1.14.99.53: lytic chitin monooxygenase
This is an abbreviated version!
For detailed information about lytic chitin monooxygenase, go to the full flat file.
Word Map on EC 1.14.99.53
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1.14.99.53
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polysaccharide
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chitinases
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lpmos
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recalcitrant
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chitinolytic
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cellulose
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biomass
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c1-oxidizer
-
analysis
-
synthesis
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listeria
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transposon
-
monocytogenes
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degradation
- 1.14.99.53
- polysaccharide
- chitinases
-
lpmos
-
recalcitrant
-
chitinolytic
- cellulose
- biomass
-
c1-oxidizer
- analysis
- synthesis
-
listeria
- transposon
- monocytogenes
- degradation
Reaction
Synonyms
AA10, AA10A, AA9A, AO090102000501, BATR1942_08650, BURPS1710b_0114, CBM33A, CBP21, Cbp33A, CelS2, chitin-binding domain 3 protein, EF_0362, G15G9.090, GbpA, Jden_1381, lmo2467, LPMO10, LPMO10A, LPMO10B, LPMO10F, Micau_1630, PMO-2, RBAM17540, SCO0643, SGR_6855
ECTree
1.14.99.53 lytic chitin monooxygenaseAdvanced search results
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results (Substrates Products
Substrates Products on EC 1.14.99.53 - lytic chitin monooxygenase
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
alpha-chitin + ascorbate + O2
chito-oligosaccharide aldonic acid + dehydroascorbate + H2O
-
products show a degree of polymerization from DP3 to DP8, with DP6 being the most abundant product after 24 h of incubation
-
?
alpha-chitin + ascorbate + O2
oxidized chitooligosaccharides + dehydroascorbate + H2O
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-
-
?
beta-chitin + acceptor + O2
chito-oligosaccharide aldonic acid + reduced acceptor + H2O
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products show a degree of polymerization from DP3 to DP8, with DP6 being the most abundant product after 24 h of incubation
-
?
beta-chitin + ascorbic acid + O2
? + dehydroascorbate + H2O
-
-
-
-
?
beta-chitin + cellobiose dehydrogenase + O2
C1-oxidized oligosaccharides + reduced cellobiose dehydrogenase + H2O
chitin + acceptor + O2
[chitin oligosaccharide]-N-acetyl-D-glucosaminate + [chitin oligosaccharide]-N-acetyl-D-glucosamino-1,5-lactone
colloidal chitin + ascorbate + O2
oxidized chitin oligosaccharides + dehydroascorbate + H2O
colloidal chitin + ascorbate + O2
[chitin oligosaccharide]-N-acetyl-D-glucosaminate + [chitin oligosaccharide]-N-acetyl-D-glucosamino-1,5-lactone + dehydroascorbate + H2O
phosphoric acid swollen cellulose + ascorbate + O2
C4-oxidized cellooligosaccharides + C1/C4-oxidized cellooligosaccharides + dehydroascorbate + H2O
reaction of EC 1.14.99.54
-
-
?
[(1->4)-N-acetyl-beta-D-glucosaminyl]6 + ascorbate + O2
[(1->4)-N-acetyl-beta-D-glucosaminyl]3-(1->4)-N-acetyl-2-deoxy-2-amino-D-glucono-1,5-lactone + [(1->4)-N-acetyl-beta-D-glucosaminyl]2 + dehydroascorbate + H2O
-
-
-
?
[(1->4)-N-acetyl-beta-D-glucosaminyl]n+m + reduced acceptor + O2
[(1->4)-N-acetyl-beta-D-glucosaminyl]m-1-(1->4)-N-acetyl-2-deoxy-2-amino-D-glucono-1,5-lactone + [(1->4)-N-acetyl-beta-D-glucosaminyl]n + acceptor + H2O
alpha-chitin + acceptor + O2
oligosaccharide aldonic acids + reduced acceptor + H2O
-
-
-
-
?
alpha-chitin + acceptor + O2
oligosaccharide aldonic acids + reduced acceptor + H2O
-
-
-
-
?
alpha-chitin + ascorbate + O2
? + dehydroascorbate + H2O
-
products are the aldonic acid forms of fully acetylated chitooligosaccharides, oxidation occurs at the C1 carbon atom
-
?
