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0.00041 - 5.8
3-phospho-D-glyceroyl phosphate
0.75 - 0.838
D-glyceraldehyde 3-phosphate
0.21 - 0.77
DL-glyceraldehyde 3-phosphate
additional information
additional information
-
0.00041
3-phospho-D-glyceroyl phosphate
pH 6.5, 70°C
0.01
3-phospho-D-glyceroyl phosphate
mutant enzyme T33A/S188A, reaction with NADH
0.012
3-phospho-D-glyceroyl phosphate
mutant enzyme S188A, reaction with NADH
0.012
3-phospho-D-glyceroyl phosphate
mutant enzyme T33A, reaction with NADH
0.012
3-phospho-D-glyceroyl phosphate
mutant enzyme T33A/S188A, reaction with NADPH
0.012
3-phospho-D-glyceroyl phosphate
native enzyme A3-GAPDH, reaction with NADH
0.012
3-phospho-D-glyceroyl phosphate
-
mutant B(E362Q)
0.013
3-phospho-D-glyceroyl phosphate
mutant enzyme S188A, reaction with NADPH
0.015
3-phospho-D-glyceroyl phosphate
native enzyme A3-GAPDH, reaction with NADPH
0.015
3-phospho-D-glyceroyl phosphate
-
GapA
0.015
3-phospho-D-glyceroyl phosphate
-
GapB, analyzed under reducing conditions
0.016
3-phospho-D-glyceroyl phosphate
-
with NADH as cofactor, activation by 20 mM dithiothreitol and 0.021 mM 1,3-bisphosphoglycerate
0.016
3-phospho-D-glyceroyl phosphate
-
mutant B(R77A)
0.018
3-phospho-D-glyceroyl phosphate
mutant enzyme T33A, reaction with NADPH
0.019
3-phospho-D-glyceroyl phosphate
-
A(plusCTE) mutant, analyzed under oxidizing conditions
0.02
3-phospho-D-glyceroyl phosphate
-
with NADPH as coenzyme, activation by 20 mM dithiothreitol and 0.021 mM 1,3-bisphosphoglycerate
0.02
3-phospho-D-glyceroyl phosphate
-
AB-GAPDH, analyzed under reducing conditions
0.02
3-phospho-D-glyceroyl phosphate
-
GapB, analyzed under oxidizing conditions
0.021
3-phospho-D-glyceroyl phosphate
-
A(plusCTE) mutant, analyzed under reducing conditions
0.022
3-phospho-D-glyceroyl phosphate
-
B(minCTE) mutant
0.024
3-phospho-D-glyceroyl phosphate
-
AB-GAPDH, analyzed under oxidizing conditions
0.027
3-phospho-D-glyceroyl phosphate
-
mutant B(188)A, analyzed under oxidizing conditions
0.031
3-phospho-D-glyceroyl phosphate
-
mutant B(188)A, analyzed under reducing conditions
5.8
3-phospho-D-glyceroyl phosphate
-
wild type enzyme, in 50 mM EPPS/NaOH (pH 8.0), 20 mM potassium phosphate, at 50°C
30
arsenate
-
-
0.75
D-glyceraldehyde 3-phosphate
pH 9.2, 30°C, reaction with NAD+ or NADP+
0.838
D-glyceraldehyde 3-phosphate
pH 6.5, 70°C
0.21
DL-glyceraldehyde 3-phosphate
-
wild type enzyme, with NADP+ as cosubstrate, in 50 mM EPPS/NaOH (pH 8.0), 20 mM potassium phosphate, at 60°C
0.77
DL-glyceraldehyde 3-phosphate
-
wild type enzyme, with NAD+ as cosubstrate, in 50 mM EPPS/NaOH (pH 8.0), 20 mM potassium phosphate, at 60°C
0.2
NAD+
-
-
16.8
NAD+
-
wild type enzyme, in 50 mM EPPS/NaOH (pH 8.0), 20 mM potassium phosphate, at 60°C
0.01
NADH
-
mutant enzyme R190A, pH and temperature not specified in the publication
0.099
NADH
-
mutant enzyme R197A
0.102
NADH
-
mutant enzyme R82D, pH and temperature not specified in the publication
0.11
NADH
-
mutant enzyme R197E
0.12
NADH
-
wild-type enzyme
0.12
NADH
-
pH 7.7, 30°C, recombinant A4 glyceraldehyde 3-phosphate dehydrogenase
0.12
NADH
-
wild type enzyme, pH and temperature not specified in the publication
