1.14.18.4: phosphatidylcholine 12-monooxygenase
This is an abbreviated version!
For detailed information about phosphatidylcholine 12-monooxygenase, go to the full flat file.
Word Map on EC 1.14.18.4
-
1.14.18.4
-
triacylglycerols
-
ricinus
-
communis
-
bean
-
desaturase
-
purpurea
-
claviceps
-
phospholipase
-
fendleri
-
oilseed
-
linoleate
-
lesquerella
-
seed-specific
-
desaturation
-
lesquerolic
-
synthesis
-
petrochemical
-
18-carbon
-
condensing
- 1.14.18.4
- triacylglycerols
- ricinus
- communis
- bean
-
desaturase
- purpurea
-
claviceps
- phospholipase
- fendleri
-
oilseed
- linoleate
- lesquerella
-
seed-specific
-
desaturation
-
lesquerolic
- synthesis
-
petrochemical
-
18-carbon
-
condensing
Reaction
+ 2 ferrocytochrome b5 + + 2 H+ = + 2 ferricytochrome b5 +
Synonyms
castor DELTA12-hydroxylase, CFAH12, CFAH12 protein, CpFAH, CpFAH12, DELTA12 oleate hydroxylase, EC 1.14.13.26, FAH, FAH12, LFAH12, oleate 12-hydroxylase, oleate DELTA12-hydroxylase, oleate hydroxylase, oleoyl-12-hydroxylase, oxygenase, oleate DELTA12-mono, ricinoleic acid synthase
ECTree
Advanced search results
Substrates Products
Substrates Products on EC 1.14.18.4 - phosphatidylcholine 12-monooxygenase
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
REACTION DIAGRAM
1-acyl-2-oleoyl-sn-glycero-3-phosphocholine + ferrocytochrome b5 + O2
1-acyl-2-[(S)-12-hydroxyoleoyl]-sn-glycero-3-phosphocholine + ferricytochrome b5
1-acyl-2-oleoyl-sn-glycero-3-phosphocholine + ferrocytochrome b5 + O2
1-acyl-2-[(S)-12-hydroxyoleoyl]-sn-glycero-3-phosphocholine + ferricytochrome b5 + H2O
-
-
-
-
?
a 1-acyl-2-oleoyl-sn-glycero-3-phosphocholine + 2 ferrocytochrome b5 + O2 + 2 H+
a 1-acyl-2-ricinoleoyl-sn-glycero-3-phosphocholine + 2 ferricytochrome b5 + H2O
-
-
-
-
?
a 1-acyl-2-oleoyl-sn-glycero-3-phosphoethanolamine + 2 ferrocytochrome b5 + O2 + 2 H+
a 1-acyl-2-ricinoleoyl-sn-glycero-3-phosphoethanolamine + 2 ferricytochrome b5 + H2O
-
-
-
-
?
cis-linoleic acid + reduced acceptor + O2
densipolic acid + acceptor + H2O
-
-
-
?
eicosenoic acid + reduced acceptor + O2
lesquerolic acid + acceptor + H2O
-
-
-
?
linoleic acid + ferrocytochrome P-450 + O2
linolenic acid + ferricytochrome P-450 + H2O
-
-
-
-
?
oleic acid + 2 ferrocytochrome b5 + O2 + 2 H+
ricinoleic acid + 2 ferricytochrome b5 + H2O
oleic acid esterified to membrane lipid phosphatidylcholine + reduced acceptor + H+ + O2
hydroxy fatty acids in triacylglycerol + acceptor + H2O
D2CPE1
-
-
-
?
palmitoleate + ferrocytochrome P-450 + H+ + O2
12-hydroxypalmitoleate + ferricytochrome P-450 + H2O
-
-
-
-
?
1-acyl-2-[(S)-12-hydroxyoleoyl]-sn-glycero-3-phosphocholine + ferricytochrome b5
-
-
-
?
1-acyl-2-oleoyl-sn-glycero-3-phosphocholine + ferrocytochrome b5 + O2
1-acyl-2-[(S)-12-hydroxyoleoyl]-sn-glycero-3-phosphocholine + ferricytochrome b5
-
-
-
?
ricinoleic acid + acceptor + H2O
-
-
-
?
cis-oleic acid + reduced acceptor + O2
ricinoleic acid + acceptor + H2O
-
-
-
?
cis-oleic acid + reduced acceptor + O2
ricinoleic acid + acceptor + H2O
-
-
-
?
cis-oleic acid + reduced acceptor + O2
ricinoleic acid + acceptor + H2O
Ricinus communis OLE-1
-
-
-
?
12-hydroxy-oleate + ferricytochrome P-450 + H2O
-
-
-
-
?
oleate + ferrocytochrome P-450 + H+ + O2
12-hydroxy-oleate + ferricytochrome P-450 + H2O
the enzyme is primarily a fatty acid hydroxylase, rather than a bifunctional oleate 12-hydroxylase-desaturase, involved in ricinoleic acid biosynthesis, overview
-
-
?
oleate + ferrocytochrome P-450 + H+ + O2
12-hydroxy-oleate + ferricytochrome P-450 + H2O
the enzyme is primarily a fatty acid hydroxylase rather than a bifunctional oleate 12-hydroxylase-desaturase, overview
-
-
?
ricinoleic acid + 2 ferricytochrome b5 + H2O
-
-
-
?
oleic acid + 2 ferrocytochrome b5 + O2 + 2 H+
ricinoleic acid + 2 ferricytochrome b5 + H2O
-
-
i.e. 12-hydroxy-octadeca-cis-9-enoic acid
-
?
?
-
-
biosynthesis of ricinoleic acid in Claviceps purpurea is catalyzed by the fungal desaturase-like hydroxylase
-
-
?
additional information
?
-
-
the enzyme acts predominantly as a hydroxylase, introducing hydroxyl groups at the 12-position of oleic acid and palmitoleic acid. It also shows DELTA12 desaturase activities on 16C and 18C monounsaturated fatty acids and, to a much lesser extent, omega3 desaturase activities on ricinoleic acid, overview
-
-
?
additional information
?
-
in Lesquerella fendleri ricinoleic acid is efficiently elongated to lesquerolic acid in a reaction catalyzed by a condensing enzyme specific to hydroxy fatty acids
-
-
?
additional information
?
-
-
the activitiy of oleoyl-12-hydroxylase seems unaffected by the fatty acid at the sn-1 position of 1-acyl-2-oleoyl-sn-glycero-3-phosphocholine. 1-acyl-2-ricinoleoyl-sn-glycero-3-phosphocholine is formed mostly by the hydroxylation of 1-acyl-2-oleoyl-sn-glycero-3-phosphocholine, not from the incorporation of ricinoleate into 1-acyl-2-ricinoleoyl-sn-glycero-3-phosphocholine, and 1-acyl-2-oleoyl-sn-glycero-3-phosphoethanolamine is less actively formed than 1-acyl-2-oleoyl-sn-glycero-3-phosphocholine
-
-
?