alpha-chitin + ascorbate + O2
? + dehydroascorbate + H2O
-
products are the aldonic acid forms of fully acetylated chitooligosaccharides, oxidation occurs at the C1 carbon atom
-
?
alpha-chitin + ascorbate + O2
C1-oxidized chitooligosaccharides + dehydroascorbate + H2O
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products are C1-oxidized chitooligomers, mainly tetramers and hexamers
-
?
alpha-chitin + ascorbate + O2
C1-oxidized chitooligosaccharides + dehydroascorbate + H2O
-
products are C1-oxidized chitooligomers, mainly tetramers and hexamers
-
?
alpha-chitin + ascorbate + O2
C1-oxidized chitooligosaccharides + dehydroascorbate + H2O
enzyme shows stronger binding and a greater release of soluble oxidized products with beta-chitin than with alpha-chitin
products show oxidation of the C1 carbon leading to aldonic acids
-
?
alpha-chitin + ascorbate + O2
C1-oxidized chitooligosaccharides + dehydroascorbate + H2O
enzyme shows stronger binding and a greater release of soluble oxidized products with beta-chitin than with alpha-chitin
products show oxidation of the C1 carbon leading to aldonic acids
-
?
alpha-chitin + ascorbate + O2
oxidized oligosaccharides + dehydroascorbate + H2O
-
-
-
?
alpha-chitin + ascorbate + O2
oxidized oligosaccharides + dehydroascorbate + H2O
-
-
-
?
beta-chitin + acceptor + O2
oligosaccharide aldonic acids + reduced acceptor + H2O
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beta chitin from squid pen, best substrate
strong preference towards even-numbered products
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?
beta-chitin + acceptor + O2
oligosaccharide aldonic acids + reduced acceptor + H2O
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beta chitin from squid pen, best substrate
strong preference towards even-numbered products
-
?
beta-chitin + ascorbate + O2
? + dehydroascorbate + H2O
-
products are the aldonic acid forms of fully acetylated chitooligosaccharides, oxidation occurs at the C1 carbon atom
-
?
beta-chitin + ascorbate + O2
? + dehydroascorbate + H2O
-
products are the aldonic acid forms of fully acetylated chitooligosaccharides, oxidation occurs at the C1 carbon atom
-
?
beta-chitin + ascorbate + O2
C1-oxidized chitooligosaccharides + dehydroascorbate + H2O
beta-chitin is preferred over alpha-chitin
products are C1-oxidized chitooligomers, mainly tetramers and hexamers
-
?
beta-chitin + ascorbate + O2
C1-oxidized chitooligosaccharides + dehydroascorbate + H2O
beta-chitin is preferred over alpha-chitin
products are C1-oxidized chitooligomers, mainly tetramers and hexamers
-
?
beta-chitin + ascorbate + O2
C1-oxidized chitooligosaccharides + dehydroascorbate + H2O
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-
-
?
beta-chitin + ascorbate + O2
C1-oxidized chitooligosaccharides + dehydroascorbate + H2O
enzyme shows stronger binding and a greater release of soluble oxidized products with beta-chitin than with alpha-chitin
products show oxidation of the C1 carbon leading mainly to tetrameric and hexameric aldonic acids
-
?
beta-chitin + ascorbate + O2
C1-oxidized chitooligosaccharides + dehydroascorbate + H2O
enzyme shows stronger binding and a greater release of soluble oxidized products with beta-chitin than with alpha-chitin
products show oxidation of the C1 carbon leading mainly to tetrameric and hexameric aldonic acids
-
?
beta-chitin + ascorbate + O2
C1-oxidized chitooligosaccharides + dehydroascorbate + H2O
-
-
-
-
?
beta-chitin + ascorbate + O2
C1-oxidized oligosaccharides + dehydroascorbate + H2O
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-
-
?
beta-chitin + ascorbate + O2
C1-oxidized oligosaccharides + dehydroascorbate + H2O
substrate squid pen beta-chitin
in addition, considerable amounts of partially deacetylated oligomers are produced
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?
beta-chitin + ascorbate + O2
C1-oxidized oligosaccharides + dehydroascorbate + H2O
substrate squid pen beta-chitin
in addition, considerable amounts of partially deacetylated oligomers are produced
-
?
