1.14.14.9: 4-hydroxyphenylacetate 3-monooxygenase

This is an abbreviated version!
For detailed information about 4-hydroxyphenylacetate 3-monooxygenase, go to the full flat file.

Reaction

Synonyms

4 HPA 3-hydroxyylase, 4-HPA hydroxylase, 4-hydroxyphenylacetate 3-hydroxylase, 4-hydroxyphenylacetic acid 3-hydroxylase, 4HPA 3-monooxygenase, 4HPA3H, C2-hpah, EC 1.14.13.3, HPA 3-hydroxylase, HpaB, hpaBC, HpaC, HPAH, More, p-hydroxyphenylacetate 3-hydroxylase, p-hydroxyphenylacetate hydroxylase, p-hydroxyphenylacetic 3-hydroxylase, TPY_2462, two-component p-hydroxyphenylacetate hydroxylase

ECTree

     1 Oxidoreductases

         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen

             1.14.14 With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor

                1.14.14.9 4-hydroxyphenylacetate 3-monooxygenase

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Results	in table
4	Activating Compound
1749	AA Sequence
7	Application
1	CAS Registry Number
20	Cloned(Commentary)
72	Cofactor
8	Crystallization (Commentary)
2	General Information
3	General Stability
21	Inhibitors
31	kcat/KM [mM/s]
57	KM Value [mM]
2	Localization
5	Metals/Ions
24	Molecular Weight [Da]
49	Natural Substrates/ Products (Substrates)
367	Organism
7	Pathway
12	PDB ID
21	pH Optimum
4	pH Range
49	Protein Variants
17	Purification (Commentary)
20	Reaction
4	Reaction Type
71	Reference
1	Source Tissue
20	Specific Activity [micromol/min/mg]
4	Storage Stability
187	Substrates and Products (Substrate)
28	Subunits
70	Synonyms
1	Systematic Name
16	Temperature Optimum [°C]
1	Temperature Stability [°C]
34	Turnover Number [1/s]

KM Value

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KM Value on EC 1.14.14.9 - 4-hydroxyphenylacetate 3-monooxygenase

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KM VALUE [mM]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

0.0461

(3,4-dihydroxyphenyl)acetate

Escherichia coli

-

pH 7.5, 23°C

764477

0.0339

2-(4-hydroxyphenyl)ethanol

Escherichia coli

-

pH 7.5, 23°C

764477

-

0.0094

3-hydroxyphenylacetate

Escherichia coli

-

pH 7.5, 23°C

764477

0.054

4-coumarate

Escherichia coli

-

pH 7.5, 23°C

764477

0.132 - 0.388

4-coumaric acid

6 entries

0.014 - 26

4-hydroxyphenylacetate

8 entries

0.0247

DL-4-hydroxymandelic acid

Escherichia coli

-

pH 7.5, 23°C

764477

0.0031 - 0.0042

FAD

2 entries

0.0021

FMN

Escherichia coli

-

-

438899

0.515

methyl 4-hydroxybenzoate

Escherichia coli

-

pH 7.5, 23°C

764477

0.012 - 28

NADH

8 entries

0.192 - 1.062

naringenin

6 entries

0.253

phenol

Escherichia coli

-

pH 7.5, 23°C

764477

0.16

phenylacetic acid

Pseudomonas putida

-

-

438895

0.144 - 0.442

resveratrol

6 entries

0.0026

riboflavin

Escherichia coli

-

-

438899

0.177 - 0.491

umbelliferone

6 entries

additional information

additional information

6 entries

-

0.132

4-coumaric acid

Escherichia coli

A0A140NG21

mutant XS6, pH 7, 30°C

765827

0.138

4-coumaric acid

Escherichia coli

A0A140NG21

wild-type, pH 7, 30°C

765827

0.211

4-coumaric acid

Escherichia coli

A0A140NG21

mutant XS4, pH 7, 30°C

765827

0.235

4-coumaric acid

Escherichia coli

A0A140NG21

mutant XS5, pH 7, 30°C

765827

0.236

4-coumaric acid

Escherichia coli

A0A140NG21

mutant XS3, pH 7, 30°C

765827

0.388

4-coumaric acid

Escherichia coli

A0A140NG21

mutant XS2, pH 7, 30°C

765827

0.014

4-hydroxyphenylacetate

Acinetobacter baumannii

-

apparent, with FAD

438900

0.019

4-hydroxyphenylacetate

Acinetobacter baumannii

-

apparent, with FMN

438900

0.038

4-hydroxyphenylacetate

Pseudomonas putida

-

-

438895

0.2

4-hydroxyphenylacetate

Pseudomonas putida

-

-

438887

15

4-hydroxyphenylacetate

Acinetobacter baumannii

Q6Q271, Q6Q272

native C1-C2 system or complete enzyme, respectively, pH 7.5, with FAD as cosubstrate

