1.14.17.3: peptidylglycine monooxygenase

This is an abbreviated version!
For detailed information about peptidylglycine monooxygenase, go to the full flat file.

Reaction

Synonyms

alpha-AE, bifunctional PAM, bifunctional peptidylglycine alpha-amidating monooxygenase, CG3832, hPHMcc, More, PAM, PAM-1, PAM-2, PAM-A, PAM-B, PAM/PHM, peptide alpha-amidating enzyme, peptide alpha-amide synthase, peptide-alpha-amide synthetase, peptidyl alpha-amidating enzyme, peptidyl alpha-hydroxylating monooxygenase, peptidyl-glycine alpha-amidating monooxygenase, peptidylglycine 2-hydroxylase, peptidylglycine alpha-amidating mono-oxygenase, peptidylglycine alpha-amidating monooxygenase, peptidylglycine alpha-hydroxylase, peptidylglycine alpha-hydroxylating monooxygenase, peptidylglycine alpha-hydroxylating-monooxygenase, peptidylglycine alpha-monooxygenase, peptidylglycine monooxygenase, peptidylglycine-alpha-amidating monooxygenase, PHM, PHMcc, synthase, peptide alpha-amide, type A PAM

ECTree

     1 Oxidoreductases

         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen

             1.14.17 With reduced ascorbate as one donor, and incorporation of one atom of oxygen into the other donor

                1.14.17.3 peptidylglycine monooxygenase

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Results	in table
6	Activating Compound
644	AA Sequence
9	Application
1	CAS Registry Number
43	Cloned(Commentary)
70	Cofactor
2	Crystallization (Commentary)
24	Disease/ Diagnostics
1	Expression
24	General Information
4	General Stability
24	IC50 Value
162	Inhibitors
1	kcat/KM [mM/s]
79	Ki Value [mM]
168	KM Value [mM]
43	Localization
81	Metals/Ions
37	Molecular Weight [Da]
83	Natural Substrates/ Products (Substrates)
101	Organism
3	Oxidation Stability
31	PDB ID
48	pH Optimum
5	pH Range
1	pH Stability
2	Posttranslational Modification
47	Protein Variants
44	Purification (Commentary)
30	Reaction
29	Reaction Type
99	Reference
116	Source Tissue
52	Specific Activity [micromol/min/mg]
372	Substrates and Products (Substrate)
19	Subunits
193	Synonyms
1	Systematic Name
31	Temperature Optimum [°C]
1	Temperature Stability [°C]
30	Turnover Number [1/s]

Natural Substrates Products

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Natural Substrates Products on EC 1.14.17.3 - peptidylglycine monooxygenase

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NATURAL SUBSTRATE

NATURAL PRODUCT

REACTION DIAGRAM

ORGANISM

UNIPROT

COMMENTARY
(Substrate)

LITERATURE
(Substrate)

COMMENTARY
(Product)

LITERATURE
(Product)

REVERSIBILITY
r=reversible
ir=irreversible
?=not specified

calcitonin + ascorbate + O2

? + dehydroascorbate + H2O

Rattus norvegicus

P14925

-

745298

-

-

?

glutathione + ascorbate + O2

gamma-Glu-Cys-NH2 + glyoxylate + dehydroascorbate + H2O

Rattus norvegicus

-

-

657649

-

-

?

insulin + ascorbate + O2

? + dehydroascorbate + H2O

Homo sapiens

P19021

-

746370

-

-

?

oxytoxin + ascorbate + O2

? + dehydroascorbate + H2O

Rattus norvegicus

P14925

-

745298

-

-

?

peptidyl-glycine + ascorbate + O2

peptidyl-(2-hydroxylglycine) + dehydroascorbate + H2O

Rattus norvegicus

-

-

687277

-

-

?

peptidylglycine + ascorbate + O2

peptidyl alpha-hydroxyglycine + semidehydroascorbate + H2O

2 entries

peptidylglycine + ascorbate + O2

peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O

44 entries

peptidylglycine + ascorbate + O2

peptidyl(2-hydroxylglycine) + dehydroascorbate + H2O

Rattus norvegicus

-

peptidylglycine alpha-hydroxylating monooxygenase reaction

658977

the carbinol is the substrate for the peptidylamidoglycolate lyase reaction, EC 4.3.2.5, forming glyoxylate and amidated peptide

-

?

proopiomelanocortin peptide + ascorbate + O2

proopiomelanocortin peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O

2 entries

R-CO-NH-CH2-COOH + ascorbate + O2

R-CO-NH2 + CHO-COOH + dehydroascorbate + H2O

Rattus norvegicus

-

-

657649

-

-

?

substance P + ascorbate + O2

? + dehydroascorbate + H2O

Rattus norvegicus

P14925

-

745298

-

-

?

thyrotropin + ascorbate + O2

? + dehydroascorbate + H2O

Rattus norvegicus

P14925

-

745298

-

-

?

additional information

?