beta-chitin + ascorbate + O2
C1-oxidized oligosaccharides + dehydroascorbate + H2O
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-
-
?
beta-chitin + ascorbate + O2
oxidized oligosaccharides + dehydroascorbate + H2O
-
-
-
?
beta-chitin + ascorbate + O2
oxidized oligosaccharides + dehydroascorbate + H2O
-
-
-
?
beta-chitin + cellobiose dehydrogenase + O2
C1-oxidized oligosaccharides + reduced cellobiose dehydrogenase + H2O
cellobiose dehydrogenase from Myriococcum thermophilum can act as an electron donor
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-
?
beta-chitin + cellobiose dehydrogenase + O2
C1-oxidized oligosaccharides + reduced cellobiose dehydrogenase + H2O
cellobiose dehydrogenase from Myriococcum thermophilum can act as an electron donor
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-
?
chitin + acceptor + O2
C1-oxidized chito-oligosaccharides + reduced acceptor + H2O
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primary chain cleavage, yielding predominantly aldonic acid oligosaccharides with even-numbered degrees of polymerization plus significant presence of unmodified oligosaccharides with uneven-numbered degrees of polymerization
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?
chitin + acceptor + O2
C1-oxidized chito-oligosaccharides + reduced acceptor + H2O
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primary chain cleavage, yielding predominantly aldonic acid oligosaccharides with even-numbered degrees of polymerization plus significant presence of unmodified oligosaccharides with uneven-numbered degrees of polymerization
-
?
chitin + acceptor + O2
[chitin oligosaccharide]-N-acetyl-D-glucosaminate + [chitin oligosaccharide]-N-acetyl-D-glucosamino-1,5-lactone
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the products are a series of chitin oligosaccharides in aldonic acid or lactone form with varying degree of polymerization: DPox4 > DPox5 > DPox6 > DPox7 > DPox8. Oligosaccharides with even-numbered chain lengths are predominant.
-
?
chitin + acceptor + O2
[chitin oligosaccharide]-N-acetyl-D-glucosaminate + [chitin oligosaccharide]-N-acetyl-D-glucosamino-1,5-lactone
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the products are a series of chitin oligosaccharides in aldonic acid or lactone form with varying degree of polymerization: DPox4 > DPox5 > DPox6 > DPox7 > DPox8. Oligosaccharides with even-numbered chain lengths are predominant.
-
?
chitin + ascorbic acid + O2
? + dehydroascorbate + H2O
-
-
-
?
chitin + ascorbic acid + O2
? + dehydroascorbate + H2O
-
-
-
?
chitin + ascorbic acid + O2
? + dehydroascorbate + H2O
-
-
-
?
chitin + ascorbic acid + O2
? + dehydroascorbate + H2O
-
-
-
?
colloidal chitin + ascorbate + O2
? + dehydroascorbate + H2O
-
low activity, products show less dominance of even-numbered products than alpha- or beta-chitin
-
?
colloidal chitin + ascorbate + O2
? + dehydroascorbate + H2O
-
low activity, products show less dominance of even-numbered products than alpha- or beta-chitin
-
?
colloidal chitin + ascorbate + O2
oxidized chitin oligosaccharides + dehydroascorbate + H2O
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with colloidal chitin as the substrate, a ladder of oxidized oligosaccharides is observed
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?
colloidal chitin + ascorbate + O2
oxidized chitin oligosaccharides + dehydroascorbate + H2O
-
with colloidal chitin as the substrate, a ladder of oxidized oligosaccharides is observed
-
?
colloidal chitin + ascorbate + O2
[chitin oligosaccharide]-N-acetyl-D-glucosaminate + [chitin oligosaccharide]-N-acetyl-D-glucosamino-1,5-lactone + dehydroascorbate + H2O
-
-
-
?
colloidal chitin + ascorbate + O2
[chitin oligosaccharide]-N-acetyl-D-glucosaminate + [chitin oligosaccharide]-N-acetyl-D-glucosamino-1,5-lactone + dehydroascorbate + H2O
-
-
-
?
crystalline chitin + ascorbate + O2
oxidized oligosaccharides + dehydroascorbate + H2O
-
enzyme generates even-numbered oxidized oligosaccharides as the dominated products from crystalline chitin
-
?
