1.14.20.1: deacetoxycephalosporin-C synthase
This is an abbreviated version!
For detailed information about deacetoxycephalosporin-C synthase, go to the full flat file.
Word Map on EC 1.14.20.1
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1.14.20.1
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clavuligerus
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chrysogenum
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acremonium
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isopenicillin
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penicillium
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beta-lactams
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epimerase
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cephamycins
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cephalosporium
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ring-expansion
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7-aminodeacetoxycephalosporanic
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pcbab
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ironii
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cephem
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lactamdurans
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synthesis
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7-adca
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2-oxoglutarate-dependent
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cephalexin
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carbenicillin
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doacs
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biotechnology
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medicine
- 1.14.20.1
- clavuligerus
- chrysogenum
- acremonium
- isopenicillin
- penicillium
- beta-lactams
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epimerase
-
cephamycins
- cephalosporium
-
ring-expansion
-
7-aminodeacetoxycephalosporanic
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pcbab
-
ironii
-
cephem
- lactamdurans
- synthesis
-
7-adca
-
2-oxoglutarate-dependent
- cephalexin
- carbenicillin
-
doacs
- biotechnology
- medicine
Reaction
Synonyms
acDAOC/DACS, cefE, cefEF, Cephalosporin biosynthesis expandase/hydroxylase, DAOC synthase, DAOC/DAC synthase, DAOC/DACS, DAOCS, deacetoxy/deacetylcephalosporin C synthase, deacetoxycephalosporin C synthase, deacetoxycephalosporin-C synthase, deacetoxycephalosporin-C synthetase, deacetoxycephalosporin/deacetylcephalosporin C synthase, expandase, expendase, penicillin N expandase, scDAOCS
ECTree
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Turnover Number
Turnover Number on EC 1.14.20.1 - deacetoxycephalosporin-C synthase
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0.005
pH 7.5, cosubstrate 2-oxo-4-methyl-pentanoate, recombinant mutant R258H
0.007
ampicillin
pH 7.5, cosubstrate 2-oxohexanoate, recombinant mutant R258H
0.008
ampicillin
pH 7.5, cosubstrate 2-oxohexanoate, recombinant mutant R258A
0.009
ampicillin
pH 7.5, cosubstrate 2-oxo-4-methyl-pentanoate, recombinant mutant R258L
0.009
ampicillin
pH 7.5, cosubstrate 2-oxohexanoate, recombinant mutant R258K
0.011
ampicillin
pH 7.5, cosubstrate 2-oxo-4-methyl-pentanoate, recombinant mutants R258A and R258F
0.011
ampicillin
pH 7.5, cosubstrate 2-oxoglutarate, recombinant mutant R258K
0.012
ampicillin
pH 7.5, cosubstrate 2-oxohexanoate, recombinant mutants R258L and R258F
0.013
ampicillin
pH 7.5, cosubstrate 2-oxo-4-methyl-pentanoate, recombinant mutant R258K
0.014
ampicillin
pH 7.5, cosubstrate 2-oxo-4-methyl-pentanoate, recombinant mutant R258Q
0.014
ampicillin
pH 7.5, cosubstrate 2-oxoglutarate, recombinant wild-type enzyme
0.017
ampicillin
pH 7.5, cosubstrate 2-oxohexanoate, recombinant mutant R258Q
0.001
pH 7.5, cosubstrate 2-oxohexanoate, recombinant mutant R258K
0.0057
penicillin G
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wild-type enzyme, pH not specified in the publication, temperature not specified in the publication
0.01
penicillin G
pH 7.5, cosubstrate 2-oxoglutarate, recombinant mutant R258K
0.01
penicillin G
pH 7.5, cosubstrate 2-oxohexanoate, recombinant mutant R258Q
0.013
penicillin G
pH 7.5, cosubstrate 2-oxohexanoate, recombinant mutants R258A and R258H
0.014
penicillin G
pH 7.5, cosubstrate 2-oxo-4-methyl-pentanoate, recombinant mutant R258H
0.019
penicillin G
pH 7.5, cosubstrate 2-oxohexanoate, recombinant mutant R258L
0.02
penicillin G
pH 7.5, cosubstrate 2-oxo-4-methyl-pentanoate, recombinant mutants R258K, R258A, and R258L
0.021
penicillin G
pH 7.5, cosubstrate 2-oxo-4-methyl-pentanoate, recombinant mutant R258Q
0.021
penicillin G
pH 7.5, cosubstrate 2-oxoglutarate, recombinant wild-type enzyme
0.024
penicillin G
pH 7.5, cosubstrate 2-oxohexanoate, recombinant mutant R258F
0.025
penicillin G
pH 7.5, cosubstrate 2-oxo-4-methyl-pentanoate, recombinant mutant R258F
0.0297
penicillin G
recombinant FF2 derived from gene shuffling
0.048
penicillin G
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mutant R308L, pH not specified in the publication, temperature not specified in the publication
0.054
penicillin G
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wild-type enzyme, pH and temperature not specified in the publication
0.054
penicillin G
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recombinant wild-type enzyme, pH not specified in the publication, 30°C
0.056
penicillin G
recombinant His-tagged enzyme, pH 7.5, 30°C
0.057
penicillin G
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recombinant mutant T213V, pH not specified in the publication, 30°C
0.064
penicillin G
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recombinant mutant S261A, pH not specified in the publication, 30°C
0.069
penicillin G
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mutant R308I, pH not specified in the publication, temperature not specified in the publication
0.07
penicillin G
30°C, triple mutant with truncation at residue 310, M306I and N305L
0.081
penicillin G
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recombinant mutant Y184A, pH not specified in the publication, 30°C
0.091
penicillin G
30°C, double mutant with truncation at residue 310 and M306I
0.225
penicillin G
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recombinant mutant Q126M, pH not specified in the publication, 30°C
0.23
penicillin G
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mutant C155Y/Y184H/V275I/C281Y, pH and temperature not specified in the publication
0.37
penicillin G
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mutant I305L, pH and temperature not specified in the publication
0.433
penicillin G
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recombinant mutant S261M, pH not specified in the publication, 30°C
0.537
penicillin G
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mutant I305M/S261M, pH and temperature not specified in the publication
0.589
penicillin G
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mutantI305M/T213V/M73T, pH and temperature not specified in the publication
0.65
penicillin G
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mutant I305M, pH and temperature not specified in the publication
0.654
penicillin G
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mutant I305M/T213V, pH and temperature not specified in the publication
0.668
penicillin G
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mutant I305M/T213V/S261M, pH and temperature not specified in the publication
0.02
Penicillin N
mutant enzyme DELTAI305-310, DELTAR306-310 and mutant DELTAR307-310