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1.2.1.12: glyceraldehyde-3-phosphate dehydrogenase (phosphorylating)

This is an abbreviated version!
For detailed information about glyceraldehyde-3-phosphate dehydrogenase (phosphorylating), go to the full flat file.

Word Map on EC 1.2.1.12

Reaction

D-glyceraldehyde 3-phosphate
+
phosphate
+
NAD+
=
3-phospho-D-glyceroyl phosphate
+
NADH
+
H+

Synonyms

3-phosphoglyceraldehyde dehydrogenase, A4-GAPDH, A4-glyceraldehyde-3-phosphate dehydrogenase, AB-GAPDH, AnBn-GAPDH, AsGAPDH, At3g04120, BARS-38, CbbG, CgGAP, Clo1313_2095, complement-C3-binding protein, CP 17/CP 18, Ctherm_Gapdh, cytosolic NAD-dependent glyceraldehyde 3-P dehydrogenase, cytosolic phosphorylating glyceraldehyde-3-phosphate dehydrogenase, D-glyceraldehyde-3-phosphate dehydrogenase, D-glyceraldehyde-3-phosphate: NAD+ oxidoreductase (phosphorylating), dehydrogenase, glyceraldehyde phosphate, dihydrogenase, glyceraldehyde phosphate, EcGAPDH, EcGAPDH1, FgGAPDH, FhGAPDH, G3PD, G3PDH, Ga3P dehydrogenase, Ga3PDHase, GADPH, GAP, GAP1, gap2, GapA, GapB, GAPC, GapC-1, GapC1, GapC2, GAPCp, GAPCp1, GAPCp2, GAPD, GAPDH, GAPDH type 1, GAPDH1, GAPDH2, GAPDH3, GAPDHS, GAPDS, GAPN, GBS GAPDH, glyceraldehyde 3-phosphate dehydrogenase, glyceraldehyde 3-phosphate dehydrogenase-S, glyceraldehyde phosphate dehydrogenase (NAD), glyceraldehyde-3 phosphate dehydrogenase, glyceraldehyde-3-P-dehydrogenase, glyceraldehyde-3-phosphate dehydrogenase, glyceraldehyde-3-phosphate dehydrogenase (NAD), glyceraldehyde-3-phosphate dehydrogenase 1, glyceraldehyde-3-phosphate dehydrogenase, type I, glyceraldehyde-3-phosphate dehydrogenase-spermatogenic protein, glyceraldehyde-3-phosphate dehydrogenase-spermatogenic protein GAPDHS, glyceraldehyde-3-phosphate dehydrogenases, GPD, GPD2, Gra3PDH, GraP-DH, H.c-C3BP, hGAPDH, HsGAPDH, kmGAPDH1p, Larval antigen OVB95, Major larval surface antigen, Mtb-GAPDH, NAD+-dependent GAPDH, NAD+-dependent glyceraldehyde 3-phosphate dehydrogenase, NAD+-dependent glyceraldehyde-3-phosphate dehydrogenase, NAD+-G-3-P dehydrogenase, NAD+-GAPDH, NAD-dependent Ga3PDHase, NAD-dependent glyceraldehyde 3-phosphate dehydrogenase, NAD-dependent glyceraldehyde phosphate dehydrogenase, NAD-dependent glyceraldehyde-3-phosphate dehydrogenase, NAD-dependent non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase, NAD-dependent phosphorylating glyceraldehyde-3-phosphate dehydrogenase, NAD-G3PDH, NAD-GAPDH, NADH-glyceraldehyde phosphate dehydrogenase, P-37, p-GAPDH, PfGAPDH, phosphoglyceraldehyde dehydrogenase, phosphorylating NAD+-dependent GAPDH, Plasmin receptor, Plasminogen-binding protein, plastidial glyceraldehyde-3-phosphate dehydrogenase, pmGAPDH, PyGapdh, rmGAPDH, Rv1436, somatic GAPD, somatic glyceraldehyde 3-phosphate dehydrogenase, sperm-specific GAPDS, sperm-specific glyceraldehyde 3-phosphate dehydrogenase, sperm-specific glyceraldehyde-3-phosphate dehydrogenase, TaeNAD-GAPDH, TagapC, TDH1, TDH2, TDH3, TLAb, triose phosphate dehydrogenase, UDG, uracil-DNA glycosylase, vGPD

