1.2.1.27: methylmalonate-semialdehyde dehydrogenase (CoA-acylating)

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Word Map on EC 1.2.1.27

1.2.1.27

lysosomotropic

3-hydroxyisobutyrate

coa-dependent

mississippi

propionyl-coa

aldh6a1

3-hydroxypropionic

Reaction

Synonyms

Aldh6a1, dddC, FG99_15390, KES23460, malonate-semialdehyde dehydrogenase, methylmalonate semialdehyde dehydrogenase, methylmalonate semialdehyde dehydrogenase protein, methylmalonate-semialdehyde dehydrogenase, methylmalonic acid semialdehyde dehydrogenase, MGG_01606, MMSADH, MMSDH, MoMSDH, MSDH, NAD-dependent malonate-semialdehyde dehydrogenase, OdoMMSDH, OsALDH6, PA3570, SSO1218

ECTree

1 Oxidoreductases

1.2 Acting on the aldehyde or oxo group of donors

1.2.1 With NAD⁺ or NADP⁺ as acceptor

1.2.1.27 methylmalonate-semialdehyde dehydrogenase (CoA-acylating)

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Results	in table
10477	AA Sequence
1	CAS Registry Number
12	Cloned(Commentary)
28	Cofactor
4	Crystallization (Commentary)
25	Disease/ Diagnostics
3	Expression
22	General Information
9	Inhibitors
3	kcat/KM [mM/s]
1	Ki Value [mM]
55	KM Value [mM]
5	Localization
11	Molecular Weight [Da]
19	Natural Substrates/ Products (Substrates)
40	Organism
7	Pathway
28	PDB ID
7	pH Optimum
5	pH Range
1	pI Value
2	Posttranslational Modification
22	Protein Variants
13	Purification (Commentary)
1	Reaction
4	Reaction Type
30	Reference
17	Source Tissue
2	Specific Activity [micromol/min/mg]
3	Storage Stability
59	Substrates and Products (Substrate)
17	Subunits
53	Synonyms
1	Systematic Name
1	Temperature Optimum [°C]
20	Turnover Number [1/s]

Purification

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Purification on EC 1.2.1.27 - methylmalonate-semialdehyde dehydrogenase (CoA-acylating)

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4 entries

MSDH purified by ammonium sulfate fractionation, 40-80%, and gel filtration on a ACA 34 resin

2 entries

partial

Pseudomonas aeruginosa

390220

recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, gel filtration, and ultrafiltration to over 95% purity

recombinant MSDH

recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3). Among the twelve molecules in the asymmetric unit, six subunits complexed with NAD+

Oceanimonas doudoroffii

G5CZI2

743075

to apparent homogeneity using heat precipitation at 80°C for 20 min, ion exchange chromatography and gel filtration

Saccharolobus solfataricus

Q97YT9

722211