-

18 entries

peptidylglycine + ascorbate + O2

peptidyl alpha-hydroxyglycine + semidehydroascorbate + H2O

Acropora millepora

J9QK52, J9QL63

-

728274

-

-

?

peptidylglycine + ascorbate + O2

peptidyl alpha-hydroxyglycine + semidehydroascorbate + H2O

Homo sapiens

-

-

726958

-

-

?

peptidylglycine + ascorbate + O2

peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O

Aplysia californica

-

-

438586

-

438586

?

peptidylglycine + ascorbate + O2

peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O

Bos taurus

-

-

438568

-

438568

?

peptidylglycine + ascorbate + O2

peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O

Bos taurus

-

-

438570

-

438570

?

peptidylglycine + ascorbate + O2

peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O

Bos taurus

-

-

438575

-

438575

?

peptidylglycine + ascorbate + O2

peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O

Bos taurus

-

-

438578

-

438578

?

peptidylglycine + ascorbate + O2

peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O

Bos taurus

-

-

438582

-

438582

?

peptidylglycine + ascorbate + O2

peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O

Bos taurus

-

-

438592

-

438592

?

peptidylglycine + ascorbate + O2

peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O

Bos taurus

-

COOH-terminal glycine

438567

-

438567

?

peptidylglycine + ascorbate + O2

peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O

Conus bullatus

-

-

728832

-

-

?

peptidylglycine + ascorbate + O2

peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O

Conus geographus

-

-

728832

-

-

?

peptidylglycine + ascorbate + O2

peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O

Equus caballus

-

-

438580

-

438580

?

peptidylglycine + ascorbate + O2

peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O

Homo sapiens

-

-

674660, 699036, 727016, 727146, 728237

-

-

?

peptidylglycine + ascorbate + O2

peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O

Homo sapiens

P19021

-

745459

-

-

?

peptidylglycine + ascorbate + O2

peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O

Homo sapiens

-

amidation of neurohormonal peptides

438581

-

438581

?

peptidylglycine + ascorbate + O2

peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O

Homo sapiens

P19021

most mammalian bioactive peptides possess a C-terminal amino acid amide moiety. Amidated peptides are produced in vivo by the enzymatic cleavage of a precursor with a C-terminal glycine residue. The enzyme catalyzes the key step in the oxidation of the glycine-extended precursors to the alpha-amidated peptide

720977

-

-

?

peptidylglycine + ascorbate + O2

peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O

Mus musculus

-

-

728178

-

-

?

peptidylglycine + ascorbate + O2

peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O

Mus musculus

-

peptidylglycine alpha-hydroxylating monooxygenase reaction

657678

the carbinol is the substrate for the peptidylamidoglycolate lyase reaction, EC 4.3.2.5, forming glyoxylate and amidated peptide

-

?

peptidylglycine + ascorbate + O2

peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O

Ovis aries

-

-

438565

-

438565

?

peptidylglycine + ascorbate + O2

peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O

Rattus norvegicus

-

-

438562

-

438562

?

peptidylglycine + ascorbate + O2

peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O

Rattus norvegicus

-

-

438566

-

438566

?

peptidylglycine + ascorbate + O2

peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O

Rattus norvegicus

-

-

438571

-

438571

?

peptidylglycine + ascorbate + O2

peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O

Rattus norvegicus

-

-

438572

-

438572

?

peptidylglycine + ascorbate + O2

peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O

Rattus norvegicus

-

-

438574

-

438574

?

peptidylglycine + ascorbate + O2

peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O

Rattus norvegicus

-

-

438587

-

438587

?

peptidylglycine + ascorbate + O2

peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O

Rattus norvegicus

-

-

438588

-

438588

?

peptidylglycine + ascorbate + O2

peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O

Rattus norvegicus

-

-

438589

-

438589

?

peptidylglycine + ascorbate + O2

peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O

Rattus norvegicus

-

-

438593

-

438593

?

peptidylglycine + ascorbate + O2

peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O

Rattus norvegicus

-

-

438594

-

438594

?

peptidylglycine + ascorbate + O2

peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O

Rattus norvegicus

-

-

658004

-

-

?

peptidylglycine + ascorbate + O2

peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O

Rattus norvegicus

P14925

-

438591

-

438591

?

peptidylglycine + ascorbate + O2

peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O

Rattus norvegicus

P14925

-

698481, 744349, 744361, 745298

-

-

?

peptidylglycine + ascorbate + O2

peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O

Rattus norvegicus

-

peptidylglycine alpha-hydroxylating monooxygenase reaction

657678, 658458

the carbinol is the substrate for the peptidylamidoglycolate lyase reaction, EC 4.3.2.5, forming glyoxylate and amidated peptide

-

?

peptidylglycine + ascorbate + O2

peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O

Rattus norvegicus

P14925

the enzyme catalyzes the final reaction in the maturation of alpha-amidated peptide hormones

