1.14.19.33: DELTA12 acyl-lipid conjugase (11E,13E-forming)
This is an abbreviated version!
For detailed information about DELTA12 acyl-lipid conjugase (11E,13E-forming), go to the full flat file.
Reaction
+ 2 ferrocytochrome b5 + + 2 H+ = + 2 ferricytochrome b5 + 2 H2O
Synonyms
DELTA12 desaturase, DELTA12 oleate desaturase, FADX, FADX-1A, FADX-1B, FADX-2, fatty acid DELTA12-conjugase
ECTree
Advanced search results
Engineering
Engineering on EC 1.14.19.33 - DELTA12 acyl-lipid conjugase (11E,13E-forming)
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
D115E
site-directed mutagenesis, the mutant shows reaction product like the wild-type enzyme, predominantly alpha-eleostearic acid and little punicic acid
G111V
site-directed mutagenesis, the mutant shows reaction product like the wild-type enzyme, predominantly alpha-eleostearic acid and little punicic acid
G111V/D115E
site-directed mutagenesis, the mutant shows reaction product unlike the wild-type enzyme, approximately equal amounts of alpha-eleostearic acidandits isomer, punicic acid
additional information
construction of a series of Momordica charantia FADX-Arabidopsis thaliana FAD2 chimeras: chimera 1 contains Momordica FADX amino acids 1-157 and Arabidopsis FAD2 amino acids 149-383, chimera 2 contains FADX aa 1210 with FAD2 aa 202-383, chimera 3 contains FADX aa 1-252 with FAD2 aa 244-383, chimera 4 contains FADX aa 1-326 with FAD2 aa 317-383, chimera 5 contains FAD2 aa 1-52 with FADX aa 61-399, chimera 6 contains FAD2 aa 1-83 with FADX aa 93-399, chimera 7 contains FAD2 aa 1-116 with FADX aa 126-399, and chimera 8 containsFAD2 aa 1-148 with FADX aa 158-399, mutant product formations, overview. Analysis of a FADX mutant containing six substitutions in which the sequence of helix 2 and first histidine box is converted to that of FAD2