehyde
-
pH 7.5, 25°C, wild-type enzyme
0.012
propionaldehyde
-
wild type isoform ALDH1A1, in 100 mM sodium diphosphate (pH 9.5), at 25°C
0.014
propionaldehyde
-
at 25°C, pH not specified in the publication
0.031
propionaldehyde
-
pH 7.5, 25°C, mutant enzyme ALDH1-H3tail
0.0662
propionaldehyde
-
pH 8.0, 25°C
0.07
propionaldehyde
-
pH 7.5, 25°C, mutant enzyme R84Q
0.1372
propionaldehyde
-
pH 8.0, 25°C
0.189
propionaldehyde
-
mutant S82A/D80G of isoform ALDH1A1, in 100 mM sodium diphosphate (pH 9.5), at 25°C
0.51
propionaldehyde
-
in 100 mM sodium diphosphate, at pH 8.0 and 22°C
0.82
propionaldehyde
at pH 8.0 and 21°C
1.21
propionaldehyde
-
with NAD+ as cofactor, in 50 mM potassium phosphate buffer (pH 8.0), at 37°C
12
propionaldehyde
in 0.1 M sodium diphosphate buffer (pH 8.5), at ambient temperature
0.69
Succinic semialdehyde
+/- 0.22
3.54
Succinic semialdehyde
-
-
0.18
trans-2-hexenal
wild type enzyme, at pH 8.0 and 25°C
0.18
trans-2-hexenal
-
in 100 mM sodium diphosphate, at pH 8.0 and 22°C
0.199
trans-2-hexenal
mutant enzyme E149T/V178R/I200V, at pH 8.0 and 25°C
0.003
trans-2-nonenal
wild type enzyme, at pH 8.0 and 25°C
0.003
trans-2-nonenal
-
in 100 mM sodium diphosphate, at pH 8.0 and 22°C
0.0322
trans-2-nonenal
mutant enzyme E149T/V178R/I200V, at pH 8.0 and 25°C
0.000035
trans-cinnamaldehyde
-
enzyme ALDH-2
0.0004
trans-cinnamaldehyde
-
enzyme ALDH-1
0.14
Valeraldehyde
-
recombinant enzyme, in 50 mM potassium phosphate buffer, at pH 8.0 and 45°C
0.24
Valeraldehyde
-
with NAD+ as cofactor, in 50 mM potassium phosphate buffer (pH 8.0), at 37°C
additional information
additional information
-
influence of pH-value and buffer on Km-value for hexanal
-
additional information
additional information
-
Km-values of the 475 and 264 arginine mutants of Oriental variant E847K
-
additional information
additional information
-
Michaelis-Menten steady-state kinetics of wild-type and mutant enzymes
-
additional information
additional information
-
Michaelis-Menten kinetics and modeling
-