ECTree

     1 Oxidoreductases
         1.2 Acting on the aldehyde or oxo group of donors
             1.2.1 With NAD+ or NADP+ as acceptor
                1.2.1.12 glyceraldehyde-3-phosphate dehydrogenase (phosphorylating)

Purification

Purification on EC 1.2.1.12 - glyceraldehyde-3-phosphate dehydrogenase (phosphorylating)

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
4 enzyme forms: E6.6, E6.8, E8.5 and E9.0
-
active-site CNBr peptide
-
affinity column chromatography and Sephadex G-100 gel filtration
-
ammonium sulfate fractionation and Blue Sepharose column chromatography
ammonium sulfate precipitation and DEAE-cellulose DE-52 column chromatography
-
ammonium sulfate precipitation and Q-Sepharose column chromatography
ammonium sulfate precipitation, DEAE-cellulose DE-52 column chromatography, and Cibacron Blue Sepharose column chromatography
-
analytical centrifugation
by affinity chromatography on a Hi-Trap chelating column charged with nickel sulfate, the His tag is cleaved and the enzyme is purified by an additional gel filtration step
cytosolic isoenzyme
-
DEAE-Sepharose column chromatography, ammonium sulfate precipitation, phenyl-Sepharose 6 column chromatography, and Superose 6 gel filtration
-
DEAE-Toyopearl column chromatography and hydroxyapatite column chromatography
-
extracellular serum isozyme by immunoaffinity purification combined with anion exchange chromatography by using DEAE affigel blue chromatography
from HeLa cells
from normal leukocytes of healthy subjects, leukocytes of chronic myeloid leukemia patients and from sarcoma tissue
-
GAPDH is subjected to gel filtration on a Sephadex G-100 column
-
glutathione Sepharose 4B resin column chromatography
-
GST-fusion proteins are expressed in Escherichia coli and subsequently purified by absorption to glutathione-Sepharose beads, GST is seperated by digestion with thrombin protease
-
high-affinity cAMP-binding protein I and II
-
histidine fusion protein in Escherichia coli TOP 10 strain
hydrophobic chromatography on immobilized colchicine
-
isoenzyme 1 and 2
-
isoenzyme TLAb-1 and TLAb-2
-
large-scale purification
-
mutant enzyme C149selenocysteine
-
NAD affinity chromatography
-
native enzyme 142-175fold by ultracentrifugation, ammonium sulfate fractionation, hydrophobic interaction chromatography, ultrafiltration, and gel filtration
-
native enzyme 430fold by affinity and hydrophobic interaction chromatography
-
native enzyme from bovine retinas using NAD+-agarose affinity chromatography
native enzyme from HeLa cells by ammonium sulfate fractionation followed by the heparin affinity chromatography, anion exchange chromatgraphy, and gel filtration
native GAPDH is purified from spinach chloroplasts
-
Ni-NTA agarose resin chromatography and Hi-Load 16/60 Superdex gel filtration
Ni-NTA column chromatography
Ni-NTA column chromatography, SP Sepharose column chromatography, and Superdex 200 gel filtration
Ni-Sepharose column chromatography and Superdex 75 gel filtration
Ni-Sepharose column chromatography and Superdex S75 gel filtration
Ni2+-NTA affinity column chromatography
Q81X74, YP_027084
Ni2+-NTA agarose resin chromatography
-
phenyl Sepharose column chromatography
purification of the secreted protein from the secretome of iron-starved cells by heme affinity chromatography with enzyme elution through a 6 M guanidine hydrochloride solution
purification protocol comprises two anion-exchange chromatographic steps and removing of the His tag
-
purified on a Ni-nitrilotriacetic acid resin column
purified on Superflow NTA nickel resin and gel chromatography, Superdex 75 26/60
radiommunoprecipitation with protein agarose A/G bead
-
