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1.14.18.1: tyrosinase

This is an abbreviated version!
For detailed information about tyrosinase, go to the full flat file.

Word Map on EC 1.14.18.1

Reaction

2 L-dopa +

O2
= 2 dopaquinone + 2 H2O

Synonyms

AbPPO1, AbPPO4, AbTYR, aurone synthase, catalase-phenol oxidase, catechol oxidase, catecholase, CATPO, chlorogenic acid oxidase, chlorogenic oxidase, cresolase, cresolase/monophenolase, CsPPO, CZA14Tyr, deoxy-tyrosinase, dihydroxy-L-phenylalanine:oxygen oxidoreductase, Diphenol oxidase, diphenolase, dopa oxidase, EC 1.10.3.1, EC 1.14.17.2, Hc-derived phenoloxidase, Hc-phenoloxidase, HcPO, HdPO, hemocyanin-derived phenoloxidase, jrPPO1, jrTYR, L-DOPA monophenolase, L-DOPA oxidase, L-DOPA:oxygen oxidoreductase, L-tyrosine hydroxylase, MdPPO1, melC2, MelC2 tyrosinase, met-tyrosinase, monophenol dihydroxyphenylalanine:oxygen oxidoreductase, monophenol monooxidase, monophenol monooxygenase, monophenol monoxygenase, monophenol oxidase, monophenol oxygen oxidoreductase, monophenol, 3,4-dihydroxy L-phenylalanine (L-DOPA):oxygen oxidoreductase, monophenol, dihydroxy-L-phenylalanine oxygen oxidoreductase, monophenol, dihydroxy-L-phenylalanine:oxygen oxidoreductase, monophenol, dihydroxyphenylalanine:oxygen oxidoreductase, monophenol, L-Dopa: oxidoreductase, monophenol, L-DOPA: oxygen oxidoreductase, monophenol, o-diphenol: oxygen oxidoreductase, monophenol, o-diphenol:O2 oxidoreductase, monophenol, o-diphenol:oxygen oxido-reductase, monophenol, o-diphenol:oxygen oxidoreductase, monophenol, polyphenol oxidase, monophenol: dioxygen oxidoreductases, hydroxylating, monophenolase, monphenol mono-oxygenase, More, mTyr, murine tyrosinase, mushroom tyrosinase, mushroom tyrosine, N-acetyl-6-hydroxytryptophan oxidase, o-diphenol oxidase, o-diphenol oxidoreductase, o-diphenol oxygen oxidoreductase, o-diphenol: O2 oxidoreductase, o-diphenol: oxidoreductase, o-diphenol:O2 oxidoreductase, o-diphenol:oxygen oxidoreductase, o-diphenolase, OCA1, Orf13, oxygen oxidoreductase, phenol oxidase, phenol oxidases, phenolase, phenoloxidase, PO, polyaromatic oxidase, polyphenol oxidase, polyphenol oxidase 3, polyphenol oxidase 4, polyphenol oxidase B, polyphenolase, polyphenoloxidase, PotPPO, PPO, PPO 3, PPO B, PPO1, PPO2, PPO3, pro-PO III, prophenoloxidase III, pyrocatechol oxidase, SPRTyr, ST94, ST94t, tryosinase, tryrosinase, TY, tyr, TYR1, TYR2, tyrA, TyrBm, tyrosinase, tyrosinase 2, tyrosinase 4, tyrosinase diphenolase, tyrosine-dopa oxidase

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.18 With another compound as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.18.1 tyrosinase

Molecular Weight

Molecular Weight on EC 1.14.18.1 - tyrosinase

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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
110000
dynamic light scattering
115000
116000 - 128000
Mushroom
-
-
118600 - 119500
-
beta-tyrosinase, sedimentation equilibrium
118800
-
gamma-tyrosinase, sedimentation equilibrium
12000 - 60000
Musa cavendishii
-
gel filtration, multiple forms
120000
122500
Mushroom
-
gel filtration
123800
Mushroom
-
alpha, beta and gamma isoenzymes, sedimetation equilibrium
13400
134000
-
2 * 134000 + 2 * 43000, alpha2beta2 subunit composition
14000
140200
ST94, MALDI-TOF mass spectrometry
15000
150000
160000
-
native PAGE, staining with L-beta-(3,4-dihydroxylphenyl)alanine
175000
-
1 * 175000, SDS-PAGE
175000 - 230000
-
SDS-PAGE in the presence of urea
188000
-
major peak, minor peak at 49000 Da, gel filtration
20000
-
the purified preparation gives three bands, 1 * 20000 + 1 * 27000 + 1 * 47000, SDS-PAGE
200000
-
high molecular weight L-DOPA-positive band of the recombinant enzyme, SDS-PAGE
220000 - 229000
-
gel filtration in the absence and presence of SDS
23500
-
3 * 30000 + 1 * 23500, SDS-PAGE
26000
-
x * 26000, SDS-PAGE
27000
-
the purified preparation gives three bands, 1 * 20000 + 1 * 27000 + 1 * 47000, SDS-PAGE
28800
-
gel filtration combined with multiangle laser light scattering
29000
-
x * 29000, intra- and extracellular enzyme, SDS-PAGE
29000 - 36000
-
gel filtration, SDS-PAGE
29500
-
gel filtration
30000
300000
-
gel filtration
30610
-
deduced from nucleotide sequence
30900
31000
32000
320000
-
SDS-PAGE
33000
-
x * 33000, isoenzyme 1 and 2, SDS-PAGE
330000
-
gel filtration
33600
-
1 * 33600, calculated from amino acid sequence
34000
-
sedimentation analysis
35000
36000
37900
-
1 * 37900, SDS-PAGE
38000
38500
Vibrio tyrosinaticus
-
isoenzyme B, gel filtration
38890
truncated enzyme form 2, comprising residues 101-445, HPLC/mass spectrometry
39000
39047
truncated enzyme form 1, comprising residues 101-445, HPLC/mass spectrometry
40000
41000
41300
-
gel filtration
42000
43000
43200
-
x * 43200, recombinant enzyme, SDS-PAGE, x * 61 151, unprocessed enzyme, sequence calculation, 43 124-43 204, recombinant enzyme, mass spectrometry
43500
-
determined by mass spectrometry
44000 - 47000
two protein bands (approximately 44-47 kDa) appear in transgenic alfalfa expressing PPO3
440000
-
gel filtration
46000
47000
-
the purified preparation gives three bands, 1 * 20000 + 1 * 27000 + 1 * 47000, SDS-PAGE
48000
-
x * 48000, enzyme form B, SDS-PAGE
49000
-
x * 54000 + x * 49000, isoenzymes A1 and A2, SDS-PAGE
50000
51300
-
SDS-PAGE
52000
-
1 * 52000 + 1 * 38000, the electrophoretic pattern of cherry PPO in SDS-PAGE reveals two dominant bands with molecular masses of 52 and 38 kDa
54000
54800
-
gel filtration
55000
56200
-
x * 56200, mature enzyme, deduced from amino acid sequence
56800
isozyme TYR2, calculated from amino acid sequence
57000
-
gel filtration
57300
-
x * 57300, estimated from SDS-PAGE
57500
-
gel filtration
58000
60000
62000
-
1 * 62000, SDS-PAGE
63000
-
1 * 63000 + 1 * 68000, SDS-PAGE
63800
2 * 63800, ST94t
65000
66000
66700
67000
67810
-
calculated
68000
69000
-
tyrosinase A and B, PAGE
70000
75000
78000
-
6 * 78000, SDS-PAGE
80000
90000
additional information