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1.14.19.4: acyl-lipid (11-3)-desaturase

This is an abbreviated version!
For detailed information about acyl-lipid (11-3)-desaturase, go to the full flat file.

Word Map on EC 1.14.19.4

Reaction

an (11Z,14Z)-icosa-11,14-dienoyl-[glycerolipid]
+ 2 ferrocytochrome b5 +
O2
 + 2 H+ =
an (8Z,11Z,14Z)-icosa-8,11,14-trienoyl-[glycerolipid]
 + 2 ferricytochrome b5 + 2 H2O

Synonyms

AtSLD1, AtSLD2, Boofd8, Ca-SLDp, D8A, DELTA6-desaturase, DELTA8 acyl-CoA-dependent desaturase, DELTA8 desaturase, DELTA8 sphingobase desaturase, Delta8 sphingolipid desaturase, DELTA8-desaturase, DELTA8-fatty-acid desaturase, DELTA8-sphingolipid desaturase, FADS2, SLD, SlSLD, sphingolipid delta8 desaturase, sphingolipid DELTA8 LCB desaturase, sphingolipid DELTA8 long-chain base desaturase, sphingolipid DELTA8-desaturase, TpdesB

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.19 With oxidation of a pair of donors resulting in the reduction of O2 to two molecules of water
                1.14.19.4 acyl-lipid (11-3)-desaturase

Cloned

Cloned on EC 1.14.19.4 - acyl-lipid (11-3)-desaturase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloned into pBINI19-35S, transferred to Agrobacterium tumefaciens strain GV3101 and expressed in wild-type Arabidopsis Col 0 or in transgenic line CA1-9 expressing the Isochrysis galbana elongating activity IgASE1
-
cloned into the yeast expression vector pYES2 and expressed in Saccharomyces serevisiae strain W303-1A
-
expressed in yeast cells
-
expression in Saccharomyces cerevisiae
expression in Saccharomyces cerevisiae confers tolerance to aluminium to the cells
AF001394
expression of this open-reading frame in Saccharomyces cerevisiae results in the formation of DELTA-trans/cis-phytosphingenines not present in wild-type cells
functional expression in Saccharomyces cerevisiae, using the constitutive ADH1 promotor of the yeast expression vector pVT-U-102, results in synthesis of acylated (8E)- and (8Z)-4-hydroxy-8-sphingenine via N-acyl-4-hydroxysphinganine from fed deuterium-labeled palmitic acid, primary kinetic isotope effects, overview
-
gene AtSLD1, sequence comparisons, quantitative enzyme expression analysis, recombinant expression as cYFP fusion protein driven by cauliflower mosaic virus 35S (CaMV35S) promoter in onion epidermal cells, coexpression of AtBI-1-cYFP and AtSLD1-nYFP
gene AtSLD2, sequence comparions, quantitative enzyme expression analysis, recombinant expression as cYFP fusion protein driven by cauliflower mosaic virus 35S (CaMV35S) promoter in onion epidermal cells
gene S581, DNA and amino acid sequence determination and analysis, expression in Saccharomyces cerevisiae and constitutively in Arabidopsis thaliana, under control of the CaMV35S promoter, confers tolerance to aluminium and gadolinium, Gd3+, but not to lanthanum, La3+, or manganese, Mn2+, to cells and seedlings, respectively, the transgenic expression leads to the accumulation of 8(Z/E)-C18-phytosphingenine and 8(Z/E)-C20-phytopshingenine in yeast and to the accumulation of 8(Z/E)-C18-phytosphingenine in the leaves and roots of Arabidopsis plants, overview
genes SlSLD1 and SlSLD2, quantitative RT-PCR enzyme expression analysis
-
into pBluescript II SK+ and subsequently into pFLAG-Act1
-
into the pGEM-T easy vector for sequencing, into the pYES2 vector for expression in Saccharomyces cerevisiae cells