crystalline chitin + ascorbate + O2
oxidized oligosaccharides + dehydroascorbate + H2O
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enzyme generates even-numbered oxidized oligosaccharides as the dominated products from crystalline chitin
-
?
phosphoric acid swollen cellulase + ascorbic acid + O2
? + dehydroascorbate + H2O
-
-
-
?
phosphoric acid swollen cellulase + ascorbic acid + O2
? + dehydroascorbate + H2O
-
-
-
?
phosphoric acid swollen cellulase + ascorbic acid + O2
? + dehydroascorbate + H2O
-
-
-
?
phosphoric acid swollen cellulase + ascorbic acid + O2
? + dehydroascorbate + H2O
-
-
-
?
[(1->4)-N-acetyl-beta-D-glucosaminyl]n+m + reduced acceptor + O2
[(1->4)-N-acetyl-beta-D-glucosaminyl]m-1-(1->4)-N-acetyl-2-deoxy-2-amino-D-glucono-1,5-lactone + [(1->4)-N-acetyl-beta-D-glucosaminyl]n + acceptor + H2O
-
-
-
?
[(1->4)-N-acetyl-beta-D-glucosaminyl]n+m + reduced acceptor + O2
[(1->4)-N-acetyl-beta-D-glucosaminyl]m-1-(1->4)-N-acetyl-2-deoxy-2-amino-D-glucono-1,5-lactone + [(1->4)-N-acetyl-beta-D-glucosaminyl]n + acceptor + H2O
-
-
-
?
additional information
?
-
no activity on other substrates including diverse mannans, cellulose and starch
-
-
?
additional information
?
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no activity on other substrates including diverse mannans, cellulose and starch
-
-
?
additional information
?
-
the enzyme produces oxidized chitin oligosaccharides with a degree of polymerization from DPox3 to DPox11. The relative intensities of DPox4, DPox6, DPox8 and DPox10 are remarkably higher than DPox5, DPox7, DPox9 and DPox11. LPMO10A shows little binding to Avicel
-
-
?
additional information
?
-
the enzyme produces oxidized chitin oligosaccharides with a degree of polymerization from DPox3 to DPox11. The relative intensities of DPox4, DPox6, DPox8 and DPox10 are remarkably higher than DPox5, DPox7, DPox9 and DPox11. LPMO10A shows little binding to Avicel
-
-
?
additional information
?
-
data indicate that catalysis involves equatorial binding of a reactive oxygen species
-
-
?
additional information
?
-
the enzyme is active on alpha- and beta-chitin, and the chitin-binding surface previously described for larger LPMOs is fully conserved
-
-
?
additional information
?
-
data indicate that catalysis involves equatorial binding of a reactive oxygen species
-
-
?
additional information
?
-
the enzyme is active on alpha- and beta-chitin, and the chitin-binding surface previously described for larger LPMOs is fully conserved
-
-
?
additional information
?
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enzyme binds to cellulose, but does not display catalyic activity
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-
?
additional information
?
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enzyme binds to cellulose, but does not display catalyic activity
-
-
?
additional information
?
-
mechanistic model, copper is reduced on the enzyme by an externally provided electron and followed by oxygen binding and activation by internal electron transfer. Substrate binding involves an extended planar binding surface, including the metal binding site. Chitin binding protects two regions from 2H/1H exchange, Gln53-Ser58 and Leu110-Thr116
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-
?
additional information
?
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[(1->4)-N-acetyl-beta-D-glucosaminyl]6 is a substrate, but not shorter oligomers
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-
?
additional information
?
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enzyme in presence of ascorbate but lacking chitin produces H2O2
-
-
?
additional information
?
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isoform LPMO10B produces C4-oxidized (4-ketoaldoses) and double (C4/C1)-oxidized cello-oligosaccharides. No substrate: alpha-chitin
-
-
?
additional information
?
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isoform LPMO10B produces C4-oxidized (4-ketoaldoses) and double (C4/C1)-oxidized cello-oligosaccharides. No substrate: alpha-chitin
-
-
?
additional information
?
-
enzyme in presence of ascorbate but lacking chitin produces H2O2
-
-
?
