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1.14.18.4: phosphatidylcholine 12-monooxygenase

This is an abbreviated version!
For detailed information about phosphatidylcholine 12-monooxygenase, go to the full flat file.

Word Map on EC 1.14.18.4

Reaction

a 1-acyl-2-oleoyl-sn-glycero-3-phosphocholine
+ 2 ferrocytochrome b5 +
O2
 + 2 H+ =
a 1-acyl-2-[(12R)-12-hydroxyoleoyl]-sn-glycero-3-phosphocholine
 + 2 ferricytochrome b5 +
H2O

Synonyms

castor DELTA12-hydroxylase, CFAH12, CFAH12 protein, CpFAH, CpFAH12, DELTA12 oleate hydroxylase, EC 1.14.13.26, FAH, FAH12, LFAH12, oleate 12-hydroxylase, oleate DELTA12-hydroxylase, oleate hydroxylase, oleoyl-12-hydroxylase, oxygenase, oleate DELTA12-mono, ricinoleic acid synthase

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.18 With another compound as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.18.4 phosphatidylcholine 12-monooxygenase

Cloned

Cloned on EC 1.14.18.4 - phosphatidylcholine 12-monooxygenase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
encoded enzyme has both hydroxylase and desaturase activities
expression in Arabidopsis thaliana
expression in Pichia pastoris
expression in Schizosaccharomyces pombe
-
FAH gene, unrooted phylogenetic analysis, functional expression in Saccharomyces cerevisiae, expression in transgenic Arabidopsis thaliana plants under control of a seed-specific promoter
-
fatty acyl desaturase homolog
-
gene FAH12, recombinant overexpression in Schizosaccharomyces pombe strain ARC010-1, RT-PCR enzyme expression analysis. The enzyme product is toxic for the cells. Gene plg7, encoding phospholipase A2, acts as a multicopy suppressor restoring the growth defect by removing ricinoleic acid from phospholipids and inducing secretion of a part of the released free ricinoleic acid into culture media
gene FAH12, subcloning in Escherichia coli strain DH5alpha, expression in Saccharomyces cerevisiae results in the synthesis of ricinoleic acid and very low levels of di-unsaturated fatty acids. Expression of LlinFAH12 in Arabidopsis thaliana seeds results in hydroxy fatty acid accumulation, fatty acid composition in transformed compared to wild-type plants, overview
gene RcFAH12, DNA and amino acid sequence determination and analysis, sequence comparisons, quantitative real-time PCR enzyme expression analysis, recombinant expression of wild-type and mutant enzymes in Saccharomyces cerevisiae strain W303-1A, fatty acid analysis
PCR-amplification, plant transformation by heat shock transformation of Agrobacterium tumefaciens (GV3101) with vector, expression in EMS mutant or T-DNA insertion lines of Arabidopsis thaliana deficient in the production of the enzymes diacylglycerol acyltransferase-1 (DGAT1), phospholipid: diacylglycerol acyltransferase-1 (PDAT1) and the homologue of PDAT1 phospholipid: diacylglycerol acyltransferase-2 (PDAT2)
D2CPE1