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1.14.19.33: DELTA12 acyl-lipid conjugase (11E,13E-forming)

This is an abbreviated version!
For detailed information about DELTA12 acyl-lipid conjugase (11E,13E-forming), go to the full flat file.

Reaction

a gamma-linolenoyl-[glycerolipid]
+ 2 ferrocytochrome b5 +
O2
+ 2 H+ =
an alpha-parinaroyl-[glycerolipid]
+ 2 ferricytochrome b5 + 2 H2O

Synonyms

DELTA12 desaturase, DELTA12 oleate desaturase, FADX, FADX-1A, FADX-1B, FADX-2, fatty acid DELTA12-conjugase

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.19 With oxidation of a pair of donors resulting in the reduction of O2 to two molecules of water
                1.14.19.33 DELTA12 acyl-lipid conjugase (11E,13E-forming)

Cloned

Cloned on EC 1.14.19.33 - DELTA12 acyl-lipid conjugase (11E,13E-forming)

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Saccharomyces cerevisiae
expression in Saccharomyces cerevisiae and somatic embryos of Glycine max
expression in somatic embryos of Glycine max
gene FADX, combined transgenic expression of Punica granatum conjugase (FADX) under the control of a seed-specific napin promoter and Arabidopsis thaliana FAD2 desaturase, EC 1.14.19.34, in high linoleic acid Arabidopsis thaliana fad3/fae1 mutant, via Agrobacterium tumefaciens cell strain GV3101 transformation, leads to increased accumulation of punicic acid, quantitative RT-PCR enzyme expression analysis. Arabidopsis thaliana seeds overexpressing PgFADX show accumulation of punicic acid up to 9.2% average in tandem lines (6.43% in lines expressing FADX alone), highest content in line NCJ-11 is 11.26%, phenotypes, overview. Overexpression of PgFADX affects the activity of the native Arabidopsis thaliana FAD2 desaturase, the relative AtFAD2 expression level is significantly reduced up to 99%
gene FADX, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree, quantitative real-time PCR enzyme expression analysis, recombinant expression in Saccharomyces cerevisiae strains ole1 and INVSc1 in microsomes. Santalaceae FADX expressed in yeast has no detectable DELTA12 desaturase activity either acting on native fatty acids or when supplied with exogenous substrates but instead shows cis-DELTA13 desaturation activity on stearolic acid
gene FADX, DNA and amino acid sequence determination and analysis, phylogenetic analysis, functional expression of the enzyme in Saccharomyces cerevisiae strain MMYO11, transient expression of myc-tagged enzyme in Nicotiana tabacum cv. Bright-Yellow 2 suspension-cultured BY-2 cells
gene FADX, sequence comparisons, recombinant expression of enzyme mutant chimeras in the Arabidopsis thaliana fad3fae1 mutant strain, lacks the activity of both the endoplasmic reticulum omega-3 desaturase (FAD3) and the fatty acid elongation 1 (FAE1)-condensing enzyme, as well as elevated levels of linoleic acid
gene FADX-1A, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree, quantitative real-time PCR enzyme expression analysis, recombinant expression in Saccharomyces cerevisiae strains ole1 and INVSc1 in microsomes. Santalaceae FADX expressed in yeast has no detectable DELTA12 desaturase activity either acting on native fatty acids or when supplied with exogenous substrates but instead shows cis-DELTA13 desaturation activity on stearolic acid
gene FADX-1B, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree, quantitative real-time PCR enzyme expression analysis, recombinant expression in Saccharomyces cerevisiae strains ole1 and INVSc1 in microsomes. Santalaceae FADX expressed in yeast has no detectable DELTA12 desaturase activity either acting on native fatty acids or when supplied with exogenous substrates but instead shows cis-DELTA13 desaturation activity on stearolic acid
gene FADX-2, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree, quantitative real-time PCR enzyme expression analysis, recombinant expression in Saccharomyces cerevisiae strains ole1 and INVSc1 in microsomes. Santalaceae FADX expressed in yeast has no detectable DELTA12 desaturase activity either acting on native fatty acids or when supplied with exogenous substrates but instead shows cis-DELTA13 desaturation activity on stearolic acid