745459

-

-

?

peptidylglycine + ascorbate + O2

peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O

Sus scrofa

-

-

438563

-

438563

?

peptidylglycine + ascorbate + O2

peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O

Sus scrofa

-

-

438564

-

438564

?

peptidylglycine + ascorbate + O2

peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O

Sus scrofa

-

-

438577

-

438577

?

peptidylglycine + ascorbate + O2

peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O

Sus scrofa

-

-

438579

-

438579

?

peptidylglycine + ascorbate + O2

peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O

Xenopus laevis

-

-

438569

-

438569

?

peptidylglycine + ascorbate + O2

peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O

Xenopus laevis

-

-

438573

-

438573

?

peptidylglycine + ascorbate + O2

peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O

Xenopus laevis

-

-

438576

-

438576

?

peptidylglycine + ascorbate + O2

peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O

Xenopus laevis

-

-

438583

-

438583

?

peptidylglycine + ascorbate + O2

peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O

Xenopus laevis

-

-

438584

-

438584

?

peptidylglycine + ascorbate + O2

peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O

Xenopus laevis

-

-

438585

-

438585

?

peptidylglycine + ascorbate + O2

peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O

Xenopus laevis

-

-

438590

-

438590

?

proopiomelanocortin peptide + ascorbate + O2

proopiomelanocortin peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O

Homo sapiens

P19021

a POMC 18-kDa fragment

745327

-

-

?

proopiomelanocortin peptide + ascorbate + O2

proopiomelanocortin peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O

Rattus norvegicus

P14925

a POMC 18-kDa fragment

745327

-

-

?

additional information

?

-

Drosophila melanogaster

O01404

C-terminally amidation of a range of peptides by the copper-dependent enzyme, peptidylglycine alpha-amidating monooxygenase, PAM. PAM-dependent amidation of POMC peptides in AtT20 cells

745332

-

-

?

additional information

?

-

Dugesia japonica

Q4W7B5

enzyme is required for amidation of the C-terminus of neuropeptides

658685

-

-

?

additional information

?

-

Homo sapiens

-

enzyme is required for amidation of autocrine growth factors causing proliferation in tumor cells

659916

-

-

?

additional information

?

-

Homo sapiens

-

PHM replaces the pro-(S) hydrogen of the carboxy-terminal glycine with a hydroxyl group to form an alpha-hydroxyglycine intermediate

699036

-

-

?

additional information

?

-

Homo sapiens

P19021

C-terminally amidation of a range of peptides by the copper-dependent enzyme, peptidylglycine alpha-amidating monooxygenase, PAM

745332

-

-

?

additional information

?

-

Homo sapiens

P19021

constitutive-like secretion of POMC products in recombinant enzyme-expressing cells

745327

-

-

?

additional information

?

-

Mus musculus

-

enzyme is involved in peptide posttranslational activation

657678

-

-

?

additional information

?

-

Mus musculus

-

PAM is the only enzyme capable of amidating peptides, amidated peptides are numerous

697630

-

-

?

additional information

?

-

Mus musculus

P97467

C-terminally amidation of a range of peptides by the copper-dependent enzyme, peptidylglycine alpha-amidating monooxygenase, PAM

745332

-

-

?

additional information

?

-

Rattus norvegicus

-

cleavage of CN bond in N-acetylated glycines, R-CO-NH-CH2-COOH, e.g. found in neuropeptide prohormones, inhibition of the enzyme leads to a decrease in alpha-aminated peptide production and accumulation of glycine-extended precursors

657649

-

-

?

additional information

?

-

Rattus norvegicus

-

enzyme catalyzes the biosynthesis of peptide hormones through radical cleavage of the C-terminal glycine residues of the corresponding prohormones

658977

-

-

?

additional information

?

-

Rattus norvegicus

-

enzyme catalyzes the production of neurohormones and neurotransmitters

658976

-

-

?

additional information

?

-

Rattus norvegicus

-

enzyme is involved in peptide posttranslational activation

657678

-

-

?

additional information

?

-

Rattus norvegicus

-

enzyme is required for amidation of autocrine growth factors causing proliferation in tumor cells

659847

-

-

?

additional information

?

-

Rattus norvegicus

-

enzyme might be involved in secretory vesicle budding and fusion in the cardiac ANP-secretory pathway

658458

-

-

?

additional information

?

-

Rattus norvegicus

-

PAM is the rate-limiting enzyme for the generation of carboxy-terminal alpha-amidated neuropeptides

698532

-

-

?

additional information

?

-

Rattus norvegicus

P14925

constitutive-like secretion of POMC products in recombinant enzyme-expressing cells

745327

-

-

?

additional information

?

-

Xenopus laevis

-

peptide alpha-amidation, enzyme is responsible for formation of peptide C-terminal amide

660417

-

-

?