1.14.14.9: 4-hydroxyphenylacetate 3-monooxygenase

This is an abbreviated version!
For detailed information about 4-hydroxyphenylacetate 3-monooxygenase, go to the full flat file.

Word Map on EC 1.14.14.9

1.14.14.9

flavin

3,4-dihydroxyphenylacetate

baumannii

flavin-dependent

tyrosol

hydroxytyrosol

fmnh

two-protein

piceatannol

synthesis

Reaction

Synonyms

4 HPA 3-hydroxyylase, 4-HPA hydroxylase, 4-hydroxyphenylacetate 3-hydroxylase, 4-hydroxyphenylacetic acid 3-hydroxylase, 4HPA 3-monooxygenase, 4HPA3H, C2-hpah, EC 1.14.13.3, HPA 3-hydroxylase, HpaB, hpaBC, HpaC, HPAH, More, p-hydroxyphenylacetate 3-hydroxylase, p-hydroxyphenylacetate hydroxylase, p-hydroxyphenylacetic 3-hydroxylase, TPY_2462, two-component p-hydroxyphenylacetate hydroxylase

ECTree

1 Oxidoreductases

1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen

1.14.14 With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor

1.14.14.9 4-hydroxyphenylacetate 3-monooxygenase

Advanced search results

Do not include text mining results

Include

results (more...)

Include

results (more...)

Results	in table
4	Activating Compound
1749	AA Sequence
7	Application
1	CAS Registry Number
20	Cloned(Commentary)
72	Cofactor
8	Crystallization (Commentary)
2	General Information
3	General Stability
21	Inhibitors
31	kcat/KM [mM/s]
57	KM Value [mM]
2	Localization
5	Metals/Ions
24	Molecular Weight [Da]
49	Natural Substrates/ Products (Substrates)
367	Organism
7	Pathway
12	PDB ID
21	pH Optimum
4	pH Range
49	Protein Variants
17	Purification (Commentary)
20	Reaction
4	Reaction Type
71	Reference
1	Source Tissue
20	Specific Activity [micromol/min/mg]
4	Storage Stability
187	Substrates and Products (Substrate)
28	Subunits
70	Synonyms
1	Systematic Name
16	Temperature Optimum [°C]
1	Temperature Stability [°C]
34	Turnover Number [1/s]

Purification

print visible entries

print all entries

Go back to the abbreviated version
of the Enzyme Summary Page.

Purification on EC 1.14.14.9 - 4-hydroxyphenylacetate 3-monooxygenase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.

top print hide

Go to Purification (commentary) Search

2 entries

affinity chromatography, gel filtration

Escherichia coli

438893

ammonium sulfate precipitation, DEAE-Sepharose column chromatography, phenyl Sepharose column chromatography, and G-25 gel filtration

Acinetobacter baumannii

726623

ammonium sulfate precipitation, gel filtration, affinity chromatography

Pseudomonas putida

438887

ammonium sulfate precipitation, ion exchange

Escherichia coli

438896

ammonium sulfate precipitation, ion-exchange, gel filtration

Pseudomonas putida

438895

ammonium sulfate precipitation, ion-exchange, gel filtration. Affinity chromatography for the expressed cholin-binding domain containing HpaB protein

Escherichia coli

438899

difficult because of the low stability of the enzyme in solution

Escherichia coli

438894

from membranes by solubilization with 0.1% Triton X-100, anion exchange chromatography, dialysis, exchange of detergent to dodecyl-beta-D-maltoside, another ion exchange chromatography, and gel filtration, further purification of HpaB by repetitive crystallization, method overview

Thermus thermophilus

Q5SJP8

710753

partially

Klebsiella pneumoniae

438898

protamine sulfate and ammonium sulfate

Pseudomonas putida

438886

protamine sulfate precipitation, ion-exchange, gel filtration

Acinetobacter baumannii

438900

recombinant enzyme components C1 and C2 from Escherichia coli strain BL21(DE3), C1 4.0fold, C2 1.81fold

Acinetobacter baumannii

Q6Q271, Q6Q272

658203

recombinant enzyme from Escherichia coli strain DH5alpha by affinity chromatography on a 4-hydroxyphenylacetate-coupled amino-agarose column to near homogeneity

Geobacillus sp.

Q4L1M7

686265

ultracentrifugation, ammonium sulfate precipitation

Arthrobacter sp.

288040