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1.17.4.2: ribonucleoside-triphosphate reductase (thioredoxin)

This is an abbreviated version!
For detailed information about ribonucleoside-triphosphate reductase (thioredoxin), go to the full flat file.

Word Map on EC 1.17.4.2

Reaction

2'-deoxyribonucleoside 5'-triphosphate
+
thioredoxin disulfide
 +
H2O
=
ribonucleoside 5'-triphosphate
 +
thioredoxin

Synonyms

2'-deoxyribonucleoside-triphosphate:oxidized-thioredoxin 2'-oxidoreductase, adenosylcobalamin-dependent ribonucleoside-triphosphate reductase, class Ib ribonucleotide reductase, class Ib RNR, class II ribonucleotide reductase, class II RNR, class III ribonucleotide reductase, class III RNR, More, nrdD, nucleoside triphosphate reductase, ribonucleoside triphosphate reductase, ribonucleotide diphosphate reductase, ribonucleotide reductase, RNR, RNR class Ia, RNR2, RTPR

ECTree

     1 Oxidoreductases
         1.17 Acting on CH or CH2 groups
             1.17.4 With a disulfide as acceptor
                1.17.4.2 ribonucleoside-triphosphate reductase (thioredoxin)

Cloned

Cloned on EC 1.17.4.2 - ribonucleoside-triphosphate reductase (thioredoxin)

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
chromosomal DNA containg RNR gene PCR amplified, gene cloned, completely sequenced and expressed in Escherichia coli
-
cloned by PCR from peptide sequence information
-
cloned by PCR from peptide sequence information, nrdj gene sequenced completely and expressed in Escherichia coli BL21(DE3)
-
cloning, sequencing and expression of the protein
-
gene encoding p53R2 genotyping, real-time RT-PCR expression analysis
-
high-level overexpression of RNR subunits, Rrm1, Rrm2 and p53R2, separately or in different combinations, in mice, simultaneous expression of two RNR subunits
-
overexpressed in Escherichia coli
-
subunit R2
-
the nrdDG promoter regulates transcriptional expression of the anaerobic ribonucleotide reductase of Escherichia coli, binding of the pleiotropic FNR, fumarate and nitrate reduction, transcriptional regulator to the nrdDG promoter region affects the transcription, the upstream FNR-2 site is essential for anaerobic activation of the nrdDG promoter. Although the FNR-1 site is not absolutely required, it allows maximal expression of this promoter, overview
-
two-hybrid interaction analysis of CSN7 and RNR2 expressing the vectors under control of the 35S promoter in Nicotiana benthamiana cells, transgenic expression of RNR2 in Arabidopsis thaliana plants