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1.17.3.2: xanthine oxidase

This is an abbreviated version!
For detailed information about xanthine oxidase, go to the full flat file.

Word Map on EC 1.17.3.2

Reaction

xanthine
+
H2O
 +
O2
=
Urate
 +
H2O2

Synonyms

AXOR, EC 1.1.3.22, EC 1.2.3.2, EC 1.2.3.2., hypoxanthine oxidase, hypoxanthine-xanthine oxidase, hypoxanthine:oxygen oxidoreductase, More, oxidase, xanthine, Schardinger enzyme, xanthine dehydrogenase/oxidase, xanthine oxidase, xanthine oxidoreductase, xanthine: oxygen oxidoreductase, xanthine:O2 oxidoreductase, xanthine:oxygen oxidoreductase, xanthine:xanthine oxidase, XnOx, XO, XOD, XOR

ECTree

     1 Oxidoreductases
         1.17 Acting on CH or CH2 groups
             1.17.3 With oxygen as acceptor
                1.17.3.2 xanthine oxidase

Purification

Purification on EC 1.17.3.2 - xanthine oxidase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
60% ammonium sulfate, benzamidine-Sepharose
-
ammonium sulfate precipitation and affinity chromatography
-
ammonium sulfate, DEAE-Sepaharose
-
ammonium sulfate, hydroxyapatite, DEAE-cellulose
-
from liver
-
native enzyme 1645fold from milk to homogeneity by ammonium sulfate fracionation and affinity chromatography using a resin with diazotizated p-aminobenzamidine coupled to L-tyrosine, elution by benzamidine
native enzyme from fresh bovine cream
-
native enzyme from milk by ammonium sulfate fractionation, followed by affinity chromatography on heparin-agarose
native enzyme partially by gel filtration from fresh unpasteurized milk
purification of the molybdenum cofactor of milk xanthine oxidase
-
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli by nickel affinity and anion exchange chromatography, followed by gel filtration
recombinant His-tagged XDH1 from Pichia pastoris by nickel affinity and anion exchange chromatography
-
recombinant wild-type and mutant enzymes from Escherichia coli strain JM109 by anion exchange and hydroxyapatite chromatography, ultrafiltration, and gel filtration
-
simultaneous purification of xanthine oxidase and aldehyde oxidase
-
using a negative immune protocol with a higher activity recovery at 92.0%
-
using ammonium sulfate precipitation, affinity chromatography, and DEAE ion-exchange chromatography for refining enzyme
-