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1.18.1.3: ferredoxin-NAD+ reductase

This is an abbreviated version!
For detailed information about ferredoxin-NAD+ reductase, go to the full flat file.

Word Map on EC 1.18.1.3

Reaction

reduced ferredoxin
+
NAD+
+
H+
=
oxidized ferredoxin
+
NADH

Synonyms

BphA4, FdR, Fdx-FdR, ferredoxin NADPH reductase, ferredoxin-linked NAD reductase, ferredoxin-NAD reductase, ferredoxin-NAD(P)H reductase, ferredoxin-NAD+ reductase, ferredoxin-NADH oxidoreductase, ferredoxin-reductase, ferredoxin/flavodoxin-NAD+ reductase, More, NAD-ferredocinTOL reductase, NAD-ferredoxin reductase, NADH flavodoxin oxidoreductase, NADH-dependent ferredoxin reductase, NADH-ferredoxin oxidoreductase, NADH-ferredoxin reductase, NADH-ferredoxinNAP reductase, NADH2-ferredoxin oxidoreductase, palustrisredoxin reductase, PuR, Red, RedIIA, reductase, ferredoxin, reductase, ferredoxin-nicotinamide adenine dinucleotide, reductase, reduced nicotinamide adenine dinucleotide-ferredoxin, Rnf, XYLA, xylM

ECTree

     1 Oxidoreductases
         1.18 Acting on iron-sulfur proteins as donors
             1.18.1 With NAD+ or NADP+ as acceptor
                1.18.1.3 ferredoxin-NAD+ reductase

Purification

Purification on EC 1.18.1.3 - ferredoxin-NAD+ reductase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
Blue-sepharose column chromatography
-
electron-transfer component XylA
NADH-ferredoxin oxidoreductase component of biphenyl 2,3-dioxygenase
-
NADH-ferredoxinNAP reductase component of naphthalene dioxygenase
-
recombinant BphA4 under aerobic conditions from Escherichia coli JM109 cells by anion exchange and adsorption chromatography, and ultrafiltration
-
recombinant enzyme from Escherichia col strain BL21(DE3) by repeated anion exchange chromatography, using a linear gradient of NaCl in 20 mM HEPES buffer, pH 7.0, for elution, followed by gel filtration and ultrafiltration
-
recombinant His-tagged RedIIA from Escherichia coli strain BL21(DE3) by metal affinity chromatography and gel filtration
-
recombinant protein
-