1.14.99.15: 4-methoxybenzoate monooxygenase (O-demethylating)

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For detailed information about 4-methoxybenzoate monooxygenase (O-demethylating), go to the full flat file.

Reaction

Synonyms

4-methoxybenzoate 4-monooxygenase (O-demethylating), 4-methoxybenzoate monooxygenase, 4-methoxybenzoate O-demethylase, CYP199A2, CYP199A4, cytochrome P450 199A2, oxygenase, 4-methoxybenzoate 4-mono- (O-demethylating), p-anisic O-demethylase, piperonylate-4-O-demethylase, RPA1871

ECTree

     1 Oxidoreductases

         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen

             1.14.99 Miscellaneous

                1.14.99.15 4-methoxybenzoate monooxygenase (O-demethylating)

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Results	in table
9	Activating Compound
25	AA Sequence
1	Application
1	CAS Registry Number
8	Cloned(Commentary)
26	Cofactor
3	Crystallization (Commentary)
11	General Information
6	General Stability
36	Inhibitors
37	KM Value [mM]
15	Metals/Ions
3	Molecular Weight [Da]
21	Natural Substrates/ Products (Substrates)
38	Organism
3	Oxidation Stability
2	Pathway
8	pH Optimum
1	pH Range
1	Posttranslational Modification
20	Protein Variants
8	Purification (Commentary)
4	Reaction
13	Reaction Type
21	Reference
55	Specific Activity [micromol/min/mg]
4	Storage Stability
127	Substrates and Products (Substrate)
4	Subunits
21	Synonyms
1	Systematic Name
12	Temperature Optimum [°C]
1	Turnover Number [1/s]

Crystallization

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Crystallization on EC 1.14.99.15 - 4-methoxybenzoate monooxygenase (O-demethylating)

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CRYSTALLIZATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

enzyme CYP199A2 bound to substrate 4-methoxybenzoate, X-ray diffraction structure determination and analysis at 1.8 A resolution

Rhodopseudomonas palustris

Q2IU02, Q6N8N2

727349

enzyme CYP199A4 free and bound to substrate 4-methoxybenzoate, hanging drop vapour diffusion method, for the free enzyme: mixing of 0.001 ml of protein solution containing 50 mg/ml protein in 20 mM HEPES, pH 7.4, 150 mM KCl, 1 mM DTT, with 0.001 ml of reservoir solution containing 0.1 M Bis-Tris, pH 5.5, 1.45-1.5 M ammonium sulfate, and 0.1 M sodium chloride, and equilibration against 0.2 ml of reservoir solution, 20°C, 1 week, for the substrate-bound enzyme: mixing of 0.001 ml of protein solution containing 40 mg/ml protein in 20 mM HEPES, pH 7.4, 150 mM KCl, and 10 mM 2-mercaptoethanol and saturated with 4-methoxybenzoate, with 0.001 ml of reservoir solution containing 0.1 M Bis-Tris, pH 5.5, 1.45 M ammonium sulfate, and 0.1 M sodium chloride, and equilibration against 0.2 ml of reservoir solution, 20°C, 2 weeks, X-ray diffraction structure determination and analysis at 2.6 A and 2.0 A resolution, respectively

Rhodopseudomonas palustris

Q2IU02, Q6N8N2

727349

purified recombinant CYP199A2, 16 ºC, the hanging drop vapor diffusion method under aerobic conditions, 0.0015 ml of protein solution is mixed with 0.0015 ml of reservoir solution, addition of 200 ml reservoir solution, containing 15% PEG 4000, 100 mM sodium citrate pH 5.6, 20% isopropanol with 4% v/v t-butanol, 1 week, X-ray diffraction structure deternnation and analysis at 2.0 A resolution

Rhodopseudomonas palustris

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