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1.14.17.4: aminocyclopropanecarboxylate oxidase

This is an abbreviated version!
For detailed information about aminocyclopropanecarboxylate oxidase, go to the full flat file.

Word Map on EC 1.14.17.4

Reaction

1-aminocyclopropane-1-carboxylate
+
ascorbate
 +
O2
=
Ethene
 +
cyanide
 +
dehydroascorbate
 +
CO2
 + 2 H2O

Synonyms

1-aminocyclopropane-1-carboxylate oxidase, 1-aminocyclopropane-1-carboxylic acid oxidase, 1-aminocyclopropane-1-carboxylic acid oxidase (gene CM-ACO1), 1-aminocyclopropane-1-carboxylic oxidase, 1-aminocyclopropyl-1-carboxylic acid oxidase, ACC oxidase, ACC oxidase 1, ACC oxidase 2, ACC-oxidase, ACCO, ACCO1, ACO, Aco1, Aco2, Aco3, ACO4, ACO6, aminocyclopropane-1-carboxylate oxidase, aminocyclopropane-2-carboxylic oxidase, AsACO, AtACO1, ethylene-forming enzyme, LeACO1, LeACO4, Ls-ACO1, Ls-ACO2, Ls-ACO3, MD-ACO1, MD-ACO2, MD-ACO3, NsACO, oxidase, aminocyclopropanecarboxylate, PgACO, PmACO, PsACO, TR-ACO1p, TR-ACO2p, TR-ACO3p, TR-ACO4p, ZmACO

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.17 With reduced ascorbate as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.17.4 aminocyclopropanecarboxylate oxidase

Specific Activity

Specific Activity on EC 1.14.17.4 - aminocyclopropanecarboxylate oxidase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.000000064
recombinant His-tagged mutant R175E/T157A, pH 7.2, 20°C
0.0000024
recombinant His-tagged mutant R175E/R244K, pH 7.2, 20°C
0.0000029
recombinant His-tagged mutant R244K/T157A, pH 7.2, 20°C
0.0000033
recombinant His-tagged mutant K158E, pH 7.2, 20°C
0.0000083
recombinant His-tagged mutant R299H, pH 7.2, 20°C
0.00001
recombinant His-tagged mutant S246A/R244K/T157A, pH 7.2, 20°C
0.000012
recombinant His-tagged mutant K158Q/R175Q, pH 7.2, 20°C
0.000021
recombinant His-tagged mutant K158Q/R175E, pH 7.2, 20°C
0.00004
recombinant His-tagged mutant R299L, pH 7.2, 20°C
0.000061
recombinant His-tagged mutant R175E/S246A, pH 7.2, 20°C
0.00007
recombinant His-tagged mutant K158L, pH 7.2, 20°C
0.000083
recombinant His-tagged mutant R175Q, pH 7.2, 20°C
0.00011
recombinant His-tagged mutant K158Q, pH 7.2, 20°C
0.00015
0.00017
recombinant His-tagged mutant R175G, pH 7.2, 20°C
0.0002
recombinant His-tagged mutant T157A, pH 7.2, 20°C
0.00022
recombinant His-tagged mutant K292E, pH 7.2, 20°C
0.00024
recombinant His-tagged mutant R175E, pH 7.2, 20°C
0.00025
recombinant His-tagged mutant K158R, pH 7.2, 20°C
0.00028
recombinant His-tagged mutant R175A, pH 7.2, 20°C
0.00065
recombinant His-tagged mutant R175K, pH 7.2, 20°C
0.00092
recombinant His-tagged mutant R244K/S246A, pH 7.2, 20°C
0.00116
recombinant His-tagged mutant R244K, pH 7.2, 20°C
0.00128
recombinant His-tagged mutant K292R, pH 7.2, 20°C
0.00147
recombinant His-tagged mutant R299K, pH 7.2, 20°C
0.00174
recombinant His-tagged mutant S246A, pH 7.2, 20°C
0.003
0.00348
recombinant His-tagged wild-type enzyme, pH 7.2, 20°C
0.0047
-
crude enzyme extract
0.006
-
pear fruit prior homogenization
0.008
-
at 10 mM L-alanine, purified recombinant enzyme
0.011
-
purified enzyme
0.012
partially purified recombinant isozyme ACO3
0.02
-
purified enzyme
0.023
-
at 10 mM D-alanine, purified recombinant enzyme
0.025
partially purified recombinant isozyme ACO1
0.035
-
purified enzyme
0.051
-
at 10 mM alpha-aminoisobutyric acid, purified recombinant enzyme
0.059
-
at 10 mM D-alpha-aminobutyric acid, purified recombinant enzyme
0.073
purified recombinant mutant K172C
0.1
purified recombinant mutant K172A
0.11
purified recombinant mutant G137P
0.13
-
at 10 mM 1-aminocyclopropane-1-carboxylate, purified recombinant enzyme
0.16
purified recombinant wild-type enzyme
additional information