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1.14.15.16: vitamin D3 24-hydroxylase

This is an abbreviated version!
For detailed information about vitamin D3 24-hydroxylase, go to the full flat file.

Word Map on EC 1.14.15.16

Reaction

calcidiol
+ 2 reduced adrenodoxin + 2 H+ +
O2
=
secalciferol
 + 2 oxidized adrenodoxin +
H2O

Synonyms

1,25-(OH)2D3-24-hydroxylase, 1,25-dihydroxyvitamin D3 24-hydroxylase, 1alpha,25-dihydroxyvitamin D3 24-hydroxylase, 24-hydroxylase, 24-OHase, 24OHase, 25-hydroxyvitamin D-24-hydroxylase, 25-hydroxyvitamin D3-24-hydroxylase, 25-OH-D3-24-hydroxylase, CYP24A1, cytochrome P450 24A1, EC 1.14.13.126, P450cc24, vitamin D 24-hydroxylase, vitamin D-24-hydroxylase

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.15 With reduced iron-sulfur protein as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.15.16 vitamin D3 24-hydroxylase

Expression

Expression on EC 1.14.15.16 - vitamin D3 24-hydroxylase

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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
CYP24A1 is transcriptionally induced in vitamin D target cells by the action of 1alpha,25-dihydroxyvitamin D3
enzyme expression is upregulated in cancer cells
-
intraperitoneal injection of 1,25-dihydroxyvitamin D3 induces dose- and time-dependent increases in both 24-OHase mRNA abundance and enzyme activity in mouse kidney reaching a maximum between 4-8 h, after which it decreases and then maintains a steady value between 18-72 h. Similarly, 1,25-dihydroxyvitamin D3-induced increases in both 24-OHase mRNA and activity are apparent in the duodenum. Although 1,25-dihydroxyvitamin D3 markedly the amount of 24-OHase mRNA in skin between 4 and 6 h, enzyme activity is not detected in this tissue. Pretreatment of mice with cycloheximide (0.4mg/g) potentiates the increase in 24-OHase mRNA abundance but blocks the increase in 24-OHase activity, induced by 1,25-dihydroxyvitamin D3 in kidney and duodenum
-
methyltransferase inhibitor 5-aza-2'-deoxycytidine treatment results in an over 50fold induction of CYP24A1 mRNA expression in Coga1A and HT-29 cells. In colon cancer cells, CYP24A1 gene expression is inducible by methyltransferase and some histone deacetylase inhibitors in a cell line-dependent manner
-
replacement of calcitriol (3 ng per day) significantly increases 24-hydroxylase activity by 17% in rats with renal failure, although the activity remains 15% lower than the controls
-
the enzymatic activity of intestinal 24-hydroxylase is significantly lower in renal failure rats compared to control sham operated rats
-
the enzyme is upregulated in tumor cells compared with normal tissue
-
the vitamin D receptor bound to 1alpha,25(OH)2D3 induces expression of mitochondrial cytochrome P450 24A1