658203

16

4-hydroxyphenylacetate

Acinetobacter baumannii

Q6Q271, Q6Q272

recombinant C1-C2 system or complete enzyme, respectively, pH 7.5, with FAD as cosubstrate

658203

25

4-hydroxyphenylacetate

Acinetobacter baumannii

Q6Q271, Q6Q272

native C1-C2 system or complete enzyme, respectively, pH 7.5, with FMN as cosubstrate

658203

26

4-hydroxyphenylacetate

Acinetobacter baumannii

Q6Q271, Q6Q272

recombinant C1-C2 system or complete enzyme, respectively, pH 7.5, with FMN as cosubstrate

658203

0.0031

FAD

Escherichia coli

-

-

438899

0.0042

FAD

Pseudomonas putida

-

-

438895

0.012

NADH

Acinetobacter baumannii

-

apparent, with FMN

438900

0.028

NADH

Acinetobacter baumannii

-

apparent, with FAD

438900

0.04

NADH

Escherichia coli

-

-

438899

0.041

NADH

Pseudomonas putida

-

-

438895

0.059

NADH

Pseudomonas putida

-

-

438887

21

NADH

Acinetobacter baumannii

Q6Q271, Q6Q272

native C1-C2 system or complete enzyme, respectively, pH 7.5, with FMN as cosubstrate

658203

22

NADH

Acinetobacter baumannii

Q6Q271, Q6Q272

native and recombinant C1-C2 system or complete enzyme, respectively, pH 7.5, with FAD as cosubstrate

658203

28

NADH

Acinetobacter baumannii

Q6Q271, Q6Q272

recombinant C1-C2 system or complete enzyme, respectively, pH 7.5, with FMN as cosubstrate

658203

0.192

naringenin

Escherichia coli

A0A140NG21

mutant XS6, pH 7, 30°C

765827

0.35

naringenin

Escherichia coli

A0A140NG21

wild-type, pH 7, 30°C

765827

0.417

naringenin

Escherichia coli

A0A140NG21

mutant XS3, pH 7, 30°C

765827

0.628

naringenin

Escherichia coli

A0A140NG21

mutant XS4, pH 7, 30°C

765827

0.661

naringenin

Escherichia coli

A0A140NG21

mutant XS5, pH 7, 30°C

765827

1.062

naringenin

Escherichia coli

A0A140NG21

mutant XS2, pH 7, 30°C

765827

0.144

resveratrol

Escherichia coli

A0A140NG21

mutant XS6, pH 7, 30°C

765827

0.174

resveratrol

Escherichia coli

A0A140NG21

wild-type, pH 7, 30°C

765827

0.236

resveratrol

Escherichia coli

A0A140NG21

mutant XS3, pH 7, 30°C

765827

0.32

resveratrol

Escherichia coli

A0A140NG21

mutant XS5, pH 7, 30°C

765827

0.405

resveratrol

Escherichia coli

A0A140NG21

mutant XS2, pH 7, 30°C

765827

0.442

resveratrol

Escherichia coli

A0A140NG21

mutant XS4, pH 7, 30°C

765827

0.177

umbelliferone

Escherichia coli

A0A140NG21

mutant XS6, pH 7, 30°C

765827

0.205

umbelliferone

Escherichia coli

A0A140NG21

mutant XS4, pH 7, 30°C

765827

0.217

umbelliferone

Escherichia coli

A0A140NG21

wild-type, pH 7, 30°C

765827

0.266

umbelliferone

Escherichia coli

A0A140NG21

mutant XS3, pH 7, 30°C

765827

0.346

umbelliferone

Escherichia coli

A0A140NG21

mutant XS5, pH 7, 30°C

765827

0.491

umbelliferone

Escherichia coli

A0A140NG21

mutant XS2, pH 7, 30°C

765827

additional information

additional information

Escherichia coli

-

kinetics, FADH2/FAD binding capacity, enzyme kinetics in a coupled assay with HpaC, a NAD(P)H-flavin oxidoreductase

658006

-

additional information

additional information

Acinetobacter baumannii

-

kinetics and thermodynamics, determination of redox potentials of FMN reductase component C1-bound FMN in presence or absence of 4-hydroxyphenylacetate, overview

671960

-

additional information

additional information

Acinetobacter baumannii

-

transient kinetic study, overview

674577

-

additional information

additional information

Acinetobacter baumannii

-

steady-steady kinetics, kinetics of FMN reduction, transfer from C1 to C2 component, and FMNH- release, thermodynamic data, including redox potentials, and Kd values, detailed overview

685182

-

additional information

additional information

Acinetobacter baumannii

-

reaction kinetics of C2-FMNH with oxygen at various pH values investigated by stopped-flow and rapid quenched-flow techniques, detailed overview

712503

-

additional information

additional information

Acinetobacter baumannii

Q6Q272

reaction kinetics of C2-FMNH with oxygen at various pH values investigated by stopped-flow and rapid quenched-flow techniques, detailed overview

712503

-