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8.9
1,4-dioxo-2-(trifluoromethyl)-1lambda5,4lambda5-quinoxaline
pH 7.4, 25°C
-
38
1,4-dioxo-7-(trifluoromethoxy)-1lambda5,2,4lambda5-benzotriazin-3-amine
pH 7.4, 25°C
-
4.3
1-oxo-1lambda5,2,4-benzotriazine
pH 7.4, 25°C
-
18
3-amino-1,4-dioxo-1lambda5,4lambda5-quinoxaline-2-carbonitrile
pH 7.4, 25°C
-
8000
Ferredoxin
Plasmodium falciparum ferredoxin PfFd is tested;using cytochrome c as electron acceptor
-
3700 - 33330
ferricytochrome c
48
methyl (1,4-dioxo-1lambda5,2,4lambda5-benzotriazin-3-yl)carbamate
pH 7.4, 25°C
-
26
N-(1,4-dioxo-1lambda5,2,4lambda5-benzotriazin-3-yl)-1,1,1-trifluoromethanesulfonamide
pH 7.4, 25°C
-
62
N-(1,4-dioxo-1lambda5,2,4lambda5-benzotriazin-3-yl)acetamide
pH 7.4, 25°C
-
7.9
N-(1,4-dioxo-1lambda5,2,4lambda5-benzotriazin-3-yl)methanesulfonamide
pH 7.4, 25°C
-
1.6
N-(1-oxo-1lambda5,2,4-benzotriazin-3-yl)acetamide
pH 7.4, 25°C
-
0.000000064 - 51370
NADPH
37700
oxidized Fdx2
pH 7.5, 25°C
-
500 - 39800
oxidized ferredoxin
12.5
reduced ferredoxin
pH 8.0, 25°C
76.3
Fe(III)-EDTA
in the presence of 0.15 mM Fe(III)-EDTA, in the absence of free flavin, in 50 mM sodium phosphate (pH 7.0), at 25°C
972
Fe(III)-EDTA
in the presence of 0.15 mM Fe(III)-EDTA, in the presence of 0.15 mM FAD, in 50 mM sodium phosphate (pH 7.0), at 25°C
3700
ferricytochrome c
-
mutant bearing a truncated N-terminal domain beginning after the KISKK domain, pH 7.5, temperature not specified in the publication
4520
ferricytochrome c
-
mutant bearing lysine to glutamine mutations in the second and third lysine residues of the KISKK domain, pH 7.5, temperature not specified in the publication
12640
ferricytochrome c
-
mutant with an extended N-terminal region to include the EAxxPA motif of maize, pH 7.5, temperature not specified in the publication
12830
ferricytochrome c
-
mutant bearing an N-terminal region with the full wheat KISKK domain, pH 7.5, temperature not specified in the publication
18940
ferricytochrome c
-
mutant bearing a lysine to glutamine mutation in the second lysine residue of the KISKK domain, pH 7.5, temperature not specified in the publication
31710
ferricytochrome c
-
mutant bearing a lysine to glutamine mutation in the first lysine residue of the KISKK domain, pH 7.5, temperature not specified in the publication
33330
ferricytochrome c
-
wild-type, pH 7.5, temperature not specified in the publication
200
flavodoxin
-
using cytochrome c as electron acceptor, for mutant W57E
-
350
flavodoxin
-
using cytochrome c as electron acceptor, for mutant I59E/I92E
-
450
flavodoxin
-
using cytochrome c as electron acceptor, for mutant I59A/I92A
-
690
flavodoxin
-
using cytochrome c as electron acceptor, for mutant W57R
-
700
flavodoxin
-
using cytochrome c as electron acceptor
-
1010
flavodoxin
-
using cytochrome c as electron acceptor, for mutant W57K
-
2380
flavodoxin
-
using cytochrome c as electron acceptor, for mutant I59A
-
2770
flavodoxin
-
using cytochrome c as electron acceptor, for mutant I92E
-
3090
flavodoxin
-
using cytochrome c as electron acceptor, for mutant I59E
-
3150
flavodoxin
-
using cytochrome c as electron acceptor, for mutant I92A
-
0.0004
NADH
mutant T155G/A160T/S223D/Y235F/L263P/R264P/G265P, called AMP2PP5
0.006
NADH
mutant T155G/A160T/S223D/L263P/R264P/G265P, called AMP1PP5
0.2
NADH
wild type enzyme of Anabaena sp.
