Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

1.14.14.9: 4-hydroxyphenylacetate 3-monooxygenase

This is an abbreviated version!
For detailed information about 4-hydroxyphenylacetate 3-monooxygenase, go to the full flat file.

Word Map on EC 1.14.14.9

Reaction

4-hydroxyphenylacetate
+
FADH2
+
O2
=
3,4-Dihydroxyphenylacetate
+
FAD
+
H2O

Synonyms

4 HPA 3-hydroxyylase, 4-HPA hydroxylase, 4-hydroxyphenylacetate 3-hydroxylase, 4-hydroxyphenylacetic acid 3-hydroxylase, 4HPA 3-monooxygenase, 4HPA3H, C2-hpah, EC 1.14.13.3, HPA 3-hydroxylase, HpaB, hpaBC, HpaC, HPAH, More, p-hydroxyphenylacetate 3-hydroxylase, p-hydroxyphenylacetate hydroxylase, p-hydroxyphenylacetic 3-hydroxylase, TPY_2462, two-component p-hydroxyphenylacetate hydroxylase

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.14 With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.14.9 4-hydroxyphenylacetate 3-monooxygenase

Cloned

Cloned on EC 1.14.14.9 - 4-hydroxyphenylacetate 3-monooxygenase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21 Star (DE3) cells
-
expressed in Escherichia coli BL21(DE3) cells
-
expressed in Escherichia coli DH1
-
expressed in Escherichia coli DH1 and TG1
-
expression in Escherichia coli
expression in Escherichia coli K-12
-
expression in Saccharomyces cerevisiae
gene hpaB (oxygenase component), gene hbaC (reductase component), expressed in Escherichia coli
-
gene hpaH, DNA and amino acid sequence determination and analysis, sequence comparisons, expression in enzyme-deficient Escherichia coli strain DH5alpha
genes c1-hpah and c2-hpah, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21(DE3), the recombinant enzyme is similarly active cmpared to the native one
genes c1-hpah and c2-hpah, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21(DE3), the recombinant enzyme is similarly active compared to the native one
HpaA and HpaH are expressed in Escherichia coli W-21, CC118 and YS1 as well as Klebsiella pneumonia mutant strain AG813
-
hpaB gene is expressed in Escherichia coli BL21(DE3)
-