1.2.1.28: benzaldehyde dehydrogenase (NAD+)
This is an abbreviated version!
For detailed information about benzaldehyde dehydrogenase (NAD+), go to the full flat file.
Reaction
Synonyms
BALDH, benzaldehyde dehydrogenase, benzaldehyde dehydrogenase (NAD), BZDH, mdlD, More, NAD(P)-dependent benzaldehyde dehydrogenase, PpBADH, XylC
ECTree
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Cloned
Cloned on EC 1.2.1.28 - benzaldehyde dehydrogenase (NAD+)
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expression in Escherichia coli
gene mdlD, recombinant epression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
gene xylC, recombinant expression in Escherichia coli strain BL21(DE3) in inclusion bodies. Using higher concentration of lysozyme in lysis buffer, high speed, and long-term centrifugation reduces the protein aggregation
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into the vector pET-28a+ for expression in Escherichia coli BL21 Rosetta cells, into the pEF1-LIS binary vector for transformation of petunia flowers with Agrobacterium tumefaciens, for subcellular localization experiments in Arabidopsis protoplasts GFP fusion proteins are constructed using the vector p326-SGFP
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