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1.14.16.1: phenylalanine 4-monooxygenase

This is an abbreviated version!
For detailed information about phenylalanine 4-monooxygenase, go to the full flat file.

Word Map on EC 1.14.16.1

Reaction

L-phenylalanine
+
a 5,6,7,8-tetrahydropteridine
 +
O2
=
L-tyrosine
 +
a 4a-hydroxy-5,6,7,8-tetrahydropteridine

Synonyms

cePAH, DicPAH, EC 1.14.3.1, EC 1.99.1.2, HPAH, L-phenylalanine 4-hydroxylase, oxygenase, phenylalanine 4-mono-, P4H, PAH, PheH, phenylalaninase, phenylalanine 4-hydroxylase, phenylalanine hydroxylase, phenylalanine monooxygenase, PheOH, phhA

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.16 With reduced pteridine as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.16.1 phenylalanine 4-monooxygenase

Specific Activity

Specific Activity on EC 1.14.16.1 - phenylalanine 4-monooxygenase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.00009
-
activity in liver of males living in an area with high emissions of SO2 and nitrogen oxides, cofactor tetrahydrobiopterin
0.00033
-
activity in liver of males, cofactor tetrahydrobiopterin
0.0009
mutant enzyme V388M, using S-carboxymethyl-L-cysteine as substrate
0.00097
-
activity in liver of males, cofactor tetrahydrobiopterin
0.0014
0.0015
mutant enzyme Y414C, using S-carboxymethyl-L-cysteine as substrate
0.0016
mutant enzyme R261Q, using S-carboxymethyl-L-cysteine as substrate
0.0049
L-phenylalanine-activated mutant enzyme S231F, at 25°C
0.0052
non-L-phenylalanine-activated mutant enzyme S231F, at 25°C
0.006
-
activity in liver of males, cofactor 6,7-dimethyltetrahydropterin
0.055
-
I65T mutant enzyme, cofactor 6-methyltetrahydropterin
0.073
wild type enzyme, using S-carboxymethyl-L-cysteine as substrate
0.09
-
cofactor 6,7-dimethyltetrahydropterin
0.099
R270K mutant enzyme, expression in the absence of glycerol in the growth medium, cofactor 6-methyltetrahydropterin
0.105
-
activity in liver of males living in an area with high emissions of SO2 and nitrogen oxides, cofactor 6,7-dimethyltetrahydropterin
0.106
-
-
0.17
-
cofactor 6-methyltetrahydropterin
0.225
-
liver enzyme
0.247
-
activity in liver of males, cofactor 6,7-dimethyltetrahydropterin
0.307
mutant enzyme W180F, at 30°C, using L-phenylalanine as substrate
0.342
mutant enzyme W180F, at 30°C, using 5,6,7,8-tetrahydrobiopterin as substrate
0.347
wild type enzyme, at 30°C, using 5,6,7,8-tetrahydrobiopterin as substrate
0.408
-
V388M mutant enzyme, cofactor tetrahydrobiopterin
0.4169
non-L-phenylalanine-activated wild type enzyme, at 25°C
0.424
-
maltose-binding-protein phenylalanine hydroxylase fusion protein, dimeric form
0.44
-
liver enzyme
0.505
mutant enzyme V388M, using L-phenylalanine as substrate
0.536
-
R261Q mutant enzyme, cofactor tetrahydrobiopterin
0.69
wild type enzyme, at 30°C, using L-tryptophan as substrate
0.7418
L-phenylalanine-activated wild type enzyme, at 25°C
0.745
-
V388M mutant enzyme, cofactor 6-methyltetrahydropterin
0.75
mutant enzyme R408W, using L-phenylalanine as substrate, in 100 mM Na-HEPES buffer, pH 7.0, at 25°C
0.77
-
mutant R68V, preincubation with L-phenylalanine
0.78
-
R261Q mutant enzyme, cofactor 6-methyltetrahydropterin
0.893
mutant enzyme W180F, at 30°C, using L-tryptophan as substrate
1.05
mutant enzyme L101Y/W180F, at 30°C, using 5,6,7,8-tetrahydrobiopterin as substrate
1.08
-
mutant C237R
1.1
-
wild-type, preincubation with L-phenylalanine
1.13
-
mutant C237R, preincubation with L-phenylalanine
1.2
mutant enzyme Y414C, using L-phenylalanine as substrate
