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1.14.15.4: steroid 11beta-monooxygenase

This is an abbreviated version!
For detailed information about steroid 11beta-monooxygenase, go to the full flat file.

Word Map on EC 1.14.15.4

Reaction

A steroid
+ 2 reduced adrenodoxin +
O2
 + 2 H+ =
an 11beta-hydroxysteroid
 + 2 oxidized adrenodoxin +
H2O

Synonyms

11-hydroxylase, 11 beta-hydroxylase, 11-beta hydroxylase, 11-beta-hydroxylase, 11-hydroxylase, 11beta hydroxylase, 11beta-hydroxylase, ALDOS, Aldosterone synthase, Aldosterone-synthesizing enzyme, C450XIB2, CYP11B1, CYP11B2, cyp11beta, CYPXIB, CYPXIB1, CYPXIB2, CYPXIB3, cytochrome 11beta-hydroxylase, cytochrome P-45011-beta, cytochrome P450 11B1, cytochrome P450 11B2, cytochrome P450 11beta-hydroxylase, cytochrome P45011beta, cytochrome P45011beta-hydroxylase, EC 1.14.1.6, EC 1.99.1.7, P-450(11 beta,aldo), P-450Aldo, P-450c11, P-450C18, P-450XIB1, P450 11beta-hydroxylase, P450(11 beta)-DS, P45011beta, P450C11, Steroid 11-beta-hydroxylase, steroid 11-hydroxylase, steroid 11beta-hydroxylase, steroid 11beta-monooxygenase, steroid 11beta/18-hydroxylase, Steroid 18-hydroxylase, steroid-11beta-hydroxylase

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.15 With reduced iron-sulfur protein as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.15.4 steroid 11beta-monooxygenase

Cloned

Cloned on EC 1.14.15.4 - steroid 11beta-monooxygenase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
2 P-45011beta cDNAs, pc P-450 11beta-3 and -2, cloned into and expressed in COS-7 cells
-
cDNA transfected into COS-7 cells simultaneously with bovine adrenodoxin cDNA expression plasmid
-
CYP11B1 genotyping, overview. Expression of wild-type and mutant CYP11B1s in COS-7 cells
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CYP11B3 enzyme cDNA cloned into and expressed in COS-7 cells
-
DNA and amino acid sequence analysis, expression analysis and regulation
-
enzyme expression analysis, expression of CYP11B1 and CYP11B2 in H295R adrenocortical cells
-
expressed in Escherichia coli
-
expression analysis
-
expression in Escherichia coli
expression in Escherichia coli, coexpression with molecular chaperones GroES and GroEL
-
expression of C-terminally His6-tagged CYP11B2 mature form without the mitochondrial targeting sequence of 24 residues at the N-terminus in Escherichia coli, co-expression with the molecular chaperones GroEL and GroES
expression of CYP11B1 and CYP11B2 in V79 cells
-
expression of CYP11B2 in Schizosaccharomyces pombe and in V79 MZh cells
-
expression of mature form of CYP11B1 protein without the mitochondrial targeting peptide and with N-terminal tag MAKKTSS added to the 26th residue of CYP11B1, and with C-terminal His6-tag, coexpression with GroEL/ES in Escherichia coli strain JM109
expression of mature form of CYP11B2 protein without the mitochondrial targeting peptide and with N-terminal tag MAKKTSS added to the 30th residue of CYP11B2, and with C-terminal His6-tag, coexpression with GroEL/ES in Escherichia coli strain JM109
expression of the different enzyme-DHFR fusion constructs in NCI-H295 cells
-
expression of wild-type CYP11B1 and of chimeric mutant CYP11B1/B2 in HEK-293 cells
-
full-length P45011B1 cDNA cloned into and expressed in lung fibroblast derived V79 Chinese hamster cells
-
gene CYP11B, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic tree, expression analysis
-
gene cyp11b, sequence comparison and expression analysis
gene CYP11B1 variants, DNA and amino acid sequence analysis of 512 samples from hypertensive patients, genotyping, polymorphism frequency at positions 1889 and 1859, overview
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gene CYP11B1, DNA and aamino acid sequence determination of wild-type and mutant genes
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gene CYP11B1, DNA and amino acid sequence determination and analysis, genotyping
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gene CYP11B1, DNA and amino acid sequence determination and analysis, localization on chromosome 8 adjacent to gene CYP11B2, genotyping and determination of polymorphisms in 1428 samples of Caucasian families, overview
-
gene CYP11B1, DNA and amino acid sequence determination and analysis, localization on chromosome 8 adjacent to gene CYP11B2, genotyping of CwtCG and TconvGTA haplotypes
-
gene CYP11B1, DNA and amino acid sequence determination, located on chromosome 8q22
-
gene CYP11B1, located on chromosome 8q22, DNA and amino acid sequence determination of wild-type and mutant enzymes from different samples, expression in COS-7 cells
-
gene CYP11B1, maps to chromosome 8q24, DNA and amino acid sequence determination and analysis of wild-type and mutant enzymes, genotyping, the breakpoint in 3p might be a candidate region affecting variations in pheotypes of enzyme deficiency, overview
-
gene CYP11B2, located on chromosome 8q22, DAN and amino acid sequence determination, determination and analysis of the -344 polymorphism
-
genes CYP11B1 and CYP11B2, DNA and amino acid sequence determination and analysis, expression of wild-type and mutant enzymes in COS-7 cells
overexpressed in V79-4 Chinese hamster lung cell line
P-450(11beta,aldo) expressed in COS-7 cells
-
stable expression of human CYP11B2 or CYP11B1 enzymes in V79 Chinese hamster cells
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two alternative splicing isoforms P450(11beta)-1 and P450(11beta)-2 cDNAs are cloned
-