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1.17.7.4: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate reductase

This is an abbreviated version!
For detailed information about 4-hydroxy-3-methylbut-2-en-1-yl diphosphate reductase, go to the full flat file.

Word Map on EC 1.17.7.4

Reaction

dimethylallyl diphosphate
+ 2 oxidized ferredoxin [iron-sulfur] cluster +
H2O
=
(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate
 + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+

Synonyms

(E)-4-hydroxy-3-methylbut-2-enyl diphosphate reductase, 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate reductase, 1-hydroxy-2-methyl-2-(E)-butenyl-4-diphosphate reductase, 4-hydroxy-3-methylbut-2-enyl diphosphate reductase, 4-hydroxy-3-methylbut-2-enyl diphosphate reductase 1, 4-hydroxy-3-methylbut-2-enyl pyrophosphate reductase, BgHDR1, BgHDR2, chloroplast biogenesis6, Clb6, DsHDR, E-4-hydroxy-3-methyl-but-2-enyl diphosphate reductase, EC 1.17.1.2, HDR, HDR1, HMBPP reductase, HsHDR1, HsHDR2, isoprenoid synthesis H, ispH, lysis-tolerant B, LytB, reductase, (E)-4-hydroxy-3-methylbut-2-enyl diphosphate, SrHDR

ECTree

     1 Oxidoreductases
         1.17 Acting on CH or CH2 groups
             1.17.7 With an iron-sulfur protein as acceptor
                1.17.7.4 4-hydroxy-3-methylbut-2-en-1-yl diphosphate reductase

Crystallization

Crystallization on EC 1.17.7.4 - 4-hydroxy-3-methylbut-2-en-1-yl diphosphate reductase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
purified recombinant IspH, sitting drop vapour diffusion method at 20°C, 0.004 ml of 35 /ml protein in 50 mM Tris-HCl, pH 8.0, is mixed with 0.004 ml of reservoir solution containing 100 mM Bis-Tris-HCl, pH 6.5, 200 mM lithium sulfate and 25% PEG 3350, final pH is 6.8, crystal soaking in 2 M lithium sulfate, pH 6.5, before freezing, X-ray diffraction structure determination and analysis at 1.4 A resolution, molecular replacement, modelling
-
crystallized under anaerobic conditions. Its structure is determined by the multiple anomalous dispersion method and refined to final R/Rfree factors of 17.5/19.5% at 2.2 A resolution. LytB is a momoner which is essentially composed of three related alpha/beta-domains arranged in a cloverleaf form with the Fe/S-cluster in its center
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