0.5
NADH
wild-type, pH 8.0, 30°C
0.6
NADH
mutant lacking the beta-hairpin, pH 8.0, 30°C
0.8
NADH
mutant T155G/A160T/L263P, called PP3
0.8
NADH
mutant with additional tryptophan residue at the C-terminus, pH 8.0, 30°C
1
NADH
pH 8.0, temperature not specified in the publication
1.4
NADH
mutant T155G/A160T/L263P/R264P/G265P, called PP5
8
NADH
mutant DELTAW248, pH 8.0, 30°C
15
NADH
mutant with an insertion of the iloop of Pisum sativum enzyme plus deletion of residue W248, pH 8.0, 30°C
30
NADH
-
for FprA in a flavin free assay, using Fe(III)-citrate as electron acceptor
70
NADH
-
for FprA in a cytochrome c assay, using Pseudomonas putida [4Fe-4S]-ferredoxin FdxA as electron acceptor
90
NADH
-
for FprA in a cytochrome c assay, using Pseudomonas putida [2Fe-2S]-ferredoxin FdA as electron acceptor
90
NADH
-
for FprA in a flavin free assay, using 2,6-dichlorophenolindophenol as electron acceptor
110
NADH
-
for FprA in a flavin free assay, using Fe(III)-EDTA as electron acceptor
110
NADH
-
for FprA in a flavin free assay, using K3Fe(CN)6 as electron acceptor
130
NADH
-
for FprA in a cytochrome c assay, using Pseudomonas putida flavodoxin Fld as electron acceptor
170
NADH
-
for FprA in a cytochrome c assay, using Pseudomonas putida [2Fe-2S]-ferredoxin FdB as electron acceptor
540
NADH
-
for FprB in a cytochrome c assay, using Pseudomonas putida [2Fe-2S]-ferredoxin FdB as electron acceptor
550
NADH
-
for FprA with FAD as cofactor, using Fe(III)-EDTA as electron acceptor
680
NADH
-
for FprB in a flavin free assay, using 2,6-dichlorophenolindophenol as electron acceptor
800
NADH
mutant T155G/A160T/L263P/Y303S, called PP3CT
860
NADH
-
for FprB in a flavin free assay, using K3Fe(CN)6 as electron acceptor
930
NADH
-
for FprA with FAD as cofactor, using Fe(III)-citrate as electron acceptor
1060
NADH
-
for FprA with FMN as cofactor, using Fe(III)-EDTA as electron acceptor
1060
NADH
-
for FprB with FAD as cofactor, using Fe(III)-EDTA as electron acceptor
1170
NADH
-
for FprB in a flavin free assay, using Fe(III)-EDTA as electron acceptor
1860
NADH
-
for FprB in a cytochrome c assay, using Pseudomonas putida flavodoxin Fld as electron acceptor
2690
NADH
-
for FprB with FMN as cofactor, using Fe(III)-EDTA as electron acceptor
2870
NADH
-
for FprA with FMN as cofactor, using Fe(III)-citrate as electron acceptor
3130
NADH
-
for FprB with FAD as cofactor, using Fe(III)-citrate as electron acceptor
3270
NADH
-
for FprB in a flavin free assay, using Fe(III)-citrate as electron acceptor
4630
NADH
-
for FprB in a cytochrome c assay, using Pseudomonas putida [4Fe-4S]-ferredoxin FdxA as electron acceptor
6730
NADH
-
for FprB in a cytochrome c assay, using Pseudomonas putida [2Fe-2S]-ferredoxin FdA as electron acceptor
7090
NADH
-
for FprB with FMN as cofactor, using Fe(III)-citrate as electron acceptor
0.000000064
NADPH
-
pH 7.4, 25°C, recombinant enzyme
0.000000064
NADPH
-
pH 8.2, 25°C, recombinant enzyme
0.0000009
NADPH
-
pH 6.8, 25°C, recombinant enzyme
0.0002
NADPH
mutant T155G/A160T/S223D/L263P/R264P/G265P, called AMP1PP5
20
NADPH
in an assay using K3Fe(CN)6 as electron acceptor for mutant H286L
45
NADPH
in an assay using 2,6-dichlorophenolindophenol as electron acceptor for mutant H286K
49
NADPH
in an assay using 2,6-dichlorophenolindophenol as electron acceptor for mutant H286A
85
NADPH
in an assay using K3Fe(CN)6 as electron acceptor for mutant H286A
90
NADPH
-
for FprB in a flavin free assay, using Fe(III)-EDTA as electron acceptor
530
NADPH
-
for FprA in a cytochrome c assay, using Pseudomonas putida [2Fe-2S]-ferredoxin FdB as electron acceptor
720
NADPH
-
for FprB with FAD as cofactor, using Fe(III)-EDTA as electron acceptor
750
NADPH
in an assay using 2,6-dichlorophenolindophenol as electron acceptor
770
NADPH
-
for FprB in a cytochrome c assay, using Pseudomonas putida [2Fe-2S]-ferredoxin FdB as electron acceptor
770
NADPH
-
for FprB with FMN as cofactor, using Fe(III)-EDTA as electron acceptor
840
NADPH
-
for FprA with FAD as cofactor, using Fe(III)-EDTA as electron acceptor
930
NADPH
-
for FprB with FAD as cofactor, using Fe(III)-citrate as electron acceptor
