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1.14.18.3: methane monooxygenase (particulate)

This is an abbreviated version!
For detailed information about methane monooxygenase (particulate), go to the full flat file.

Word Map on EC 1.14.18.3

Reaction

methane
+
quinol
 +
O2
=
methanol
 +
quinone
 +
H2O

Synonyms

copper-containing membrane monooxygenase, copper-containing membrane-bound monooxygenase, CuMMO, membrane-associated methane monooxygenase, membrane-bound methane monooxygenase, membrane-embedded methane monooxygenase, methane hydroxylase, mMMO, MMO, particulate methane mono-oxygenase, particulate methane monooxygenas, particulate methane monooxygenase, particulate methane monooxygenase A, particulate methane-oxidizing complex, particulate MMO, PMH, pMMO, pMMO hydroxylase, pMMO-H, pMMO1, pMMO2, PmoA, PmoB, sMMO, soluble methane monooxygenase, spmoB

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.18 With another compound as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.18.3 methane monooxygenase (particulate)

Cloned

Cloned on EC 1.14.18.3 - methane monooxygenase (particulate)

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination and anaylsis, the gene is organized in the structural gene cluster pmoCAB, stable functional expression of the membrane-bound enzyme in Rhodococcus erythropolis strain LSSE8-1 by using the dsz promoter and ethane as the sole carbon source, method optimization, overview
-
expressed in Escherichia coli
expressed in Escherichia coli BL21 cells
-
expressed in Escherichia coli BL21(DE3) or Rosetta (DE3) pLysS cells
-
gene pmoA, design of a highly degenerate primer targeting copper-containing membrane-bound monooxygenase genes for community analysis of methane- and ammonia-oxidizing bacteria, two-step PCR strategy employing a tagged highly degenerate primer (THDP), designated THDP-PCR, method development and optimization, overview. DNA and amino acid sequence determination and analysis, phylogenetic analysis
genetic analysis of pMMO genes
-
pmoA cloned in pGEM-T Easy vector and transformed into Escherichia coli JM109 high-efficiency competent cells
recombinant expression of wild-type holoenzyme and truncated recombinant periplasmic domains of pMMO (spmoB)
two aqueous-exposed subdomains toward the N- and C-termini of the large subunit are expressed in Escherichia coli BL21 (DE3) cells
-