1.283
-
maltose-binding-protein phenylalanine hydroxylase fusion protein, tetrameric form
1.32
mutant enzyme R155H, using L-phenylalanine as substrate, in 100 mM Na-HEPES buffer, pH 7.0, at 25°C
1.49
mutant enzyme R261Q, using L-phenylalanine as substrate
1.6
-
fetal liver enzyme
1.64
mutant enzyme D143G, using L-phenylalanine as substrate, in 100 mM Na-HEPES buffer, pH 7.0, at 25°C
1.7
-
pH 7.0, 25°C, wild-type, dimer
1.725
mutant enzyme R68S, using L-phenylalanine as substrate
1.742
-
recombinant wild-type enzyme, cofactor tetrahydrobiopterin
1.76
-
adult liver enzyme
1.77
mutant enzyme L101Y, at 30°C, using L-tryptophan as substrate
1.773
V388M mutant enzyme, expression in the absence of glycerol in the growth medium, cofactor 6-methyltetrahydropterin
1.8
-
enzyme form I
1.9
wild type enzyme, using L-phenylalanine as substrate
1.98
substrate L-phenylalanine, mutant N223D
10.16
mutant enzyme L101Y, at 30°C, using L-phenylalanine as substrate
10.2
-
in 100 mM Na-HEPES, pH 7.0 at 37°C
1150
-
mutant Y325F, 25°C, pH 7.0
12.5 - 13
-
-
1230
-
wild-type, 25°C, pH 7.0
1310
-
mutant Y325L, 25°C, pH 7.0
14
-
truncated enzyme containing C-terminal 334 amino acids
1500
-
mutant Y325L, preincubation with L-phenylalanine, 25°C, pH 7.0
2.1
-
micromol L-Tyr/min/mg, wild-type, pH 7.0, 25°C, without L-Phe preincubated enzyme
2.14
-
mutant R68V
2.2
-
mutant R68A, preincubation with L-phenylalanine
2.25
mutant enzyme I65T, using L-phenylalanine as substrate
2.34
substrate tetrahydrobiopterin, mutant N223D
2.49
-
recombinant wild-type enzyme, cofactor 6-methyltetrahydropterin
2.76
mutant enzyme L348V, using L-phenylalanine as substrate, in 100 mM Na-HEPES buffer, pH 7.0, at 25°C
2.84
substrate tetrahydrobiopterin, mutant T427P
2.91
V388M mutant enzyme, expression in the presence of glycerol in the growth medium, cofactor 6-methyltetrahydropterin
2.94
-
mutant R68A
2.97
-
mutant C237A
2.98
wild type enzyme, using L-phenylalanine as substrate, in 100 mM Na-HEPES buffer, pH 7.0, at 25°C
290
-
mutant Y325A, 25°C, pH 7.0
3.17
substrate L-phenylalanine, mutant T427P
3.25
-
wild-type
3.32
mutant enzyme P416Q, using L-phenylalanine as substrate, in 100 mM Na-HEPES buffer, pH 7.0, at 25°C
3.37
wild type enzyme, at 30°C, using L-phenylalanine as substrate
3.48
substrate L-phenylalanine, wild-type
3.62
mutant enzyme L101Y/W180F, at 30°C, using L-tryptophan as substrate
3.67
substrate L-phenylalanine, mutant N426D
3.74
substrate L-phenylalanine, mutant N32D
3.81
-
mutant C237D
300
-
mutant Y325A, preincubation with L-phenylalanine, 25°C, pH 7.0
3630
-
mutant Y325F, preincubation with L-phenylalanine, 25°C, pH 7.0
3640
-
wild-type, preincubation with L-phenylalanine, 25°C, pH 7.0
4.19
-
mutant C237D, preincubation with L-phenylalanine
4.37
substrate L-phenylalanine, mutant G33A
4.39
substrate tetrahydrobiopterin, mutant N426D
4.95
-
pH 7.0, 25°C, wild-type, tetramer
5
-
phosphorylated recombinant wild-type enzyme, S16N and S16D mutant enzyme
5.1
-
S16A and S16K mutant enzymes
5.16
substrate L-phenylalanine, mutant G33V
5.2
-
S16E and S16Q mutant enzyme
5.32
substrate tetrahydrobiopterin, wild-type
6.19
substrate L-phenylalanine, mutant K113P
6.41
substrate tetrahydrobiopterin, mutant G33V
6.48
-
pH 7.0, 25°C, N-terminal deletion mutant
6.5
-
micromol L-Tyr/min/mg, mutant Q215K/N216Y, pH 7.0, 25°C, with L-Phe preincubated enzyme
6.8
-
recombinant enzyme
8.32
-
pH 7.0, 25°C, N-terminal plus C-terminal deletion mutant
8.56
substrate tetrahydrobiopterin, mutant K113P
additional information