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1.14.99.22: ecdysone 20-monooxygenase

This is an abbreviated version!
For detailed information about ecdysone 20-monooxygenase, go to the full flat file.

Word Map on EC 1.14.99.22

Reaction

ecdysone
+
reduced acceptor
+
O2
=
20-hydroxyecdysone
+
acceptor
+
H2O

Synonyms

alpha-ecdysone C-20 hydroxylase, BmE20OHase, Cyp314A1, E-20-M, E20-M, E20M, E20MO, E20OHase, ecdysone 20-hydroxylase, ecdysone 20-monooxygenase, shade, shd

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.99 Miscellaneous
                1.14.99.22 ecdysone 20-monooxygenase

Purification

Purification on EC 1.14.99.22 - ecdysone 20-monooxygenase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
not: purification of the enzyme from microsomes failed due to unstability of the enzyme
-
recombinant GST-fusion protein
-
solubilization of the enzyme from microsomes with detergent Synperonic NP10
-