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1.14.99.15: 4-methoxybenzoate monooxygenase (O-demethylating)

This is an abbreviated version!
For detailed information about 4-methoxybenzoate monooxygenase (O-demethylating), go to the full flat file.

Word Map on EC 1.14.99.15

Reaction

4-Methoxybenzoate
+
reduced acceptor
 +
O2
=
4-hydroxybenzoate
 +
formaldehyde
 +
acceptor
 +
H2O

Synonyms

4-methoxybenzoate 4-monooxygenase (O-demethylating), 4-methoxybenzoate monooxygenase, 4-methoxybenzoate O-demethylase, CYP199A2, CYP199A4, cytochrome P450 199A2, oxygenase, 4-methoxybenzoate 4-mono- (O-demethylating), p-anisic O-demethylase, piperonylate-4-O-demethylase, RPA1871

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.99 Miscellaneous
                1.14.99.15 4-methoxybenzoate monooxygenase (O-demethylating)

Cloned

Cloned on EC 1.14.99.15 - 4-methoxybenzoate monooxygenase (O-demethylating)

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
CYP199A2 gene RPA1871, coexpression with palustris redoxin gene from Rhodopseudomonas palustris and putidaredoxin reductase gene from Pseudomonas putida to provide the redox partners of CYP199A2 in Escherichia coli
-
expressed in Escherichia coli BL21(DE3) cells
expression in Escherichia coli strain BL21(DE3)
expression of wild-type and mutant CYP199A2 in Escherichia coli strain BL21(DE3)
overexpression of the His-tagged enzyme in Escherichia coli BL21(DE3)
-
wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)