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1.17.4.1: ribonucleoside-diphosphate reductase

This is an abbreviated version!
For detailed information about ribonucleoside-diphosphate reductase, go to the full flat file.

Word Map on EC 1.17.4.1

Reaction

2'-deoxyribonucleoside 5'-diphosphate
+
thioredoxin disulfide
 +
H2O
=
ribonucleoside 5'-diphosphate
 +
thioredoxin

Synonyms

2'-deoxyribonucleoside-diphosphate:oxidized-thioredoxin 2'-oxidoreductase, ADP reductase, CDP reductase, class I ribonucleotide reductase, class I ribonulceotide reductase, class I RNR, class I RR, class Ia ribonucleotide reductase, class Ia RNR, class Ia RR, class Ib ribonucleotide reductase, class Ib RNR, class Ic ribonucleotide reductase, class II RNR, manganese-ribonucleotide reductase, Mn-RNR, More, mRR, NrdA, NrdB, NrdE, NrdF, nucleoside diphosphate reductase, p53-inducible ribonucleotide reductase, R2F, reductase, ribonucleoside diphosphate, ribonucleoside 5'-diphosphate reductase, ribonucleoside diphosphate reductase, ribonucleoside-diphosphate reductase subunit M2 B, ribonucleotide diphosphate reductase, ribonucleotide reductase, RIR1, RIR2, RNR, RNR1 rRibonucleoside-diphosphate reductase large chain 1, RNR2, UDP reductase

ECTree

     1 Oxidoreductases
         1.17 Acting on CH or CH2 groups
             1.17.4 With a disulfide as acceptor
                1.17.4.1 ribonucleoside-diphosphate reductase

Cloned

Cloned on EC 1.17.4.1 - ribonucleoside-diphosphate reductase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21 (DE3)
expression analysis of AtRNR2-like catalytic subunit gene AtRNR2A, phylogenetic analysis. Transcriptional changes of 17-days-old Arabidopsis plants, enriched in S-phase cells over younger seedlings, in response to the replication-blocking agent hydroxyurea and to the DNA double-strand break inducer bleomycin
expression analysis of AtRNR2-like catalytic subunit gene AtRNR2B, phylogenetic analysis. Transcriptional changes of 17-days-old Arabidopsis plants, enriched in S-phase cells over younger seedlings, in response to the replication-blocking agent hydroxyurea and to the DNA double-strand break inducer bleomycin
expression analysis of mouse RRM2 protein in Hep-G2 cells
-
expression analysis of the AtRNR2-like catalytic subunit gene TSO2, phylogenetic analysis. Transcriptional changes of 17-days-old Arabidopsis plants, enriched in S-phase cells over younger seedlings, in response to the replication-blocking agent hydroxyurea and to the DNA double-strand break inducer bleomycin
expression in Escherichia coli
expression in Escherichia coli BL-21 (DE3)
expression in Escherichia coli BL21 (DE3)
expression in Escherichia coli BL21(DE3) cells
expression of chimeric R2 genes in which C-terminal sequences in Escherichia coli subunit R2 are replaced by C-terminal sequences from the mouse subunit R2
-
expression of His-tagged alpha and beta subunits and of His-tagged mutant alpha-subunit
-
expression of His-tagged subunits R2 and R4 in Escherichia coli strain BL21(DE3)
expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3)
-
expression of His6-tagged subunits hRRM1 and hRRM2 in Escherichia coli strain BL21(DE3)
-
expression of His6-tagged subunits M2, p53R2, and M1 in Escherichia coli strain BL21(DE3)
-
expression of M1 mutant cDNA containing a G to A transition at codon 57 in chinese hamster ovary cells
expression of N-terminally tagged NrdE and NrdF, the RNR genes are organized within the nrdI-nrdE-nrdF-ymaB operon, recombinant expression of NrdI, NdE, NrdF, and YmaB in Escherichia coli. For overexpression, the entire operon is placed behind a Pspank(hy) promoter and integrated into the Bacillus subtilis genome at the amyE site. Removal of the tag from rNrdE and rNrdF, leaving three amino acids GSH, has little effect on activity
-
expression of nrdA and nrdB genes encoding a CDP reductase in Escherichia coli
-
expression of R2F in Escherichia coli strain BL21(DE3)
-
expression of subunits Y1, Y2, Y3 and Y4 in Escherichia coli, expression of Y1, His-tagged Y2 and His tagged Y2-K387N mutant enzyme subunit in Saccharomyces cerevisiae
-
expression of wild-type and amino terminal deleted DELTA1-248 R1 subunit and wild-type, F127Y, Y129F and F127Y/Y129F mutant R2 subunit in Escherichia coli
-
expression of wild-type and Y177F mutant subunit R2 in Escherichia coli
-
expression of wild-type R2 and R1 and of truncated R1 mutant DELTA1-248 in Escherichia coli
-
expression of Y122F, Y356F and Y122F/Y356F mutant enzymes in Escherichia coli
expression of Y370F and Y370W mutant enzymes in Escherichia coli
-
expression R1E and R2F subunits in Escherichia coli
gene Rv3051c, expression of the His-tagged enzyme in Escherichia coli strain Bl21(DE3)
-
genes nrdA and nrdB, expression of FLAG-tagged subunits R1 and R2 in Escherichia coli strains CMT931 and CMT933
-
genes V3 and St1, gene mapping, DNA and amino acid sequence determination and analysis
genes V3 and St1, gene mapping, DNA and amino acid sequence determination and analysis. Complementation of a yeast mutant
Herpes simplex virus type I and II
Herpes simplex virus
-
nearly full length cDNA of M1 and M2 subunits, expression of M2 subunit in mouse 3T6 cells and monkey COS-7 cells
-
overexpression of B2 subunit
-
overexpression of wild-type and mutant R2 subunits in Escherichia coli strain BL21(DE3)
-
recombinant expression of R2F using expression vector pOCA2
-
recombinant overexpression of the ORF60 protein, subunit R2, with an N-terminal extension, MGPHHHHHHLESTSLYKKAGS in Escherichia coli BL21 (DE3) cells. The N-terminal extension encompasses an attB1 site for the gateway recombination event and a His tag to facilitate protein purification
-