1.14.19.46: sn-1 linoleoyl-lipid 6-desaturase

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For detailed information about sn-1 linoleoyl-lipid 6-desaturase, go to the full flat file.

Reaction

+ 2 reduced ferredoxin [iron-sulfur] cluster +

+ 2 H+ =

a 1-gamma-linolenoyl-2-acyl-[glycerolipid]

+ 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O

Synonyms

DELTA6 desaturase, DELTA6-acyl-lipid desaturase, DELTA6-desaturase, desD

ECTree

1 Oxidoreductases

1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen

1.14.19 With oxidation of a pair of donors resulting in the reduction of O₂ to two molecules of water

1.14.19.46 sn-1 linoleoyl-lipid 6-desaturase

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Results	in table
2	Activating Compound
4	Cloned(Commentary)
6	Cofactor
2	KM Value [mM]
1	Localization
2	Molecular Weight [Da]
7	Natural Substrates/ Products (Substrates)
6	Organism
4	Pathway
14	Protein Variants
1	Reaction
6	Reference
7	Substrates and Products (Substrate)
2	Subunits
13	Synonyms
1	Systematic Name

Reference

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Reference on EC 1.14.19.46 - sn-1 linoleoyl-lipid 6-desaturase

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675896

Kurdrid, P.; Subudhi, S.; Hongsthong, A.; Ruengjitchatchawalya, M.; Tanticharoen, M.

Functional expression of Spirulina-DELTA6 desaturase gene in yeast, Saccharomyces cerevisiae

Mol. Biol. Rep.

215-226

2005

Arthrospira platensis

16328883

675898

Kurdrid, P.; Subudhi, S.; Cheevadhanarak, S.; Tanticharoen, M.; Hongsthong, A.

Effect of two intermediate electron donors, NADPH and FADH(2), on Spirulina DELTA6-desaturase co-expressed with two different immediate electron donors, cytochrome b5 and ferredoxin, in Escherichia coli

Mol. Biol. Rep.

261-266

2006

Arthrospira platensis

17160624

733180

Hongsthong, A.; Subudhi, S.; Sirijuntarat, M.; Cheevadhanarak, S.

Mutation study of conserved amino acid residues of Spirulina DELTA6-acyl-lipid desaturase showing involvement of histidine 313 in the regioselectivity of the enzyme

Appl. Microbiol. Biotechnol.

74-84

2004

Arthrospira platensis

15241633

733181

Hongsthong, A.; Subudhi, S.; Sirijuntarut, M.; Kurdrid, P.; Cheevadhanarak, S.; Tanticharoen, M.

Revealing the complementation of ferredoxin by cytochrome b5 in the Spirulina-DELTA6-desaturation reaction by N-terminal fusion and co-expression of the fungal-cytochrome b5 domain and Spirulina-DELTA6-acyl-lipid desaturase

Appl. Microbiol. Biotechnol.

1192-1201

2006

Arthrospira platensis

16575563

733373

Szalontai, B.; Nishiyama, Y.; Gombos, Z., Murata, N.

Membrane dynamics as seen by fourier transform infrared spectroscopy in a cyanobacterium, Synechocystis PCC 6803. The effects of lipid unsaturation and the protein-to-lipid ratio

Biochim. Biophys. Acta

1509

409-419

2000

Synechocystis sp.

11118550

733718

Tasaka, Y.; Gombos, Z.; Nishiyama, Y.; Mohanty, P.; Ohba, T.; Ohki, K.; Murata, N.

Targeted mutagenesis of acyl-lipid desaturases in Synechocystis: evidence for the important roles of polyunsaturated membrane lipids in growth, respiration and photosynthesis

EMBO J.

6416-6425

1996

Synechocystis sp.

8978669