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1.14.14.40: phenylalanine N-monooxygenase

This is an abbreviated version!
For detailed information about phenylalanine N-monooxygenase, go to the full flat file.

Word Map on EC 1.14.14.40

Reaction

L-phenylalanine
+ 2 [reduced NADPH-hemoprotein reductase] + 2 O2 =
(E)-phenylacetaldoxime
 + 2 [oxidized NADPH-hemoprotein reductase] +
CO2
 + 3 H2O

Synonyms

CYP79A1, CYP79A2, CYP79D16, cytochrome P450 79D16, EC 1.14.13.124

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.14 With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.14.40 phenylalanine N-monooxygenase

Application

Application on EC 1.14.14.40 - phenylalanine N-monooxygenase

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APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
synthesis
biosynthetic pathway for the production of phenylacetonitrile in Escherichia coli utilizing enzymes from the plant glucosinolate-biosynthetic and bacterial aldoxime-nitrile pathways, i.e. introducing the genes encoding cytochrome P450 (CYP) 79A2 and CYP reductase from Arabidopsis thaliana, yielding the E,Z-phenylacetaldoxime-producing transformant and further introducing the aldoxime dehydratase (Oxd) gene from Bacillus sp. strain OxB-1, yielding the phenylacetonitrile-producing transformant. Expression of active CYP79A2 and concentration of biomass are improved by the combination of the autoinduction method, coexpression of groE, encoding the heat shock protein GroEL/GroES, N-terminal truncation of CYP79A2, and optimization of the culture conditions, yielding a more than 60fold concentration of E,Z-phenylacetaldoxime up to 2.9 mM