0.136
NADH
-
pH 7.7, 30°C, native A4 glyceraldehyde 3-phosphate dehydrogenase
0.14
NADH
native enzyme A3-GAPDH
0.157
NADH
-
mutant enzyme R82A, pH and temperature not specified in the publication
0.168
NADH
mutant enzyme S188A
0.174
NADH
mutant enzyme T33A
0.184
NADH
mutant enzyme T33A/S188A
0.25
NADH
-
mutant enzyme K128A
0.272
NADH
-
mutant enzyme K128E
0.29
NADH
-
with NADH as coenzyme, activation by 20 mM dithiothreitol and 0.021 mM 1,3-bisphosphoglycerate
0.61
NADH
-
wild type enzyme, in 50 mM EPPS/NaOH (pH 8.0), 20 mM potassium phosphate, at 50°C
0.872
NADH
-
mutant enzyme S195A, pH and temperature not specified in the publication
0.003
NADP+
-
-
0.28
NADP+
-
wild type enzyme, in 50 mM EPPS/NaOH (pH 8.0), 20 mM potassium phosphate, at 60°C
0.002
NADPH
-
-
0.018
NADPH
-
pH 7.7, 30°C, native A4 glyceraldehyde 3-phosphate dehydrogenase
0.019
NADPH
-
B(minCTE) mutant
0.023
NADPH
-
mutant enzyme K128E
0.024
NADPH
-
mutant B(188)A, analyzed under oxidizing conditions
0.024
NADPH
-
mutant B(E362Q)
0.027
NADPH
-
mutant enzyme K128A
0.028
NADPH
-
wild-type enzyme
0.028
NADPH
-
mutant enzyme R197A
0.028
NADPH
-
pH 7.7, 30°C, recombinant A4 glyceraldehyde 3-phosphate dehydrogenase
0.028
NADPH
-
wild type enzyme, pH and temperature not specified in the publication
0.028
NADPH
-
mutant enzyme S195A, pH and temperature not specified in the publication
0.029
NADPH
native enzyme A3-GAPDH
0.03
NADPH
-
GapB, analyzed under reducing conditions
0.035
NADPH
-
mutant enzyme R197E
0.038
NADPH
-
GapB, analyzed under oxidizing conditions
0.043
NADPH
-
A(plusCTE) mutant, analyzed under oxidizing conditions
0.044
NADPH
mutant enzyme T33A
0.046
NADPH
-
mutant B(188)A, analyzed under reducing conditions
0.05
NADPH
mutant enzyme S188A
0.05
NADPH
mutant enzyme T33A/S188A
0.05
NADPH
-
AB-GAPDH, analyzed under oxidizing conditions
0.05
NADPH
-
AB-GAPDH, analyzed under reducing conditions
0.06
NADPH
-
activation by 20 mM dithiothreitol and 0.021 mM 1,3-bisphosphoglycerate
0.065
NADPH
-
A(plusCTE) mutant, analyzed under reducing conditions
0.078
NADPH
-
mutant B(R77A)
0.18
NADPH
-
wild type enzyme, in 50 mM EPPS/NaOH (pH 8.0), 20 mM potassium phosphate, at 50°C
0.202
NADPH
-
mutant enzyme R82D, pH and temperature not specified in the publication
0.3
NADPH
-
mutant enzyme R82A, pH and temperature not specified in the publication
10.7
phosphate
-
wild type enzyme, with NADP+ as cosubstrate, in 50 mM EPPS/NaOH (pH 8.0), 20 mM potassium phosphate, at 60°C
409
phosphate
pH 6.5, 70°C
additional information
additional information
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additional information
additional information
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-
additional information
additional information
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-
additional information
additional information
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-
additional information
additional information
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-
-
additional information
additional information
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no Michaelis-Menten kinetics with 1,3-bisphosphoglycerate
-