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1.17.1.4: xanthine dehydrogenase

This is an abbreviated version!
For detailed information about xanthine dehydrogenase, go to the full flat file.

Word Map on EC 1.17.1.4

Reaction

xanthine
+
NAD+
 +
H2O
=
Urate
 +
NADH
 +
H+

Synonyms

AtXDH1, EC 1.1.1.204, EC 1.2.1.37, IAO1, More, NAD-xanthine dehydrogenase, PaoABC, Retinol dehydrogenase, Rosy locus protein, VvXDH, xanthine dehydrogenase, xanthine dehydrogenase-1, xanthine dehydrogenase-2, xanthine dehydrogenase/oxidase, xanthine oxidoreductase, xanthine-NAD oxidoreductase, xanthine/NAD+ oxidoreductase, xanthine:NAD+ oxidoreductase, XDH, XDH/XO, XDH1, XDH2, XdhC, XOR, YagR, YagS, YagT

ECTree

     1 Oxidoreductases
         1.17 Acting on CH or CH2 groups
             1.17.1 With NAD+ or NADP+ as acceptor
                1.17.1.4 xanthine dehydrogenase

Purification

Purification on EC 1.17.1.4 - xanthine dehydrogenase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
529fold purification, several (NH4)2SO4 precipitation steps
-
adenine may play a role in preventing the dehydrogenase to oxidase conversion during extract preparation, storage, overnight dialysis and heat treatment
-
after expression in Escherichia coli
-
ammonium sulfate precipitation, phenyl-Sepharose column chromatography, DEAE-10 Macro-Prep MP10 column chromatography, and Bio-Prep SE-1000/17 column chromatography
-
during purification conversion from dehydrogenase type D to oxidase type O, reversible by dithioerythritol
-
from liver
-
highly active bovine XOR in its XDH form is prepared by folate affinity chromatography
-
immobilized on n-octylamine-substituted Sepharose 4B
-
in presence of dithiothreitol
-
native enzyme
-
native enzyme partially from mammary gland epithelial cells by gel filtration
-
near homogeneity, chromatography techniques
nearly homogenous, dehydrogenase type D, chromatography steps, oxidase type O purified by nearly the same procedure, but heated to 60°C after homogenization
-
partial
purification of native enzyme
-
purification of native XDH
purified as antibody complex
-
recombinant His-tagged enzyme from Escherichia coli strain TP1000 by nickel affinity and anion exchange chromatography, followed by gel filtration
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli by nickel affinity and anion exchange chromatography, followed by gel filtration
recombinant His-tagged wild-type and mutant XDH1 variants from Pichia pastoris strain KM71 by nickel affinity chromatography and anion exchange chromatography
recombinant His-tagged wild-type and mutant XDHs from Escherichia coli strain TP1000 by nickel affinity and anion exchange chromatography, followed by ultrafiltration and gel filtration
-
recombinant His-tagged XDH1 from Pichia pastoris by nickel affinity and anion exchange chromatography
recombinant wild-type and mutant enzymes with or without molybdenum cofactor by nickel affinity and anion exchange chromatography, followed by gel filtration
to crystalline state, chromatography techniques
-
to homogeneity, 2 types: dehydrogenase type, chromatography techniques, oxidase type: affinity chromatography
-
to homogeneity, affinity chromatography
to homogeneity, chromatography steps
-
to homogeneity, chromatography techniques
to homogeneity, chromatography techniques, preparative gel electrophoresis
-
to homogeneity, chromatography, preparative gel electrophoresis
-
to homogeneity, immobilized preparation
-
to homogeneity, immunoabsorption chromatography
-
to homogeneity, immunoaffinity chromatography
-
to homogeneity, mainly ferricyanide- and NAD+-linked activities, chromatography steps
-
to homogeneity, mainly NAD+-dependent activity
-
to homogeneity, precipitation and chromatography techniques
-
to homogeneity, presence of DTT required for purification of the dehydrogenase form
-
to homogeneity, recombinant enzyme
-