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1.14.18.5: sphingolipid C4-monooxygenase

This is an abbreviated version!
For detailed information about sphingolipid C4-monooxygenase, go to the full flat file.

Word Map on EC 1.14.18.5

Reaction

a dihydroceramide
+ 2 ferrocytochrome b5 +
O2
+ 2 H+ =
a (4R)-4-hydroxysphinganine ceramide
+ 2 ferricytochrome b5 +
H2O

Synonyms

bifunctional sphingolipid D4-desaturase/C4-hydroxylase, DES2, DHS hydroxylase, dihydroceramide hydrolase, dihydroceramide hydroxylase, EC 1.14.13.169, LmjF_26_1670, SBH1, SBH2, SC4H, sphinganine C4-hydroxylase, sphinganine C4-monooxygenase, sphingolipid C4-hydroxylase, sphingolipid delta(4)-desaturase/C4-monooxygenase, sphingolipid hydroxylase, SUR2, Sur2p, SYR2, Syr2p

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.18 With another compound as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.18.5 sphingolipid C4-monooxygenase

Engineering

Engineering on EC 1.14.18.5 - sphingolipid C4-monooxygenase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D271T
98% of wild-type activity
F174L
complete loss of activity
H176A
complete loss of activity
H180A
complete loss of activity
H190A
complete loss of activity
H193A
complete loss of activity
H194A
complete loss of activity
H249A
complete loss of activity
H270A
complete loss of activity
H273A
complete loss of activity
H274A
complete loss of activity
L196W
complete loss of activity
N182P
complete loss of activity
N266G
complete loss of activity
P199C
complete loss of activity
Q275L
complete loss of activity
S191K
99% of wild-type activity
Y269C
complete loss of activity
additional information