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1.18.1.2: ferredoxin-NADP+ reductase

This is an abbreviated version!
For detailed information about ferredoxin-NADP+ reductase, go to the full flat file.

Word Map on EC 1.18.1.2

Reaction

2 reduced ferredoxin +

NADP+
+
H+
= 2 oxidized ferredoxin +
NADPH

Synonyms

ABO_0145, adrenodoxin reductase, AnFNR, BsFNR, CT1512, cytochrome b6f -associated ferredoxin:NADP+ oxidoreductase, DA1, EC 1.6.7.1, EC 1.6.99.4, Fd-NADP+ reductase, ferredoxin (flavodoxin)-(reduced) nicotinamide adenine dinucleotide phosphate reductase, ferredoxin (flavodoxin)-NAD(P)H reductase, ferredoxin (flavodoxin)-NADP(H) reductase, ferredoxin (flavodoxin):NADP+ oxidoreductase, ferredoxin NADP oxidoreductase, ferredoxin NADP reductase, ferredoxin NADP(H) oxidoreductase, ferredoxin NADP+ oxidoreductase, ferredoxin NADP+ reductase, ferredoxin nicotinamide adenine dinucleotide phosphate reductase, ferredoxin-NAD(P)+ oxidoreductase, ferredoxin-NAD(P)+ reductase, ferredoxin-NAD(P)H reductase, ferredoxin-NADP oxidoreductase, ferredoxin-NADP reductase, Ferredoxin-NADP(+) reductase, ferredoxin-NADP(H) oxidoreductase, ferredoxin-NADP(H) reductase, ferredoxin-NADP+ oxidoreductase, ferredoxin-NADP+ reductase, ferredoxin-NADP+-oxidoreductase, ferredoxin-NADP+-reductase, ferredoxin-NADP-oxidoreductase, ferredoxin-NADP-reductase, ferredoxin-nicotinamide adenine dinucleotide phosphate reductase, ferredoxin-nicotinamide-adenine dinucleotide phosphate (oxidized) reductase, ferredoxin-TPN reductase, ferredoxin: NADP(+) oxidoreductase, ferredoxin: NADP+ oxidoreductase, ferredoxin:NADP oxidoreductase, ferredoxin:NADP(+) oxidoreductase, ferredoxin:NADP(H) oxidoreductase, ferredoxin:NADP(H) oxidoreductase 2, ferredoxin:NADP+ oxidoreductase, ferredoxin:NADP+ reductase, ferredoxin:NADPH oxidoreductase, ferric reductase, Flavodoxin reductase, FLDR, FLXR, FNR, FNR-A, FNR-B, FNR1, FNR2, FNRII, FNRL, FNRS, FPR, FprA, FprB, Fprs, LFNR1, LFNR2, mitochondrial-type ferredoxin:NADP+ reductase, More, mtFNR, NADH-dependent reduced ferredoxin:NADP oxidoreductase, NADP:ferredoxin oxidoreductase, NADPH ferredoxin reductase, NADPH-dependent ferredoxin reductase, NADPH:ferredoxin oxidoreductase, NfnAB, NfnIII, NFR, PETH, PfFNR, pFNR, photosynthetic ferredoxin NADP+ oxidoreductase, photosynthetic FNR, plastidic-type ferredoxin-NADP+ reductase, PP4_41290, RcFPR, reduced nicotinamide adenine dinucleotide phosphate-adrenodoxin reductase, reductase, ferredoxin-nicotinamide adenine dinucleotide phosphate, RFNR2, ST2133, TgFNR, TPNH-ferredoxin reductase, YumC

ECTree

     1 Oxidoreductases
         1.18 Acting on iron-sulfur proteins as donors
             1.18.1 With NAD+ or NADP+ as acceptor
                1.18.1.2 ferredoxin-NADP+ reductase

Purification

Purification on EC 1.18.1.2 - ferredoxin-NADP+ reductase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
34 kDa enzyme form, 78 kDa complexed enzyme by cation exchange chromatography, and gel filtration, active 45 kDa enzyme form to homogeneity by ammonium sulfate precipitation, DEAE ion exchange chromatography, and gel filtration
ammonium sulfate precipitation and anion exchange column chromatography
ammonium sulfate precipitation, gel filtration, the TSK column chromatography, and MonoQ column chromatography
ammonium sulfate precipitation, Matrex Red A column chromatography, DEAE-5PW column chromatography, hydroxyapatite column chromatography, and Superdex-200 gel filtration
-
ammonium sulfate precipitation. gel filtration, the TSK column chromatography, and MonoQ column chromatography
butyl Toyopearl column chromatography and DEAE Sepharose column chromatography
DEAE-Sepharose column chromatography and Sephacryl S-300 gel filtration
-
expressed in Escherichia coli
-
from spinach leaves in several steps, to homogeneity
-
His-Trap column chromatography, Ni-NTA column chromatography, and S100 gel filtration
HisPur cobalt resin column chromatography and Superdex 200 gel filtration
HisTrap column chromatography
-
hydroxyapatite column chromatography and Mono Q column chromatography
-
native enzyme from cell extract 86fold to homogeneity by several chromatographic steps
-
Ni-NTA affinity chromatography
phenyl-Superose column chromatography
-
preparation of phycobilisomes by sucrose density gradient centrifugation
-
purification method development for cytochrome b6f -associated ferredoxin:NADP+ oxidoreductase, overview
Q-Sepharose Fast Flow column chromatography and Sephadex G-50 gel filtration
-
recombinant chimeric enzyme, comprising the root NADP-binding domain and the leaf FAD-binding domain, from Escherichia coli
-
recombinant enzyme from Escherichia coli to homogeneity, native enzyme about 400fold to homogeneity
recombinant His-tagged mtFNR to homogeneity from Escherichia coli by nickel affinity chromatography
-
recombinant NfnAB complex of Thermotoga maritima from Escherichia coli strain under aerobic conditions by ultracentrifugation, and heat treatment for 30 min at 80°C, the supernatant is further purified by affinity chromatography and ultrafiltration. Iron-sulfur cluster reconstitution in 100 mM Tris-HCl, pH 7.5, containing 8 mM DTT, 0.01 mM FAD, 2 mM cysteine, and 1.5 mM FeSO4 at room temperature for 1 h under strictly anaerobic conditions
-
recombinant wild-type and mutant enzymes from Escherichia coli
recombinant wild-type and mutant enzymes from Escherichia coli strain Bl21(DE3)
recombinant wild-type and mutant ferredoxin and Fd-NADP+ reductases from Escherichia coli strain BL21(DE3) the latter as homodimers, separation to monomers and crosslinking, further purification of crosslinked complexes by anion exchange chromatography and gel filtration
-
soluble enzyme by ultracentrifugation, DEAE ion exchange chromatography, dialysis, and hydroxyapatite chromatography
-
soluble recombinant enzyme from Escherichia coli strain BL21(DE3) to homogeneity by ammonium sulfate fractionation, gel filtration, and anion-exchange chromatography
-
TALON-cobalt column chromatography
-
ultrafiltration and Sephadex G-50 gel filtration
-