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1.14.15.16: vitamin D3 24-hydroxylase

This is an abbreviated version!
For detailed information about vitamin D3 24-hydroxylase, go to the full flat file.

Word Map on EC 1.14.15.16

Reaction

calcidiol
+ 2 reduced adrenodoxin + 2 H+ +
O2
=
secalciferol
+ 2 oxidized adrenodoxin +
H2O

Synonyms

1,25-(OH)2D3-24-hydroxylase, 1,25-dihydroxyvitamin D3 24-hydroxylase, 1alpha,25-dihydroxyvitamin D3 24-hydroxylase, 24-hydroxylase, 24-OHase, 24OHase, 25-hydroxyvitamin D-24-hydroxylase, 25-hydroxyvitamin D3-24-hydroxylase, 25-OH-D3-24-hydroxylase, CYP24A1, cytochrome P450 24A1, EC 1.14.13.126, P450cc24, vitamin D 24-hydroxylase, vitamin D-24-hydroxylase

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.15 With reduced iron-sulfur protein as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.15.16 vitamin D3 24-hydroxylase

Purification

Purification on EC 1.14.15.16 - vitamin D3 24-hydroxylase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant active His-tagged enzyme from Escherichia coli membranes by nickel affinity and hydrophobic interaction chromatography and dialysis, adrenodoxin-affinity chromatography is not successful
recombinant N-terminally His6-tagged wild-type and mutant enzymes from Escherichia coli strain DH5alpha F'IQ by nickel affinity chromatography and gel filtration, copurification with added recombinant bovine adrenodoxin for enzyme-cofctor complex preparation
-
recombinant N-terminally maltose-binding-protein fused enzyme from Escherichia coli by affinity chromatography to homogeneity
Talon metal affinity column chromatography
-