Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

1.18.1.1: rubredoxin-NAD+ reductase

This is an abbreviated version!
For detailed information about rubredoxin-NAD+ reductase, go to the full flat file.

Word Map on EC 1.18.1.1

Reaction

reduced rubredoxin
+
NAD+
 +
H+
=
oxidized rubredoxin
 +
NADH

Synonyms

(flavo)rubredoxin reductase, ABO_0162, dihydronicotinamide adenine dinucleotide-rubredoxin reductase, DPNH-rubredoxin reductase, EC 1.6.7.2, FIRd-reductase, NAD(P)H:rubredoxin reductase, NADH-rubredoxin oxidoreductase, NADH-rubredoxin reductase, NADH: rubredoxin oxidoreductase, NADH:rubredoxin oxidoreductase, NOR, NROR, RdxR, reduced nicotinamide adenine dinucleotide-rubredoxin reductase, reductase, rubredoxin-nicotinamide adenine dinucleotide, rubB, rubredoxin reductase, rubredoxin-NAD reductase

ECTree

     1 Oxidoreductases
         1.18 Acting on iron-sulfur proteins as donors
             1.18.1 With NAD+ or NADP+ as acceptor
                1.18.1.1 rubredoxin-NAD+ reductase

Crystallization

Crystallization on EC 1.18.1.1 - rubredoxin-NAD+ reductase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
purified recombinant His6-tagged enzyme, sitting-drop vapour-diffusion method, 20°C, 0.001 l of 14 mg/ml protein in 20 mM Tris-HCl, pH 7.0, is mixed with 0.001 ml of reservoir solution containing 35% v/v PEG 400, 0.1 M Tris-HCl, pH 8.5, and 0.15 M MgCl2, and equilibrated against 0.1 ml of reservoir solution, X-ray diffraction structure determination and analysis at 2.1 A resolution
purified recombinant His6-tagged enzyme, sitting-drop vapour-diffusion method, 20°C, 0.001 l of 14 mg/ml protein in 20 mM Tris-HCl, pH 7.0, is mixed with 0.001 ml of reservoir solution containing 35% v/v PEG 400, 0.1 M Tris-HCl, pH 8.5, and 0.15 M MgCl2, and equilibrated against 0.1 ml of reservoir solution, X-ray diffraction structure determination and analysis at 2.1 A resolution, structure modelling, overview
purified recombinant His-tagged enzyme, free or in complex with substrate rubredoxin, sitting drop vapour diffusion method, 20°C, 8.5 mg/ml protein in 100 mM NaCl, 50 mM Tris-HCl, pH 8.0, FAD, and 5 mM 2-mercaptoethanol, in presence or absence of rubredoxin in a 1.2 molar excess, mixing with an equal volume of reservoir solution containing 5% PEG 1000, 40% PEG 300, 0.1 M Tris-HCl, pH 7.0, mother liquor supplemented with 25% PEG 400 is used for cryoprotection, X-ray diffraction structure determination and analysis at 2.3-2.4 A resolution