1.14.18.5: sphingolipid C4-monooxygenase
This is an abbreviated version!
For detailed information about sphingolipid C4-monooxygenase, go to the full flat file.
Word Map on EC 1.14.18.5
-
1.14.18.5
-
sphingolipids
-
sphingoid
-
phytosphingosine
-
phytoceramide
-
oxygen-dependent
-
inositolphosphorylceramide
-
c4-hydroxylation
-
nidulans
-
dihydrosphingosine
- 1.14.18.5
- sphingolipids
-
sphingoid
- phytosphingosine
- phytoceramide
-
oxygen-dependent
-
inositolphosphorylceramide
-
c4-hydroxylation
- nidulans
- dihydrosphingosine
Reaction
+ 2 ferrocytochrome b5 + + 2 H+ = + 2 ferricytochrome b5 +
Synonyms
bifunctional sphingolipid D4-desaturase/C4-hydroxylase, DES2, DHS hydroxylase, dihydroceramide hydrolase, dihydroceramide hydroxylase, EC 1.14.13.169, LmjF_26_1670, SBH1, SBH2, SC4H, sphinganine C4-hydroxylase, sphinganine C4-monooxygenase, sphingolipid C4-hydroxylase, sphingolipid delta(4)-desaturase/C4-monooxygenase, sphingolipid hydroxylase, SUR2, Sur2p, SYR2, Syr2p
ECTree
Advanced search results
Engineering
Engineering on EC 1.14.18.5 - sphingolipid C4-monooxygenase
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
additional information
the replacement of any one of conserved His residues of three histidine-rich motifs with an alanine eliminates hydroxylase activity in vivo and in vitro. Residues Phe 174, Asn 182, Ser 191, Leu 196, Pro 199, Asn 266, Tyr 269, Asp 271 and Gln 275 appear to be additionally important elements of the active site but their conversion into corresponding yeast DELTA7-sterol-C5(6)-desaturase Erg3p residues does not lead to a gain in desaturase activity
additional information
-
the replacement of any one of conserved His residues of three histidine-rich motifs with an alanine eliminates hydroxylase activity in vivo and in vitro. Residues Phe 174, Asn 182, Ser 191, Leu 196, Pro 199, Asn 266, Tyr 269, Asp 271 and Gln 275 appear to be additionally important elements of the active site but their conversion into corresponding yeast DELTA7-sterol-C5(6)-desaturase Erg3p residues does not lead to a gain in desaturase activity