1.14.99.29: deoxyhypusine monooxygenase

This is an abbreviated version!
For detailed information about deoxyhypusine monooxygenase, go to the full flat file.

Word Map on EC 1.14.99.29

1.14.99.29

eif-5a

spermidine

hypusine-containing

gc7

mimosine

ciclopirox

nepsilon-4-amino-2-hydroxybutyllysine

drug development

deferiprone

eif5a-1

polyamine-derived

4-aminobutyl

diiron

n1-guanyl-1,7-diaminoheptane

heat-repeats

heat-like

peroxo

deoxyhypusine-containing

medicine

Reaction

Synonyms

deoxyhypusine hydroxylase, deoxyhypusine hydroxylase homologue nero, deoxyhypusine synthase/hydroxylase, deoxyhypusyl hydroxylase, DOHH, DOOH, hDOHH, Lia1, More, nero, oxygenase, deoxyhypusine di-

ECTree

1 Oxidoreductases

1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen

1.14.99 Miscellaneous

1.14.99.29 deoxyhypusine monooxygenase

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Results	in table
3	Activating Compound
2952	AA Sequence
10	Application
2	CAS Registry Number
22	Cloned(Commentary)
2	Cofactor
1	Crystallization (Commentary)
19	Disease/ Diagnostics
1	Expression
50	General Information
3	General Stability
16	IC50 Value
103	Inhibitors
3	Ki Value [mM]
4	KM Value [mM]
2	Localization
25	Metals/Ions
10	Molecular Weight [Da]
37	Natural Substrates/ Products (Substrates)
51	Organism
1	Pathway
4	PDB ID
12	pH Optimum
3	pI Value
56	Protein Variants
16	Purification (Commentary)
2	Reaction
21	Reaction Type
51	Reference
1	Renatured (Commentary)
36	Source Tissue
6	Specific Activity [micromol/min/mg]
1	Storage Stability
67	Substrates and Products (Substrate)
21	Subunits
80	Synonyms
1	Systematic Name
10	Temperature Optimum [°C]

Purification

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Purification on EC 1.14.99.29 - deoxyhypusine monooxygenase

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2 entries

GSH-Sepharose resin column chromatography and Superdex 200 gel filtration

Homo sapiens

Q9BU89

745386

GST column chromatography and Superdex 75 gel filtration

Homo sapiens

Q9BU89

746515

native enzyme is partially purified from testis

Rattus norvegicus

713727

native plasmodial DOHH by anion exchange chromatography and nickel affinity chromatography

Plasmodium falciparum

713727

Ni-NTA column chromatography, and Superdex 200 gel filtration

Plasmodium falciparum

744002

partial

Rattus norvegicus

438280

performed by nickel-chelate affinity chromatography under native conditions

Plasmodium falciparum

C9QNK6

697912

recombinant GST-tagged DOHH by glutathione affinity chromatography

Saccharomyces cerevisiae

713727

recombinant GST-tagged DOHH by glutathione affinity chromatography. Recombinant His-tagged hypusine pathway enzymes from Escherichia coli by anion exchange and nickel affinity chromatography

Homo sapiens

713727

recombinant GST-tagged Lia1 from Escherichia coli strain BL21(DE3) by two steps of glutathione affinity chromatography and gel filtration. Separation of iron-free and iron-bound forms by gel filtration and native electrophoresis

Saccharomyces cerevisiae

713728

recombinant GST-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography, the tag is cleaved off by thrombin

Homo sapiens

674901

recombinant GST-tagged wild-type and mutant enzymes from Escherichia coli strain JM109 by glutathione affinity chromatography, the GST-tag is cleaved off

Bos taurus

Q0VC53

689862

recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affiniity chromatography

Plasmodium vivax

A5JZ19

728594