1000
NADPH
-
for FprA in a flavin free assay, using 2,6-dichlorophenolindophenol as electron acceptor
1130
NADPH
-
for FprB in a flavin free assay, using K3Fe(CN)6 as electron acceptor
1140
NADPH
-
for FprB in a flavin free assay, using 2,6-dichlorophenolindophenol as electron acceptor
1190
NADPH
-
for FprA with FAD as cofactor, using Fe(III)-citrate as electron acceptor
1200
NADPH
using cytochrome c as electron acceptor
1300
NADPH
using 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium chloride as electron acceptor
1320
NADPH
-
for FprA in a flavin free assay, using Fe(III)-EDTA as electron acceptor
1370
NADPH
-
for FprA in a cytochrome c assay, using Pseudomonas putida [2Fe-2S]-ferredoxin FdA as electron acceptor
1500
NADPH
mutant T155G/A160T/L263P/R264P/G265P, called PP5
1570
NADPH
-
for FprA in a flavin free assay, using Fe(III)-citrate as electron acceptor
1680
NADPH
-
for FprA with FMN as cofactor, using Fe(III)-EDTA as electron acceptor
1700
NADPH
mutant with additional tryptophan residue at the C-terminus, pH 8.0, 30°C
2000
NADPH
with 2,6-dichlorophenolindophenol as cosubstrate, at pH 7.0 and 25°C
2090
NADPH
-
for FprB in a flavin free assay, using Fe(III)-citrate as electron acceptor
3050
NADPH
-
for FprA in a cytochrome c assay, using Pseudomonas putida [4Fe-4S]-ferredoxin FdxA as electron acceptor
3150
NADPH
in an assay using 2,6-dichlorophenolindophenol as electron acceptor for mutant H286Q
3230
NADPH
-
for FprA in a flavin free assay, using K3Fe(CN)6 as electron acceptor
3300
NADPH
in an assay using K3Fe(CN)6 as electron acceptor for mutant H286Q
3400
NADPH
-
enzyme in soluble extract, with oxidized 2,6-dichlorophenolindophenol as cosubstrate, at pH 8.0, temperature not specified in the publication
3500
NADPH
in an assay using K3Fe(CN)6 as electron acceptor
3720
NADPH
-
for FprB in a cytochrome c assay, using Pseudomonas putida flavodoxin Fld as electron acceptor
4590
NADPH
-
for FprB in a cytochrome c assay, using Pseudomonas putida [4Fe-4S]-ferredoxin FdxA as electron acceptor
4600
NADPH
wild-type, pH 8.0, 30°C
6040
NADPH
-
for FprA with FMN as cofactor, using Fe(III)-citrate as electron acceptor
6400
NADPH
mutant T155G/A160T/L263P, called PP3
6800
NADPH
mutant with an insertion of the iloop of Pisum sativum enzyme, pH 8.0, 30°C
6900
NADPH
-
enzyme in PBS, with oxidized 2,6-dichlorophenolindophenol as cosubstrate, at pH 8.0, temperature not specified in the publication
8500
NADPH
using K3Fe(CN)6 as electron acceptor
8526
NADPH
pH 7.0, 25°C, recombinant mutant Y50S
9400
NADPH
mutant T155G/A160T/L263P/Y303S, called PP3CT
9953
NADPH
pH 7.0, 25°C, recombinant mutant Y50G
11000
NADPH
-
for FprB in a cytochrome c assay, using Pseudomonas putida [2Fe-2S]-ferredoxin FdA as electron acceptor
11300
NADPH
pH 8.0, temperature not specified in the publication
11510
NADPH
-
for FprA in a cytochrome c assay, using Pseudomonas putida flavodoxin Fld as electron acceptor
13500
NADPH
wild type enzyme of Anabaena sp.
15000
NADPH
-
with 2 mM ferricyanide in 20 mM HEPES-NaOH buffer, at pH 7.0 and 25°C
17000
NADPH
-
for FprB with FMN as cofactor, using Fe(III)-citrate as electron acceptor
17100
NADPH
mutant lacking the beta-hairpin, pH 8.0, 30°C
24500
NADPH
wild-type, pH 8.0, 30°C
33000
NADPH
mutant with an insertion of the iloop of Pisum sativum enzyme plus deletion of residue W248, pH 8.0, 30°C
39800
NADPH
mutant DELTAW248, pH 8.0, 30°C
45750
NADPH
pH 7.0, 25°C, recombinant mutant Y50W
51370
NADPH
pH 7.0, 25°C, recombinant wild-type enzyme
500
oxidized ferredoxin
mutant with an insertion of the iloop of Pisum sativum enzyme plus deletion of residue W248, pH 8.0, 30°C
740
oxidized ferredoxin
mutant DELTAW248, pH 8.0, 30°C
6440
oxidized ferredoxin
pH 7.5, 25°C
10700
oxidized ferredoxin
mutant with an insertion of the iloop of Pisum sativum enzyme, pH 8.0, 30°C
16300
oxidized ferredoxin
wild-type, pH 8.0, 30°C
34100
oxidized ferredoxin
wild-type, pH 8.0, 30°C
36300
oxidized ferredoxin
mutant lacking the beta-hairpin, pH 8.0, 30°C
39800
oxidized ferredoxin
mutant with additional tryptophan residue at the C-terminus, pH 8.0, 30°C