1.14.20.3: (5R)-carbapenem-3-carboxylate synthase
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For detailed information about (5R)-carbapenem-3-carboxylate synthase, go to the full flat file.
Reaction
Synonyms
carbapenem synthase, CarC
ECTree
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Metals Ions
Metals Ions on EC 1.14.20.3 - (5R)-carbapenem-3-carboxylate synthase
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Fe2+
Fe2+
Fe(II)-dependent oxygenase. An Fe(IV)-oxo intermediate abstracts the hydrogen (H*) from C5, and tyrosine 165, a residue not visualized in the published structures of CarC lacking bound substrate, donates H* to the opposite face of the resultant radical. The reaction oxidizes the Fe(II) cofactor to Fe(III), limiting wild-type CarC to one turnover, but substitution of the H-donating tyrosine disables stereoinversion and confers to CarC the capacity for catalytic substrate oxidation