1.2.1.27: methylmalonate-semialdehyde dehydrogenase (CoA-acylating)

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For detailed information about methylmalonate-semialdehyde dehydrogenase (CoA-acylating), go to the full flat file.

Reaction

Synonyms

Aldh6a1, dddC, FG99_15390, KES23460, malonate-semialdehyde dehydrogenase, methylmalonate semialdehyde dehydrogenase, methylmalonate semialdehyde dehydrogenase protein, methylmalonate-semialdehyde dehydrogenase, methylmalonic acid semialdehyde dehydrogenase, MGG_01606, MMSADH, MMSDH, MoMSDH, MSDH, NAD-dependent malonate-semialdehyde dehydrogenase, OdoMMSDH, OsALDH6, PA3570, SSO1218

ECTree

     1 Oxidoreductases

         1.2 Acting on the aldehyde or oxo group of donors

             1.2.1 With NAD⁺ or NADP⁺ as acceptor

                1.2.1.27 methylmalonate-semialdehyde dehydrogenase (CoA-acylating)

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Results	in table
10477	AA Sequence
1	CAS Registry Number
12	Cloned(Commentary)
28	Cofactor
4	Crystallization (Commentary)
25	Disease/ Diagnostics
3	Expression
22	General Information
9	Inhibitors
3	kcat/KM [mM/s]
1	Ki Value [mM]
55	KM Value [mM]
5	Localization
11	Molecular Weight [Da]
19	Natural Substrates/ Products (Substrates)
40	Organism
7	Pathway
28	PDB ID
7	pH Optimum
5	pH Range
1	pI Value
2	Posttranslational Modification
22	Protein Variants
13	Purification (Commentary)
1	Reaction
4	Reaction Type
30	Reference
17	Source Tissue
2	Specific Activity [micromol/min/mg]
3	Storage Stability
59	Substrates and Products (Substrate)
17	Subunits
53	Synonyms
1	Systematic Name
1	Temperature Optimum [°C]
20	Turnover Number [1/s]

Crystallization

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Crystallization on EC 1.2.1.27 - methylmalonate-semialdehyde dehydrogenase (CoA-acylating)

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CRYSTALLIZATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

hanging-drop vapour diffusion, crystals diffract to 2.5 A resolution

Bacillus subtilis

-

654105

methylmalonate semialdehyde dehydrogenase in binary complex with NAD+, showing that the nicotinamide ring is well defined in the electron density due to direct and H2O-mediated hydrogen bonds with the carboxamide and that a conformational isomerization of the NMNH is possible in MSDH

Bacillus subtilis

P42412

725485

purified recombinant His6-tagged enzyme, mixing of 0.001 ml of protein solution containing 32.2 mg/ml protein in 20 mM Tris-HCl, pH 8.0, 150 mM NaCl, and 1 mM DTT, with 0.001 ml of reservoir solution consisting of 21% PEG 3350 and 0.2 M potassium sodium tartrate, pH 7.5, at 20°C, 2 days, X-ray diffraction sructure determination and analysis at 2.9 A resolution, Among the twelve molecules in the asymmetric unit, six subunits complexed with NAD+

Oceanimonas doudoroffii

G5CZI2

743075

purified recombinant His-tagged enzyme, microseeding, mixing of 150 nl of 5 mg/ml protein in 40 mM Tris Cl, 150 mM NaCl, 2 mM KCl, and TBS, pH 8, with 150 nl of reservoir solution containing 23.7% w/v PEG 3350, 0.208 M trisodium citrate, 0.1 M Bis-Tris propane, pH 7.55, and equilibration against 0.05 ml of reservoir solution, at 8°C, X-ray diffraction structure determination and analysis at 2.95 A resolution, molecular replacement and modeling using PDB entry 4zz7 as a template

Pseudomonas sp.

-

741539