recombinant GST-tagged GAPDH from gapA-deficient Escherichia coli strain DS112 by glutathione affinity chromatography, and on-column cleavage of the GST-tag by bovine thrombin before elution of the enzyme
recombinant GST-tagged tGAPDHS from gapA-deficient Escherichia coli strain DS112 by glutathione affinity chromatography, and on-column cleavage of the GST-tag by bovine thrombin before elution of the enzyme
recombinant His-tagged engineered enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, gel filtration, and ultrafiltration
recombinant His-tagged enzyme from Escherichia coli strain BL21 Codon PlusR (DE3) RIL by nickel affinity chromatography
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, dialysis, and ultrafiltration
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, gel filtration, and ultrafiltratioon
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickell affinity chromatography, dialysis, and gel filtration
-
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) CodonPlus RIPL by nickel affinity chormatography, His-tag cleavage by TEV protease
recombinant His-tagged enzyme from Escherichia coli strain DH5alpha by nickel affinity chromatography and dialysis, to homogeneity
recombinant His-tagged enzyme from Escherichia coli strain Rosetta(DE3) by nickel affinity chromatography
recombinant His-tagged wild-type and mutant enzymes from Mycobacterium tuberculosis strain H37Ra cell cytosol by nickel affinity chromatography
recombinant His6-tagged enzyme 2.8fold from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant MBP-tagged tGAPDHS from gapA-deficient Escherichia coli strain DS112 by amylose affinity chromatography, followed by cleavage of the MBP-tag by bovine thrombin dialysis, and anion exchange chromatography
recombinant N-terminally His6-tagged wild-type and mutant enzymes from Escherichia coli by nickel affinity chromatography and dialysis to over 965% purity
recombinant protein is purified using glutathione-Sepharose 4B and PreScission protease
recombinant tagged wild-type and mutant enzymes from Escherichia coli strain Rosetta(DE3)pLysS by nickel affinity chromatography and gel filtration
recombinant truncated mutant dN-GAPDS by cation exchange chromatography from Escherichia coli strain BL21(DE3). Recombinant enzyme mutants P197A and P164A by ammonium sulfate fractionation and dialysis, followed by anion exchange chromatography, the flow-through is further purified by hydrophobic interaction chromatography
recombinant untagged enzyme partially from Escherichia coli strain Rosetta 2 (DE3) by ammonium sulfate fractionation and precipitation in cyrstals, followed by desalting gel filtration, to homogeneity, method evaluation
recombinant wild-type and truncated mutant dN-GAPDS by cation exchange chromatography from Escherichia coli strain BL21(DE3), mutant dNGAPDS D311N by ammonium sulfate fractionation, and dialysis, followed by anion exchange chromatography, the flow-through is further purified by hydrophobic interaction chromatography
recombinant wild-type enzyme from Escherichia coli strain BL21(DE3)
red blood cell cytosol is prepared by hypotonic shock or freeze-thawing cycles, membranes are prepared by hypotonic lysis
-
the filtered supernatant of the centrifuged cell lysate is applied onto 3 ml Talon resin, the His-tag is removed, further purification by gel filtration
-
use of glyceraldehyde-3-phosphate dehydrogenase-depleted human erythrocyte ghosts as specific high affinity adsorbents for the purification
-
use of immunoaffinity column chromatography
-
using a nickel-nitrilo-triacetic acid-agarose column
-
using gel filtration on a AcA-34 column, and a Q-Sepharose and phenyl-Sepharose column
-
using Ni-NTA resin and a HiTrap Blue column, the His tag is removed
-